Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum

Authors(2) :-Rohini C. Yevale, Sushma G. Sabharwal

Peroxidases are the heme containing enzymes that catalyse oxidation of substrates like phenolic compounds by reducing hydrogen peroxide concurrently. They play a key role in a number of metabolic processes such as regulation of cell elongation, lignification of cell wall, phenolic oxidation and defence against stress. Peroxidase was isolated from 4 days old seedlings of Macrotyloma uniflorum followed by germination study. The enzyme showed optimum pH 6 and optimum temperature 30°C. Peroxidase showed a stability over a broad pH range (4 to 9). Enzyme also was found to be stable up to 70°C. Kinetic studies were also performed.

Authors and Affiliations

Rohini C. Yevale
Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India
Sushma G. Sabharwal
Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India

Peroxidase, Macrotyloma Uniflorum, Seedlings, Hydrogen Peroxide.

  1. Hayrunnisa Nadaroglu1*, Neslihan Celebi1, Nazan Demir2 and Yasar Demir 6 June, 2013 Purification and characterisation of a plant peroxidase from rocket (Eruca vesicaria sbsp. Sativa) (Mill.) (syn. E. sativa) and effects of some chemicals on peroxidase activity in vitro. African Journal of AgriculturalResearch Vol. 8(21), pp. 2520-2528.
  2. S.K.Vernwai, R.S.S.Yadav and K.D.S. Yadav , Augest 2006 Purification of a peroxidase freom Solanum melongena fruit juice African Journal of Agricultural vol. 43 pp. 239-243.
  3. Hermelinda Penha *Marcos Toyama, Marcus Smolka Gláucia Maria Pastore 2007 EXTRACTION, PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF A PEROXIDASE FROM Copaifera langsdorffii LEAVES Quim. Nova, Vol. 30, No. 5, 1067-1071.
  4. Reetesh Kumara, Kunwar Awaneesh Singhb, Vijay Kumar Singha, Medicherla V. Jagannadhama 2011, Biochemical characterization of a peroxidase isolated from Caribbean plant: Euphorbia cotinifolia Process Biochemistry 46 (2011) 1350–1357.
  5. Angelo vianello, Marco Uncani, Gabriellanagy, and Francesco Macrl:* (1997)Guaiacol Peroxidase Associated to Soybean Root Plasma Membranes Oxidizes Ascorbate J Plant PhysioL Ql. 150. pp. 573-577.
  6. Silvana De Leonardis, Nunzio Dipierro, Silvio Dipierro* Purification and characterization of an ascorbate peroxidas from potato tuber mitochondria
  7. Galila Hassan Onsa, Nazamid bin Saari*, Jinap Selamat, Jamilah Bakar 2004 Purification and characterization of membrane-bound peroxidases from Metroxylon sagu Food Chemistry 85 (2004) 365–37
  8. S.S. Deepa, C. Arumughan* 2002 Purification and characterization of soluble peroxidase from oil palm (Elaeis guineensis Jacq.) leaf Phytochemistry 61 (2002) 503–511.
  9. Takeshi Uchida,*, Miho Sasaki, Yoshikazu Tanaka, and Koichiro Ishimori October 2, 2015 A Dye-Decolorizing Peroxidase from Vibrio cholera Biochemistry 2015, 54, 6610−6621.
  10. Jianwen Wang , Ming Gu, Junwei Di, Yansheng Gao Ying Wu, Yifeng Tu 2007 A carbon nanotube/silica sol–gel architecture for immobilization of horseradish peroxidase for electrochemical biosensor Bioprocess Biosyst Eng, (2007) 30:289–296.
  11. Bum Joon Kim a, Bong Keun Kang a,b, Young Yil Bahk a, Kyung Hwa Yoo a,b, Kook Jin Lim (2009) Immobilization of horseradish peroxidase on multi-walled carbon nanotubes and its enzymatic stability,Current Applied Physics 9,263–265.
  12. Feng Zhang,Bin Zheng, Jiali Zhang, Xuelei Huang, Hui Liu, Shouwu Guo,and Jingyan Zhang, 2010, Horseradish Peroxidase Immobilized on Graphene Oxide: Physical Properties and Applications in Phenolic Compound Removal, J. Phys. Chem. C, 114, 8469–8473.
  13. Liande Zhu∗, Ruilan Yang, Jiangli Zhai, Chunyuan Tian (2007), Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode, Biosensors and Bioelectronics 23 528–535.
  14. Seyda Korkut, Bulent Keskinler, Elif Erhan Talanta, (2008), An amperometric biosensor based on multiwalled carbon nanotube-poly(pyrrole)-horseradish peroxidase nanobiocomposite film for determination of phenol derivatives,76 1147–1152
  15. Jin-Zhong Xu, Jun-Jie Zhu, Qiang Wu, Zheng Hu, Hong-Yuan Chen, An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode. (Futture article)
  16. P.George. 1953, Intermediate compound formation with peroxidase and strong oxidising agents, J.biochem, , 201, 413 -426.
  17. Agostini E, Medina MJ, Silvia R, Forchetti MD, Tigier H. 1997, Properties of anionic peroxidase isoenzymes from turnip (Brassica napus L.) roots. J Agric Food Chem, 45(3), 596–598.
  18. Khalid M. Naji1 and V. R. Devaraj (2009) FROM DEHYDRATED SEEDLINGS OF HORSE GRAM (Macrotyloma Uniflorum) TISSUE CULTURE, Faculty of Science Bulletin, 22 39-48.
  19. Pankaj Kumar Chaurasia, Sunil Kumar Singh, Shashi Lata Bharati , 2013, Study of peroxidase obtained from Daucus carota (carrot) juice extract, Journal of Applicable Chemistry, 2 (5):1123-1131
  20. Nigel C. Veitch, 2004, Structural determinants of plant peroxidase function, Phytochemistry Reviews 3: 3–18, Kluwer Academic Publishers. Printed in the Netherlands.

Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 208-213
Manuscript Number : IJSRST185141
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Rohini C. Yevale, Sushma G. Sabharwal, " Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.208-213, January-February-2018.
Journal URL : https://ijsrst.com/IJSRST185141
Citation Detection and Elimination     |      | | BibTeX | RIS | CSV

Article Preview