Green Synthesis of Copper Nanoparticles Using Mitragyna Parvifolia Bark Extract and its Antimicrobial Study

Abstract

In this study, Mitragyna parvifolia plant bark an aqueous extract which provides cost-effective, eco-friendly process, less time consuming, an environmentally benign, easy and proficient way for the synthesis of copper nanoparticles. Mitragyna parvifolia plant bark was collected from virpur hills forest area. The Mitragyna parvifolia plant bark extract was prepared in de-ionised water and used for the green synthesis of copper nanoparticles. The color change of the solution dark brown from pale yellow colored, this confirms that there is a formation of copper nanoparticles. The green synthesised copper nanoparticles were characterized by UV-Visible spectroscopy, FT-IR, XRD, SEM, TEM and their antimicrobial activity was investigated. From UV-Visible spectrophotometer result was confirmed the reduction of copper sulphate to copper nanoparticles. FTIR analysis was confirmed the bending vibrations and stretching bonds present in the sample. Spherical shape was finding out by XRD and the size of the particle was analyzed with the help of Scanning Electron Microscopy. The antibacterial activity experiment against Escherichia coli gram-negative and Bacillus subtilis gram-positive bacteria by agar well method and the maximum zone of inhibition was higher in gram-positive bacteria compared to gram-negative bacteria. The green synthesised copper nanoparticles proved to be potential candidates for medical application antimicrobial activity is highly essential.