Identification of Bacteria from under Finger Nails

Authors

  • Saloni Singh  Patna Women's College, Patna, Bihar, India
  • Dr. Jaya Philip  Patna Women's College, Patna, Bihar, India
  • Kumari Nidhi  Patna Women's College, Patna, Bihar, India
  • Pallavi Kumari  Patna Women's College, Patna, Bihar, India
  • Shagufta Akhtar  Patna Women's College, Patna, Bihar, India
  • Khushboo Kumari  Patna Women's College, Patna, Bihar, India

DOI:

https://doi.org/10.32628/IJSRST207629

Keywords:

Bacteria, Isolate, Microbes, Fingernails, Sterilized, Nutrient Agar, Incubated, Test, Inoculum, Colonies.

Abstract

This study was conducted to isolate and identify bacteria from human finger nails. A total of three nail samples were collected. The samples were collected from random people in which two of them were from females and one was from male. The isolated pathogens from finger nails include Bacillus species (2 isolates), coccus species (one isolate). Highest contamination of Bacillus species was isolated. After the colonies were being isolated, they were further characterized on the basis of biochemical characteristics including Indole test, Citrate test, Nitrate reduction test and Urease test. After that Antimicrobial susceptibility tests were performed to identify the resistance of a particular bacteria towards a given antibiotic i.e. Ampicillin, Chloromphenicol, Norfloxacin, Co-Trimoxazol and Ciprofloxacin. However the results obtained showed the resistance of isolates towards Ampicillin indicating the prevalance of potentially disease causing microbes under fingernails. This study showed the importance of nail hygiene.

References

  1. Aneja K., (2017). Experiments in microbiology, plant pathology, tissue culture and microbial biotechnology. 5th ed. New age international publishers. New Delhi. pp. 240-268.
  2. Aneja K., (2018). Laboratory manual of microbiology and biotechnology. 2nd ed. New age international publishers. New Delhi. pp. 242-270.
  3. Baran R., (2002). Nail healthy therapy: an attractive enhancement or a potential hazard. Journal of cosmetics and dermatology,1:24-29.
  4. Brannon M., Heather D., (2008). Nail anatomy. Journal of dermatology,122(7):192-199.
  5. Clarke, P.H., and S.T. Cowan. Biochemical methods for bacteriology. J. Gen. Microboil. 1952: 6:187-197
  6. Cohen., Philip R., Richard K., (1992). Nail disorders: diagnosis and treatment. Journal of the American academy of dermatology.26(4):521-531.
  7. Hedderwick S., Shelly M., Micheal L., Carol K., (2007). Pathogenic organisms associated with artificial fingernails worn by healthcare workers, journal of infection control and hospital epidemiology,21:505-509.
  8. Hunter J., Savin J., and Dahl M., (2002). Clinical dermatology. Malden, Mass; Blackwell sciences. pp. 173.
  9. Kumar A., Chordia N., (2017). Role of microbes in human health. Applied microbiology: open acess,3(2):2471-9315.
  10. Larson E., (2001). Hygiene of skin: when is clean too clean? Journal of emerging infectious diseases,7(2):12-17.
  11. Mengist A., Aschale Y., Reta A., (2018). Bacterial and parasitic assessment from fingernail in Debre markos, northwest Ethiopia. Canadian journal of infectious disease and medical microbiology.
  12. Michael j. Pelczar., ECS Chan, Noel R. Krieg.McGraw company New York Fifth editin. 1986: 597.
  13. Prescott L., Harley P., Kelvin D., (2005). Microbiology. 6th ed. Tim McGraw Hill co. New Delhi. pp: 675.
  14. Risan., Hasim., Mohsen., (2017). Isolation and identification of bacteria from under fingernails. International journal of current microbiology and applied science. pp:3584-3590.
  15. Wachukwu C., Abbey S., Obilor L., (2007). Public health implication of artificial finger nails used by health workers and food handlers. Journal of applied science,7(22):3580-3583. 
  16. Wang B., Johnson A., (2016). keratin: structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration.76: 229-318.
  17. Wootton M., Brown N., Glynn G., Teale C., (2019). BSAC methods for antimicrobial testing. BSAC British society for antimicrobial chemotherapy. pp.18-25.

International Journal of Scientific Research in Science and Technology

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Published

2020-12-30

Issue

Volume 7, Issue 6, November-December-2020

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Saloni Singh, Dr. Jaya Philip, Kumari Nidhi, Pallavi Kumari, Shagufta Akhtar, Khushboo Kumari "Identification of Bacteria from under Finger Nails" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 7, Issue 6, pp.234-241, November-December-2020. Available at doi : https://doi.org/10.32628/IJSRST207629