Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum

Authors(2) :-Rohini C. Yevale, Sushma G. Sabharwal

Peroxidases are the heme containing enzymes that catalyse oxidation of substrates like phenolic compounds by reducing hydrogen peroxide concurrently. They play a key role in a number of metabolic processes such as regulation of cell elongation, lignification of cell wall, phenolic oxidation and defence against stress. Peroxidase was isolated from 4 days old seedlings of Macrotyloma uniflorum followed by germination study. The enzyme showed optimum pH 6 and optimum temperature 30°C. Peroxidase showed a stability over a broad pH range (4 to 9). Enzyme also was found to be stable up to 70°C. Kinetic studies were also performed.

Authors and Affiliations

Rohini C. Yevale
Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India
Sushma G. Sabharwal
Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India

Peroxidase, Macrotyloma Uniflorum, Seedlings, Hydrogen Peroxide.

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Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 208-213
Manuscript Number : IJSRST185141
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Rohini C. Yevale, Sushma G. Sabharwal, " Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.208-213, January-February-2018.
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