Determination of Minimum Inhibiotry Concentration and Antibiotic Suspecibilty of Zinc Resistant Bactreia from Industrial Waste Water and Soil

Authors

  • Tiwari SR.  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India
  • Goregaonkar SS.  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India
  • Doifode SH.  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India
  • Sarda PD.  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India
  • Prashar K.  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India
  • Warkhade BB  Department of Biotechnology, Badrinarayan Barwale Mahavidyalaya, Jalna, Maharashtra, India

Keywords:

Zinc Resistant, Minimum Inhibitory Concentration (MIC), Antibiogram.

Abstract

Environmental pollution with toxic heavy metals is spreading throughout the world along with industrial process and accumulated in soil and waste water. Microorganism have developed several mechanism to tolerate such high concentration of heavy metal for this purpose screening of bacteria having tolerance to heavy metal zinc had been attempted in the present study. Total six zinc resistant bacteria isolated from the industrial waste water and soil. Based on morphological and biochemical characterization isolates were identified as Staphylococcus aureus. (W1, S1) Bacillus Spp. (W2, S2), and E.coli (W3, S3). Minimum inhibitory concentration (MIC) of the isolates were studied and it was found that E.coli (W3) strain have high resistance against zinc (MIC-1200?g/ml). The antibiogram was done and it was found that metal resistant bacteria exhibited high resistant pattern towards a group of antibiotics.

References

  1. Ahemad, M., & Khan, M. S. (2011). Pesticide interactions with soil micro flora: importance in bioremediation. In Microbes and Microbial Technology (pp. 393-413). Springer New York.
  2. Ahemad, M., & Malik, A. (2011). Bioaccumulation of heavy metals by zinc resistant bacteria isolated from agricultural soils irrigated with wastewater. Bacteriol J, 2, 12-21.
  3. Bauer RW, Kirby MD, Sherris JC and Turck M (1966) Antibiotic susceptibility testing by standard single disc diffusion method. American Journal of Clinical Pathology, 45:493-496.
  4. El-Deeb, B. (2009). Plasmid mediated tolerance and removal of heavy metals by Enterobacter sp. Am J Biochem Biotechnology, 5(1), 47-53.
  5. Holt JG, Krieg RN, Sneath AHP, Staley TJ, Williams TS (1994) Bergey's Manual of Determinative Bacteriology, 9th Edition. (International Edition)
  6. Joshi, B. H. and Modi, K. G. (2013). Screening and characterization of heavy metal resistant bacteria for itsprospects in bioremediation of contaminated soil. Journal of Environmental Research and Development, 7(4A), 1531.
  7. Mustapha MU and Halimoon N (2015) Screening and isolation of heavy metal tolerant bacteria in industrial effluent. Procedia Environmental Sciences, 30, 33-37.
  8. Nath, S., Deb, B., & Sharma, I. (2012). Isolation and characterization of cadmium and lead resistant bacteria. Global Advanced Research Journal of Microbiology, 1(11), 194-198.
  9. Nikaido, H. (2009). Multidrug resistance in bacteria. Annual review of biochemistry, 78, 119-146.
  10. Pandey, B., & Fulekar, M. H. (2012). Bioremediation technology: a new horizon for environmental clean-up. Biology and Medicine, 4(1), 51.
  11. Sheik, C. S., Mitchell, T. W., Rizvi, F. Z., Rehman, Y., Faisal, M., Hasnain, S.& Krumholz, L. R. (2012). Exposure of soil microbial communities to chromium and arsenic alters their diversity and structure. PloS one, 7(6), e40059
  12. Singh, S., Kashyap, N., Singla, S., Bhadrecha, P., & Kaur, P. (2015). Bioremediation of heavy metals by employing resistant microbial isolates from agricultural soil irrigated with industrial waste water. Oriental Journal of Chemistry, 31(1), 357-361.
  13. Vidali, M. (2001). Bioremediation. An overview. Pure and Applied Chemistry, 73(7), 1163-1172.

International Journal of Scientific Research in Science and Technology

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Published

2018-01-30

Issue

Volume 4, Issue 1, January-February-2018

Section

Research Articles

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How to Cite

[1]
Tiwari SR., Goregaonkar SS., Doifode SH., Sarda PD., Prashar K., Warkhade BB, " Determination of Minimum Inhibiotry Concentration and Antibiotic Suspecibilty of Zinc Resistant Bactreia from Industrial Waste Water and Soil, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 1, pp.39-42, January-February-2018.