Antimicrobial, Antioxidant and Anti-Inflammatory Activities of the extract of a Moroccan endemic Narcissus: Narcissus broussonetii

Authors

  • Amal Razik  Laboratory of Biochemistry, Cellular and Molecular Biology, Faculty of Sciences, Casablanca, Morocco
  • Farida ADLY  Laboratory of Biochemistry, Cellular and Molecular Biology, Faculty of Sciences, Casablanca, Morocco
  • Mina MOUSSAID  Laboratory of Organic Synthesis and Biological Studies, Faculty of Sciences, Casablanca, Morocco
  • Chadi BERHAL  Laboratory phytosanitary Faculty of Agriculture, Beirut, Lebanon
  • Hassan MOUSSAID  Laboratory of Pharmaceutical Chemistry, Institute of Pharmacy, Brussels, Belgium
  • Abdelaziz ELAMRANI  Laboratory of synthesis, extraction, and Physico-chemical study of organic molecules, Faculty of Sciences Aînchock, Casablanca, Morocco
  • Touria Ould BELLAHCEN  Biology Department, Faculty of Sciences Ain-chock, Casablanca, Morocco
  • Nourdinne BOURHIM  Laboratory of Biochemistry, Cellular and Molecular Biology, Faculty of Sciences, Casablanca, Morocco
  • Jamal Jamal EDDINE  Laboratory of synthesis, extraction, and Physico-chemical study of organic molecules, Faculty of Sciences Aînchock, Casablanca, Morocco
  • Carles CODINA  Department of Natural Products, Plant Biology and Soil Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
  • Jaume BASTIDA  Department of Natural Products, Plant Biology and Soil Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
  • Mohamed LOTFI  Laboratory of Biochemistry, Cellular and Molecular Biology, Faculty of Sciences, Casablanca, Morocco

Keywords:

Amaryllidaceae, Narcissus broussonetii, alkaloids, pharmaceutical activities.

Abstract

The antioxidant, antibacterial and anti-inflammatory activities, of the extract of the Narcissus broussonetii bulbs were studied. The report concerning those properties of this Moroccan endemic species is described for the first time. Nine main alkaloids compounds were identified by GC and GC-MS such as tazettine, pretazettine, homolycorine, lycorine, ismine, 3-epimacronine and papiramine. The extracts of N. broussonetii up to 40 mg/100g b.wt, revealed a significant anti-inflammatory effect in mice and showed also a significant scavenging activity at 10 mg mL-1. However, the extractshowed a moderate antibacterial activity against all the microorganisms tested.

