In-Vitro Cytotoxicity Assay of Curry Leaves Silver Nanoparticles Against Thp-1 Cell Line

Authors(2) :-Dipraj Gaikwad, Shubhangi Puranik

Medicinal plants are major crop in India and backbone of the Indian agriculture industry. It is very easily available in local market. We can produce very effective drug against various disorders like HIV, cancer, Skin disease, etc. To overcome these disorders, drugs can be prepared using combination of crude extract and nanotechnology. Nanoparticles can be synthesis by chemical, Physical, Biological method. The Biological method is most promising of all. In this study curry leaves was the biological material used for synthesis of NPs. Curry leaves and AgNO3 were the important component for the synthesis. The synthesized silver nanoparticles were characterized by UV-Vis spectrometry which showed the presence of nanoparticles. SEM which showed that nanoparticles were spherical in shape, X-RD which showed that size of nanoparticles was range of 1- 239.6nm, FTIR which showed the alkyl halides, Alkenes functional groups on nanoparticles. It has been seen that curry leaves plays an important role in the reduction and stabilization of silver to silver nanoparticles. Further, the anticancerous activity of these silver nanoparticles was studied on THP-1 cancerous cell lines using MTT assay. In cancer, some of the body cells begin to divide without stopping and spread into surrounding tissue. MTT dye stains mitochondrial succinate dehydrogenase enzyme in living cells. Using this process about 90% anticancerous activity was seen. This shows the wide application of curry leave silver nanoparticles in field of oncology.

Authors and Affiliations

Dipraj Gaikwad
Department of Animal Biotechnology Modern College of Agricultural Biotechnology, Kule-Dakhane, Mulshi, Pune, Maharashtra, India
Shubhangi Puranik
Department of Animal Biotechnology Modern College of Agricultural Biotechnology, Kule-Dakhane, Mulshi, Pune, Maharashtra, India

Curry leaves, AgNO3, X-RD, SEM, FTIR, UV-Vis spectroscopy, THP-1 cell line, MTT Assay

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Publication Details

Published in : Volume 7 | Issue 1 | January-February 2020
Date of Publication : 2020-01-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 45-52
Manuscript Number : IJSRST207110
Publisher : Technoscience Academy

Print ISSN : 2395-6011, Online ISSN : 2395-602X

Cite This Article :

Dipraj Gaikwad, Shubhangi Puranik, " In-Vitro Cytotoxicity Assay of Curry Leaves Silver Nanoparticles Against Thp-1 Cell Line", International Journal of Scientific Research in Science and Technology(IJSRST), Print ISSN : 2395-6011, Online ISSN : 2395-602X, Volume 7, Issue 1, pp.45-52, January-February-2020.
Journal URL : https://ijsrst.com/IJSRST207110
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