Insilico Analysis of PHB from Halophiles for potential Bio-medical applications

Abstract

Polyhydroxybutyrate (PHB) is an important biopolymer accumulated by halophilic organisms. PHA is a family of polyesters is accumulated as granules in the cell of bacteria. Polyhydroxybutyrate (PHB) can be used as an alternative polymer to polylactide-glycolides for drug carrier production. It is a linear homopolymer biosynthesized by various strains of bacteria by condensation of D (-)-B-hydroxybutyric acid and used as an energy and carbon source. PHB can be obtained by extraction from bacteria or by chemical synthesis. To be suitable as drug carrier the PHB (polymer) has to be biocompatible, biodegradable in certain applications, and nontoxic. PHB seems to be biocompatible and biodegrades readily to carbon dioxide in bacteria; however, in humans, the reports are few and contradictory. The PHB was extracted from halophilic bacteria. The structural characterization of PHB was done by using NMR (nuclear magnetic resonance). To genenerate SMILES the structure were drawn in MarvinSketch. The PHB were screened based on the Lipinski’s rule of 5. The PHB molecule was subjected to the toxicity analysis and those that passed the toxicity test analyzed for docking studies.