Carotenoid Content and Weight Gain of Caulerpa racemosa (Chlorophyta, Caulerpaceae) at Several Light Intensities

Authors

  • Rajuddin Syamsuddin  Center of Excellence for Development and Utilization of Seaweed, Hasanuddin University (PUI-P2RL UNHAS), Indonesia
  • Rustam  Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Indonesia
  • Abustang  Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Indonesia
  • Idil Fitra  Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Indonesia

DOI:

https://doi.org//10.32628/IJSRST196252

Keywords:

Carotenoid, Caulerparacemosa, Low Light Intensity, Photoinhibition, Photorespiration, Weight Gain

Abstract

An experiment was conducted done outdoors in the area of the Wet Laboratory of Faculty of Marine Science and Fisheries, HasanuddinUniversity to examine the influence of several light intensity ranges on carotenoid content and weight gain of the seaweed Caulerparacemosa. Filtered seawater and styrofoam boxes were the cultivation medium and container, used respectvely. Carotenoid content and the weight gain was analyzed in the Water Productivity and Quality Laboratory of FIKP, UNHAS. Carotenoid content was determined spectrometrically, the weight gain, carotenoids content and water quality parameters were statistically and descriptively analyzed, respectively. Low light intensity of 200 – 300 lux was lower than the saturation point resultedlow weight gain due to photoinhibitionand decreased chlorophyll synthesis, greater respiration activity than photosynthesis.Light intensity range of 500 – 600 lux was exceed the saturation point causecarotenoid damage, low weight gain due to photorespiration, decreased nitrate reductase activity, cell damage and death, reducephotosyntheisi and reduce cell size. Light intensity range of 400 – 500 lux was the optimum and saturation point light intensityresultedhigh weight gain. The range of temperature, salinity, pH, ammonium concentration, and magnesium water media during the study was suitable the growth of C.racemosa.

