Evaluation of in vitro antioxidant potential of Carissa carandas genotype found in NE India

Authors

  • Dipanjali Saikia  Ph.D Scholar, Department of Biotechnology, Gauhati University, Guwahati, Assam, India
  • Dr. P.J. Handique  Professor, Department of Biotechnology, Gauhati University, Guwahati, Assam, India

DOI:

https://doi.org//10.32628/IJSRST19621

Keywords:

Carissa Carandas, Antioxidant, Radical Scavenging, DPPH, Reducing Power.

Abstract

The present work was designed to study the antioxidant potential of various parts of Carrisa carandas using different assay systems. C. carandas is a semi-wild shrubby plant species found in Assam (India). The species is being used in various traditional medicinal practices in Assam and other parts of NE India. The methanol extract of the fruits of C. carandas showed highest radical scavenging activity against DPPH (1, 1diphenyl-2-picryl hydrazyl). Ethanol extract of the fruits showed highest radical scavenging activity against H2O2. The study revealed that the fruits, leaves and seeds extracts of C. carandas possesses potent antioxidant activity. The antioxidant potential was determined to be in the following order: Fruit > Leaf > Seed extracts.

References

  1. B. Halliwell, M.A. Murcia, S. Chirico and O.I. Aruoma (1955). Free radicals and antioxidants in food and in vivo: what they do and how they work. Critical Reviews in Food Science and Nutrition; 35: 7-20.
  2. L.J. Machlin and A. Bendich (1987). Free radical tissue damages: protective role of antioxidant nutrients. FASEBJ; 1:441-445.
  3. M. Dusinska, J. Lietava, B. Olmedilla, K. Raslova, S. Southon, A.R. Collins, T.K. Basu, N.J. Temple and M.L. Garg (1999). Indicators of Antioxidative stress in Antioxidants and Human Health; New York, USA: CAB International; 411- 422.
  4. Dhan Prakash, Garima Upadhyay and P. Pushpangadan (2011). Antioxidant Potential of Some Under-Utilized Fruits.Indo-Global Journal of Pharmaceutical Sciences; 1: 25-32.
  5. P.J. Handique (2009). Medicinal Plants of North East India:Status Diversity Conservation Cultivation & Trade.Year: 2009, International Book Distributors, Dehradun; ISBN: 9788170893530 / 8170893534.
  6. Berhane Kidane, L.J.G. van der Maesen, Tinde van Andel, Zemede Asfaw and M.S.M. Sosef (2014). Ethnobotany of Wild and Semi-Wild Edible Fruit Species used by Maale and Ari Ethnic Communities in Southern Ethiopia. Ethnobotany Research & Applications 12:455-471.
  7. K.R. Kirtikar and B.D. Basu (2005). Indian Medicinal Plant, Vol I, 2nd Ed, Dehradun. International Book Distributors; 1546 -1548.
  8. D.M.A. Jayaweera (1981). Medical Plants used in Ceylon, Colombo. National Science Council of Srilanka; 42-49.
  9. V.H Bhaskar and N. Balakrishnan (2009). Analgesic, anti-inflammatory and antipyretic activities of Pergularia deamia and Carissa Carandas. DARU Journal of Pharmaceutical Sciences; 17(3):168-174.
  10. V.H. Bhaskar and N. Balakrishnan (2009). Karaunda (Carissa carandasLinn.)- As a phytomedicine: A review. Kongposh publications; 95–100.
  11. B.N. Dhawan and G.K. Patnaik (1985). Investigation on some new cardio-active glycosides. Indian drugs; 22 (6):285-290.
  12. R.S.L. Taylor, J.B. Hudson, N.P. Manandhar and G.H.N. Tower (1996). Antiviral Activities of Medicinal Plant of Southern Nepal. Journal of Ethno pharmacology; 53(2):97-104.
  13. Rajasekaran, V. Jeyasudh, B. Kalpana and B. Jayakar (1999). Preliminary Phytochemical and antipyretic evaluation of Carissa carandas L.; Indian J. of natural Prod., 15 (1): 27-29.
  14. M. Blois (1958). Antioxidant determinations by the use of a stable free radical. Nature.181:1199–1200. doi: 10.1038/1811199a0.
  15. R.J. Ruch, S.J. Cheng and J.E. Klaunig (1989). Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis; 10: 1003-1008.
  16. W. Yu, Y. Zhao and B. Shu (2004). The radical scavenging activities of radix puerariae isoflavonoids: A chemiluminescence study. Food Chem. 86:525–529. doi: 10.1016/j.foodchem.2003.08.037.
  17. A. Yildrin, M. Okay and V. Bilaloglu (2001). The antioxidant activity of leaves of Cydonia vulgaris. Turkish Journal of Medical Science; 31:23-27.
  18. S. Meir, J. Kanner, B. Akiri and S.P. Hadas (1995). Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agric. Food Chem.; 43: 1831-181.
  19. Aman Deep Kaur, Parminder Nain and Jaspreet Nain (2012). In vitro Antimicrobial and Antioxidant Activity of Gingko biloba Bark Extract. International Research Journal of Pharmacy. 3(6):2230-8407.
  20. D. Prakash, B.N. Singh and G. Upadhyay (2007). Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa); Food Chemistry 102: 1389-1393.
  21. Farah Aslam, Nasir Rasool, Md. Riaz, Md. Zubair, Komal Rizwan, Mazhar Abbas, Tanveer Hussain Bukhari and Iftikhar Hussain Bukhari (2011). Antioxidant, haemolytic activities and GC-MS profiling of Carissa carandas roots. International Journal of Phytomedicine; 3:567-578.
  22. J. Kubola, S. Siriamornpun and N. Meeso (2011).Phytochemicals, vitamin C and sugar content of Thai wild fruits; Food Chem. 126:972-98.
  23. S.I. Mamun, N. Shaheen, A. BasakTukun and Md. Mohiduzzaman (2012). Hydrophilic Antioxidant Capacities and Total Phenol Content of Seasonal Fruits of Bangladesh. Mal J Nutr.; 18(3):355-362.

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Published

2019-04-30

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Section

Research Articles

How to Cite

[1]
Dipanjali Saikia, Dr. P.J. Handique, " Evaluation of in vitro antioxidant potential of Carissa carandas genotype found in NE India, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 6, Issue 2, pp.13-25, March-April-2019. Available at doi : https://doi.org/10.32628/IJSRST19621