Detection of Anaerobic Bacteria in Bronchoalveolar Lavage of Patients with Chronic Chest Lesion

Authors

  • Ahmed M. Wahba  Department of Medical Microbiology, Beni Suef Faculty of Medicine Beni Suef University, Egypt
  • Mona A. Abdel-Messih  Department of Medical Microbiology, Cairo Faculty of Medicine Cairo University, Egypt
  • Randa S. Mohamed  Department of Chest, Beni Suef Faculty of Medicine Beni Suef University, Egypt
  • Naglaa A. Radi  Department of Medical Microbiology, Beni Suef Faculty of Medicine Beni Suef University, Egypt

DOI:

https://doi.org/10.32628/IJSRST196469

Keywords:

Anaerobe, Bronchoalveolar Lavage and Lung Abscess

Abstract

Anaerobic bacteria have important roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. The objectives of this study is to detect anaerobic bacteria in bronchoalveolar lavage samples of patients with chronic chest Lesion in chest department in Beni -Suef university hospital.

Patients & Methods : Each sample taken was placed immediately in previously prepared vacutainer containing thioglycolate media. Specimens from Thioglycollate broth after incubation were cultured on Brucella agar (Thermo Scientific™ Oxoid™) supplemented with 5% lysed sheep blood, hemin and Vitamin K1 . The plates were incubated at 37° c for 48-27 h in anaerobic condition. Commercial Kits RapID™ANA II System (Thermo Scientific™ Remel™ ) used for the identification of anaerobic isolates. Antimicrobial susceptibility testing for anaerobic isolates were done using MIC Test Strip (Liofilchem, Italy).

Results : The isolated anaerobic organism from BAL specimens were 7 out of 25(28%). The anaerobic isolates were Prevotella (3), Veillonella (2), Lactobacillus (1) and Propionibacterium (1).

Conclusion : Anaerobic bacteria are the most overlooked bacterial pathogens of the lower respiratory tract. They are often infrequently recovered from this site probably because of improper specimen collection and lack of appropriate anaerobic identification techniques.

References

  1. Bartlett J.G, (2012):Anaerobic bacterial infection of the Lung. Anaerobe. Volume 18, Issue 2, April2012, Pages235-239.
  2. Brook I. and Frazier.H. (1993): Aerobic and anaerobic microbiology of empyema. A retrospective review in two military hospitals Chest, 103 (1993), pp. 1502-1507.
  3. Mikamo H., Arakawa S., Fujiwara M., Funada H., et al. (2011): Anaerobic infections (individual fields): respiratory infections. Japanese Society of Chemotherapy Committee on guidelines for treatment of anaerobic infections; Japanese Association for Anaerobic Infection Research. J Infect Chemother. 2011 Jul;17 Suppl 1:42-6. doi: 10.1007/s10156-010-0137-6.
  4. Clinical and Laboratory Standards Institute (CLSI) (2017: Available online: https://clsi.org/standards/products/ microbiology/ (accessed on 10 September 2017).
  5. Tanaka S., Yoshida M., Murakami Y., et al. (2008): "The relationship of Prevotella intermedia, Prevotella nigrescens and Prevotella melaninogenica in the supragingival plaque of children, caries and oral malodor". J Clin Pediatr Dent. 32 (3): 195-200.
  6. Enfield K.B. and Sifri C.D. (2017): Aspiration, Empyema, Lung Abscesses, and Anaerobic Infections. Fishman's Pulmonary Diseases and Disorders, 5edition Chapter 127 .McGraw-Hill Medical.
  7. Bartlett J.G. (1993): Anaerobic bacterial infections of the lung and pleural space Clin Infect Dis, 16 (Suppl. 4) (1993), pp. S248-S255.
  8. Wang J.L., Chen K.Y. , Fang C.T., Hsueh P.R. , Yang P.C., Chang S.C. (2005):Changing bacteriology of adult community-acquired lung abscess in Taiwan: Klebsiella pneumoniae versus anaerobesClin Infect Dis, 40 (2005), pp. 915-922.
  9. Mori T., Ebe T., Takahashi M., Isonuma H, Ikemoto H. and Oguri T. (1993): Lung abscess: analysis of 66 cases from 1979 to 1991.Intern Med, 32 (1993), pp. 278-284.
  10. Takayanagi N., Kagiyama N., Ishiguro T., Tokunaga D. and SugitaY. (2010): Etiology and outcome of community-acquired lung abscess. Respiration, 80 (2010), pp. 98-105.
  11. De A., Varaiya A. and Mathur M. (2002):Anaerobes in pleuropulmonary infections Indian J Med Microbiol, 20 (2002), pp. 150-152.
  12. 12-Kuhajda I., Zarogoulidis K., Tsirgogianni K., Tsavlis D., et al., (2015): Lung abscess-etiology, diagnostic and treatment options. Ann Transl Med. 3(13): 183.doi: 10.3978/j.issn.2305-5839.2015.07.08.
  13. Blumer J.L., Saiman L., Konstan M.W. and Melnick D. (2007): The efficacy and safety of meropenem and tobramycin vsceftazidime and tobramycin in the treatment of acute pulmonary exacerbations in patients with chronic chest lesions.Chest 2007;128:233-2346.
  14. Snydman D.R., Jacobus N.V., McDermott L.A., Golan Y., et al. (2011): Update on resistance of Bacteroides fragilis group and related species with special attention to carbapenems 2006-2009.Anaerobe, 17 (2011), pp. 147-151.
  15. Sherrard L.J., Graham K.A., McGrath S.J., McIlreavey L., Hatch J., et al., (2013): Antibiotic resistance in Prevotella species isolated from patients with cystic fibrosis. J Antimicrob Chemother. 2013 Oct; 68(10): 2369-2374.
  16. Gupta A., Vlamakis H., Shoemaker N. and Salyers A.A. (2003): A new Bacteroides conjugative transposon that carries an ermB gene. Appl. Environ. Microbiol. 2003, 69, 6455-6463
  17. Khassebaf J., Hellmark B., Davidsson S., Unemo M., Nilsdotter-Augustinsson Å. and Söderquist B. (2015): Antibiotic susceptibility of Propionibacterium acnes isolated from orthopaedic implant-associated infections. Anaerobe. 2015 Apr;32:57-62.
  18. Hassan I.A., Hassan M.A., Embarek M.S., Attallah D.A., El Mokhtar M.A., Alaa Eldin G.M. (2015): Antibiotic Susceptibility Patterns of Propionibacterium Acnes Isolated from Acne Vulgaris in Assiut University Hospitals, Egypt Egyptian Journal of Medical Microbiology Volume 24 / No. 4 / October 2015 67-7.
  19. Lewis K. (2013): Platforms for antibiotic discovery. Nat. Rev. Drug Discov. 2013, 12, 371-387.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2019-08-31

Issue

Volume 6, Issue 4, July-August-2019

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Ahmed M. Wahba, Mona A. Abdel-Messih, Randa S. Mohamed, Naglaa A. Radi "Detection of Anaerobic Bacteria in Bronchoalveolar Lavage of Patients with Chronic Chest Lesion" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 6, Issue 4, pp.345-350, July-August-2019. Available at doi : https://doi.org/10.32628/IJSRST196469