References

  1. Andarias, I. 1992. Pengaruh takaran urea dan TSP terhadap produksi bobot kering klekap. Bulletin ilmu perikanan dan peternakan. Universitas Hasanuddin Makassar.
  2. Anderson J.M. 1986. Photoregulation of the composition, function, and structure of thylakoid membranes. Annual Review of Plant Physiology 37: 93-136
  3. Afrianto, E and E. Liviawaty. 1993. Budidaya Laut dan Cara Pengolahannya. Bharata. Jakarta.
  4. Azizah, R.T.N., 2006. Percobaan Berbagai Macam Metode Budidaya Latoh (C.racemosa) sebagai Upaya Menunjang Komunitas Produksi. Jurusan Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro, Kampus Temalang Semarang, Indonesia.
  5. Carruthers T.J.B., D.I Walker and J.M. Huisman . 1993. Culture studies on two morphological types of Caulerpa (Chlorophyta) from Perth, Western Australia, with a description of a new species. Botanica Marina 36: 589-596.
  6. Dawes, C. J. 1981. Marine Botany. John Wiley. And Sons University of South Florida. New York. 268 pp.
  7. Donatas Z., D., R. G. Hiller, V. Sundström, and T. Polívka. 2002. Carotenoid to chlorophyll energy transfer in the peridinin–chlorophyll-a–protein complex involves an intramolecular charge transfer state. Proceeding of the National Academy of Science of USA.; https://doi.org/10.1073/pnas.262537599
  8. Dring, M.J. 1971. Light Quality and Photimorphogenesis of Algae in Marine Environment. 14th European Marine Biology Symposium. Cambridge University Press, pp. 375-392.
  9. Fleurence, J. 1999. Seaweed Proteins; biochemical, nutritional aspects and potential uses.Trend in Food Science and Technology, 10; 25 – 28.
  10. Gammanpila, A.M., C.P. Rupasinghe, S. A Subasinghe 2015. Light Intensity and Photoperiod Effect on Growth and Lipid Accumulation of Microalgae Chlorella vulgaris and Nannochloropsis Sp for Biodiesel Production. Proceedingsof12thISERDInternationalConference,Tokyo,Japan,26thSept.2015,ISBN:978-93-85832-00-0
  11. Gardner, F.P. 1995. Fisiologi Tanaman Budidaya. Penerjemah: Susilo, H. Jakarta: UI Press.
  12. Geider, W. 2006. Fotosintesis Pada Alga dan Bakteri. Wikipedia.Indonesia. www.Google.com (24 January 2011. 18.40)
  13. Goericke, R. and J.P. Montoya. 1998. Estimating the contribution of microalgal taxa to hlorophyll a in the field – variations of pigment ratios under nutrient-and-light-limited growth. Marine EcologyProgress Series, 169, 97–112.
  14. Grant, C. 2011. Light Intensity Influence on Algal Pigments, Protein and Carbohydrates; Implications for Pigment-Based Chemotaxonomy. A Dissertation. Faculty of The Charles E. Schmidt College ofScience
  15. Havaux, M., J.P. Bonfils, C. Lütz, K.K. Niyogi. 2000. Photodamage of the photosynthetic apparatus and its dependence on the leaf developmental stage in the npq1 Arabidopsis mutant deficient in the xanthophyll cycle enzyme violaxanthin de-epoxidase. Plant Physiol. 2000;124:273–284.
  16. Iwamoto, H., G. Yonekawa and T. Asai. 1955 Fat Synthesis in Unicellular Algae. Part I. Culture Conditions for Fat Accumulation in Chlorella Cells. [Bull. Agr. Chem. Soc. Vol. 19, No. 4, 1955] pp.241-246
  17. Kabinawa, I. N. K.. 2006. Spirulina Ganggang Penggempur Aneka Penyakit. AgroMedia Pustaka. Jakarta. Hal 10.
  18. Kumar, M., J. Kulshreshtha and G. P. Singh. 2011 Growth and biopigment accumulation of Cyanobacterium Spirulinaplatensis at different light intensities and temperature. Braz. J. Microbiol. 2011:42(3):1128-1135
  19. Loera-Quezada, MM, G. Angeles, E.J. Qlguín. 2011. Effect of irradiance on the cell density, size and lipid accumulation of Neochlorisoleoabundans. Rev LatinoamBiotechnolAmb Algal 2: 81-92
  20. Mairh, O.P., U. Soe-Htun, and M.Ohno. 1986. Culture of Eucheuma striatum (Rodophyta, Solieraceae) in sub tropical of Shikoku,japan. Botanica Marina 29: 185-191.
  21. Marinho-Soriano, E., P.C. Fonseca, M.A.A.Carnerio, and W.S.C. Moreira. 2006. Seasonal Variation in the Chemical Composition . of Two Tropical Seaweeds. Bioresource Tech., 97:2402 - 2406
  22. Peni, D.K. Solichatun, dan E. Anggarwulan. 2003. Pertumbuhan, KadarKlorofil Karotenoid, Saponin, AktivitasNitratReduktaseAnting-anting (GA3)yang(Acalyphindica L.) pada Konsentrasi Asam Giberelat Berbeda.Jurusan Biologi,FMIPA. Universitas Sebelas Maret Surakarta. 57126.
  23. Pisal, D.S and S. S. Lele. 2004. Carotenoid production from microalga,Dunaliellasalina. Indian Journal of Biotechnology, Vol 4, October 2005, pp 476-483
  24. Richmond, A. 2004. Handbook of microalgal culture: Biotechnology and applied phycology. Blackwell Science Ltd., Oxford, UK
  25. Rudiger W, and F. L?pez – Figueroa. 1992. Photoreceptors in algae. Photochemistry and Photobiology 55: 949-954
  26. Salisbury, F.K. and C.Ross. 1969. Plant Physiology. Wadsworth Publ.Co.Inc. Belmon, California. 764 p.
  27. Schulter, L., F.Mohlenberg, H. Havskum, and S. Larsen. 2000. The use of phytoplankton pigments for identifying and quantifying phytoplankton groups in coastal areas: testing the influence of light and nutrients on pigment/chlorophyll a ratios. Marine Ecology Progress Series, 192, 49–63.
  28. Siefermann-Harms, D. 1980, The role of carotenoids in chioroplasts of higher plants, —In Biogenesis and Function of Plant Lipids (P. Mazliak, P. Benveniste, C, Costes, and R. Douce, eds), pp. 331-340, Elsevier/North-Holland Biomedical Press, ISBN 0-86689-01-6
  29. Steel, R.G.D., danJ.H,Torrie, 1993. Prinsip dan Prosedur Statistika, Suatu Pendekatan Biometrik. Terjemahan Bambang Sumantri, GramediaPustakaUtama, Jakarta.
  30. Stigum, V.M. 2012. The effect of light and temperatureon lipid production in microalgae. Master thesis. Deparmentof Biology. Program for Marine Biology and Limnology Universitetet, Oslo. 58p.
  31. Syrett, P.J. 1962. Nitrogen assimilation. In: Lewin, R.A. (Ed.). 1962. Physiology and biochemistry of algae. Acad. Press. New York. Pp. 171-188
  32. Taiz, L., and E. Zeiger. 2002. Plant Physiology.3rd. Ed. Sunderland; Sinauer Assoc. Pp.17-34
  33. Thirumaran, G. and P. Anantharaman. 2009. Daily Growth Rate of FieldFarming Seaweed Seaweed Kappaphycus alvarezii (Doty) Doty ex P. Silva in Vellar Estuary. World Journal ofFish and Marine Sciences 1 (3); 144-153. Annamalai University
  34. Van Oorschoot, J.L.P. 1955. Conversion of Light Energy in Algal Culture. H Veen man &Zonen – Wageningen. 277 p.
  35. Walsh, K., G. J. Jones. and R. H. Dunstan. 1997. Growth and metabolism In: PhytochemistryVolume 44, Issue 5, March 1997, Pages 817-82
  36. Ying Yang, 2013. Effects of Temperature, Light Intensity and Quality, Carbon Dioxide, and Culture Medium Nutrients on Growth and Lipid Production of Ettliaoleo abundans. A Dissertation. Worcester Polythechnic Institute. 151p.
  37. Xu, Y., I. M.Ibrahim,P. J.Harvey. 2016. The Influence of Photoperiod and Light Intensity on the Growth and Photosynthesis of Dunaliellasalina (Chlorophyta) .CCAP 19/30. Plant Physiology and Biochemistry106(2016)305-315

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Published

2019-04-30

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Research Articles

How to Cite

[1]
Rajuddin Syamsuddin, Rustam, Abustang, Idil Fitra, " Carotenoid Content and Weight Gain of Caulerpa racemosa (Chlorophyta, Caulerpaceae) at Several Light Intensities , International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 6, Issue 2, pp.436-444, March-April-2019. Available at doi : https://doi.org/10.32628/IJSRST196252