Bioactive Compound (Phenolic, Anthocianin, and Antioxidant) in Black Rice (Oryza sativa var. Pare Ambo) South Sulawesi

Authors(4) :-N Kiay, A B Tawali, M M Tahir, M Bilang

Cereals are widely found in Indonesia and used as foodstuffs. Nutrient content of cereals vary from quantity and quality. Rice, one kind of cereals is commonly consumed by Indonesian community. There are some various types of rice such as brown rice, black rice and red rice. One type of rice, namely black rice is widely known as antioxidant source, for example black rice. This research be focused on the analysis of bioactive compound in black rice (Oryza sativa var Pare Ambo). This research using laboratory experiment methods to analyze total phenolic content, anthocianin, and antioksidan (free radical scavenging activity DPPH) black rice using spectrophotometer UV-vis. This research using complete random design (rancangan acak lengkap, RAL), thus obtained data were analyzed using statistic. Significant difference between groups were analyzed using ANOVA with p<0,05. The results showed that black rice extract possess to total phenolic content 3,84 mg/kg gallic acid, free radical scavenging activity 81,20%, total antioxidant 28,42 ppm and vitamins B1, B5, B6 mg/100 g sample (p>0,05). Black rice possess to total phenolic content 10,26 mg/kg gallic acid, free radical scavenging activity 62,67%, anthocianin content 122,33%, red rice possess to total phenolic content (TPC) 7,48 mg/kg gallic acid, free radical scavenging activity 54,78%,. Antioxidant activity from black rice caused by it phenolics compound that act as antioxidant. Spectra XRD Analysis for Determination of Vitamin B (Percentage of Vitamins) (B3, B5, B6) niacin, pantothenic acid and Riboflavin results showed the highest percentage of vitamin B5 (40,45). The results of the research were 88,21% carbohydrates, 8.36% protein, 1.74% fat, 11.17% water, 1.15% ash which amounts to 0.395 mg calcium (Ca), 1.98 mg / ml Magnesium (Mg) and 0.387 mg / ml iron (Fe). In conclusion from this study, black rice has antioxidant activity and nutrient content, these products are expected to be native functional foods.

Authors and Affiliations

N Kiay
Post-Graduate Student, Hasanuddin University, Makassar, South Sulawesi, Indonesia
A B Tawali
Faculty of Agricultural, Gorontalo University, Gorontalo, Indonesia
M M Tahir
Department of Food Science and Technology, Hasanuddin University, Jl. PerintisKemerdekaan Km.10, Makassar, Indonesia
M Bilang
Department of Food Science and Technology, Hasanuddin University, Jl. PerintisKemerdekaan Km.10, Makassar, Indonesia

Phenolic Content, Anthosianin, free radical scavenging activity, Vitamins, Black Rice, XRD