References

  1. Angiosperm Phylogeny Group. 2003. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Bot. J. Linn. Soc., 141: 399-436.
  2. Fernandes, A. 1951. Sur la phylogénie des espèces du genre: Narcissus L. Bol. Soc. Brot. 25:148
  3. Meerow A.W., Fay M. F., Chase M.C., Guy C.L and Li Q-B. 2000. The New Phylogeny of the Amaryllidaceae. Herbertia., 54: 180-203.
  4. Lloyd D.G., Webb C.J., Dulberger R. 1990. Heterostyly in species of Narcissus (Amaryllidaceae), Hugonia (Linaceae) and other disputed cases. Plant Syst. Evol., 172: 215-227.
  5. Meerow A.W., Fay M. F., Guy C.L., Li Q-B., Q Zaman F and Chase M.C. 1999. Systematics of Amaryllidaceae based on cladistic analysis of plastid sequence data. Am. J. Bot., 86: 13-25.
  6. Worley A.C., Baker A.M., Thompson J.D and Barrett S.C.H. 2000. Floral display in Narcissus: variation in flower size and number at the species, population, and individual levels. Int. J. Plant Sci., 161: 69-79.
  7. Meerow A. W and Snijman D.A. 2001. Phylogeny of Amaryllidaceae tribe Amaryllideae based on nrDNA ITS sequences and morphology. Am. J. Bot., 88: 2321-2330.
  8. Dominicis RI., D'Amato G and Tucci GF. 2002. On the hybrid origin of Narcissus biflorus (Amaryllidaceae): analysis of C-banding and rDNA structure. Caryologia., 55(2).
  9. Fennane M. et Ibn Tattou M. 1998. Catalogue des plantes vasculaires rares, menacées ou endémiques du Maroc. Bocconea 8:193.
  10. Jennewein S and Croteau R. 2001. Taxol: biosynthesis, molecular genetics, and biotechnological applications. Appl. Microbiol. Biotechnol., 57: 13-19.
  11. Ingkaninan K., Irth H., Verpoorte R. 2002. Screening of Amaryllidaceae for biological activities: Acetylcholineesterase inhibitors in Narcissus. The genus Narcissus. G.R. Hanks (Editor) Harwood Publishers, London., 369-379.
  12. De Andrade J. P, Pigni N. B, Torras-Claveria L, Berkov S, Codina C, Viladomat F , Bastida J. 2012. Bioactive alkaloid extracts from Narcissus broussonetii: Mass spectral studies Journal of Pharmaceutical and Biomedical Analysis., 70: 13– 25
  13. Jahandiz, E. and Maire R. 1934. Catalogue des plantes du Maroc. Tome III, imprimerie Minerva, Alger, Algérie (12 bis) J. Labra˜na, A.K. Machocho, V. Kricsfalusy, R. Brun, C. Codina, F. Viladomat, J. Bastida, Alkaloids from Narcissus angustifolius subsp. transcarpathicus (Amaryllidaceae), Phytochemistry 60 (2002) 847–852.
  14. Bastida J., Lavilla R and Viladomat F. 2006. Chimical and biological aspects of Narcissus alkaloids, The Alkaloids: J. Chem and Biol., 63: 87–179.
  15. Viladomat F. Bastida J, Tribó G, Codina C, Rubiralta M. 1990. Alkaloids from Narcissus bicolor, Phytochemistry., 29: 1307–1310.
  16. Berkov S, Bastida J, Sidjimova B, Viladomat F, Codina C. 2008. Phytochemical differentiation of Galanthus nivalis and Galanthus elwesii (Amaryllidaceae): a case study, Biochem. Syst. Ecol., 36 : 638–645.
  17. Peiwu L., Hopia A., Jari S., Yrjnen T and Vuorela H. 1999. TLC method for evaluation of free radical scavenging activity of Rapeseed meal by video scanning technology. Proceedings of the 10th International Rapeseed Congress, Canberra, Australia.
  18. Govindaragan R., Rastogi S., Vijayakumar M., Shirwaikar A., Rawat A.K.S., Mehrotra S and Pushpangadan P. 2003. Studies on the antioxidant activities of Desmodium gangeticum. Biol. Pharm. Bull., 26: 1424-1427.
  19. Winter C.A., Risley E.A and Nuss G.W. 1962. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drug. Proc. Soc. Exp. Biol. Med., 111: 544-547.
  20. Vinegar R., Truax J.F and Selph J.L. 1976. Quantitative comparison of the analgesic and anti-inflammatory activities of aspirin, phenacetin and acetaminophen in rodents. Eur. J. Pharmacol., 37: 23-30.
  21. Meng L., Mohan R., Kwok B.H.B., Elofsson M., Sin N and Crews C.M. 1999. Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo anti-inflammatory activity. Proc. Natl. Acad. Sci., 96: 10403-10408.
  22. Obukowics M.G., Welsch D.J., Salsgiver W.J., Martin-Berger C.L and Chinn K.S. 2005. Novel, selective 6 or 5 fatty acid desaturase inhibitors as anti-inflammatory agents in mice. J. Pharmacol. Exp. Ther., 287: 157-166
  23. Chabbert Y.A., Daguet G. L. 1985. Techniques en bactériologie : antibiotiques en bactériologie médicale. Editeur: Flammarion, Médecine & Sciences, Sérologie bactérienne.
  24. Vanden Berghe D.A., Vlietinck A.J., 1991. Screening methods for antibacterial and antiviral agents from higher plants. In: Hostettmann K. Ed., Methods in plant biochemistry, vol. 6. London, UK, Academic Press, p. 47-69.
  25. Armitage P. 1971. Statistical Methods in Medical. Black Well Scientific Publications, London.F. Damerau, A technique for computer detection and correction of spelling errors. Communications of the ACM, 1964, Vol. 7, Issue 3, pp. 659-664.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2016-02-25

Issue

Volume 2, Issue 1, January-February-2016

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Amal Razik, Farida ADLY, Mina MOUSSAID, Chadi BERHAL, Hassan MOUSSAID, Abdelaziz ELAMRANI, Touria Ould BELLAHCEN, Nourdinne BOURHIM, Jamal Jamal EDDINE, Carles CODINA, Jaume BASTIDA, Mohamed LOTFI "Antimicrobial, Antioxidant and Anti-Inflammatory Activities of the extract of a Moroccan endemic Narcissus: Narcissus broussonetii" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 2, Issue 1, pp.06-11, January-February-2016.