Immobilisation and Applications of a Thermostable Lipase from the Seeds of Macrotylomauniflorum

Authors(2) :-Shashikant B. Gatkal, Sushma G. Sabharwal

Lipases (triacylglycerol acylhydrolases EC 3.1.1.3) have proved to be one of the most important industrial enzymes as they are able to perform in both aqueous and water-restricted environments catalyzing stereospecific hydrolysis and transesterification reactions respectively. Lipases from plant sources have interesting features for application in different fields. In the present study, a lipase from seeds of Macrotylomauniflorum was isolated and purified using hydrophobic interaction chromatography. The enzyme was found to be thermoactive, having maximum hydrolytic activity at 60o C and stability over the range of 20 to 70o C. The optimum pH for the enzyme is 8.6 and is stable in alkaline range upto pH 10. The enzyme was inhibited by Cd2+, Hg2+, tween 20, tween 40 as well as pesticides malathion, methyl parathion and chlorpyrifos. Immobilization of enzyme on calcium alginate beads has been done and its application in carrying out transesterification reactions in non-polar solvents has been investigated.

Authors and Affiliations

Shashikant B. Gatkal
Biochemistry Division, Department of Chemistry, SavitribaiPhule Pune University, Pune, Maharashtra, India
Sushma G. Sabharwal
Biochemistry Division, Department of Chemistry, SavitribaiPhule Pune University, Pune, Maharashtra, India

Lipase, Macrotylomauniflorum, Thermostable, Immobilization.

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Publication Details

Published in : Volume 5 | Issue 1 | January-February 2018
Date of Publication : 2018-01-20
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 225-230
Manuscript Number : IJSRST185144
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Shashikant B. Gatkal, Sushma G. Sabharwal, " Immobilisation and Applications of a Thermostable Lipase from the Seeds of Macrotylomauniflorum , International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 5, Issue 1, pp.225-230, January-February-2018. Available at doi : 10.32628/IJSRST185144
Journal URL : http://ijsrst.com/IJSRST185144

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