  1. AOAC (Association of Official Analytical Chemyst) (2005). Official Method of Analysis of The Association of Official Analytical of Chemist. Arlington (USA): The Association of Official Analytical Chemist, Inc.
  2. Bouis HE, Chassy BM, Ochanda JO. 2003. Genetically modified food crops and their contribution to human nutrition and food quality. Trend Food Sci Tech 14:191–209
  3. Fitzgerald MA, McCouch SR, Hall RD. 2009. Not just a grain of rice: the quest for quality. Trend Plant Sci 14:133–139
  4. Gani, A., Wani SM, Masoodi FA and Gousia Hameed. 2012. Whole-Grain Cereal Bioactive Compounds and Their Health Benefits: (A Review) J Food Process Technol 2012, 3:3
  5. Hu C, Zawistowski J, Ling W, Kitts DD. 2003. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem 51: 5271-5277.
  6. Jun, H. I., Shin, J. W., Song, G. S., & Kim, Y. S. (2015). Isolation and identi?cation of phenolic antioxidants in black rice bran. Journal of Food Science, 80(2), C262–C268.
  7. Kim, G. R., Jung, E. S., Lee, S., Lim, S., Ha, S., & Lee, C. H. (2014) Combined mass spectrometry-based metabolite pro?ling of different pigmented rice (Oryza sativa L.) seeds and correlation with antioxidant activities. Molecules, 19,15673-5686.
  8. Krawinkel MB. 2007. What we know and don’t know about Golden Rice. Nat Biotechnol 25:623.
  9. Lees, D. H., & Francis, F. J. (1972). Standardization of pigment analyses in cranberries. HortScience, 7, 83–84.
  10. Lee, J. H. (2010). Identi?cations and quanti?cation of anthocyanins from the grains of black rice (Oryza sativa L.) varieties. Food Science and Biotechnology, 19,391–397.
  11. Martínez-Tome M, Murcia MA, Frega N, Ruggieri S, Jiménez AM. 2004. Evaluation of antioxidant capacity of cereal brans. J Agric Food Chem 52: 4690-4699.
  12. Park, Young Sam, Sun-Joong Kim, and Hyo- Ihl Chang. 2008. Isolation of Anthocyanin from Black Rice (Heugjinjubyeo) and Screening of its Antioxidant Activities Kor. J. Microbiol. Biotechnol. Vol. 36, No. 1, 55–60(2008)
  13. Perez-Jiménez J, Saura-Calixto F. 2005. Literature data may underestimate the actual antioxidant capacity of cereals. J Agric Food Chem 53: 5036-5040.
  14. Seal CJ (2006) Whole grains and CVD risk. Proc Nutr Soc 65:24–34Takaiwa F, Yang L, Yasuda H (2008) Health- promoting transgenic rice: application of rice seeds as a direct delivery system for bioactive peptides in human health. In: Hirano HY, Sano Y, Hirai A, Sasaki T (eds) Rice biology in the genomics era. Springer, Berlin pp 357–373
  15. Teramoto, Y.1*, Koguchi, M.1, Wongwicharn, A.2 and Saigusa, N. 2011. Production and antioxidative activity of alcoholic beverages made from Thai ou yeast and black rice (Oryza sativa var. Indica cv. Shiun) African Journal of Biotechnology Vol. 10(52), pp. 10706-10711,
  16. Vitaglione P, Napolitano A, Fogliano V (2008) Cereal dietary fibre: a natural functional ingredient to deliver phenolic compounds into the gut. Trend Food Sci Tech 19:451–463
  17. Xu Z, Hua N, Godber JS. 2001. Antioxidant activity of tocopherols, tocotrienols, and gamma- oryzanol components from rice bran against cholesterol oxidation accelerated by 2, 20-azo bis (2-methylpropionamidine) dihydrochloride. J Agric Food Chem 49: 2077-2081.
  18. Yu L, Haley S, Perret J, Harris M, Wilson J. 2002. Free radical scavenging properties of wheat extracts. J Agric Food Chem 50: 1619-1624.
  19. Yu L, Zhou k, Parry Jw. 2005. Inhibitory effects of wheat bran extracts on human LDL oxidation and free radicals. LWT 38: 463-470.
  20. Zieli Å, Ki H, Koz Å‚ Owska H. 2000. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J Agric Food Chem 48: 2008-2016.
  21. Winarno, F. G., F. Srikandidan D. Fardiaz. 1988. Food Technology Introduction. PT GramediaPustakaUtama. Jakarta
  22. Winarno, F. G., F. Srikandidan D. Fardiaz.. 2002Food and Nutrient Chemistry. Jakarta. PT GramediaPustakaUtama
  23. Zhang, H., Shao, S., Bao, J., & Beta, T. (2015). Phenolic compounds and antioxidant properties of breeding lines between the white and black rice. Food Chemistry, 172, 60–639.

Publication Details

Published in : Volume 6 | Issue 2 | March-April 2019
Date of Publication : 2019-04-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 143-150
Manuscript Number : IJSRST196222
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

N Kiay, A B Tawali, M M Tahir, M Bilang, " Bioactive Compound (Phenolic, Anthocianin, and Antioxidant) in Black Rice (Oryza sativa var. Pare Ambo) South Sulawesi", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 6, Issue 2, pp.143-150, March-April-2019. Available at doi : https://doi.org/10.32628/IJSRST196222    
Journal URL : https://ijsrst.com/IJSRST196222
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