Diversity of Endophtic Bacteria in Aerides crispa, an Epiphytic Orchid

Authors

  • Darsha S  Mangalore University, Department of Research and Studies in Microbiology, PG Centre, Jnana Kaveri Campus, Kodagu, Karnataka, India
  • Jayashankar M  Mangalore University, Department of Research and Studies in Microbiology, PG Centre, Jnana Kaveri Campus, Kodagu, Karnataka, India.

DOI:

https://doi.org//10.32628/IJSRST21822

Keywords:

Aerides crispa, endophytic bacteria, 16S rDNA, similarity matrix, phylogenetic tree, bioconservation

Abstract

Aerides crispa is an epiphytic flowering orchid belongs to the family Orchidaceae.we have identified five different endophytic bacteria from this orchid. Genotypic characterization of the bacterial culture was done using 16S rDNA sequencing after PCR amplification. These sequences were compared with the known similar bacterial sequences from the NCBI GenBank database. Homological relationship of the bacteria was also compared by Phylogenetic tree generation. Bacteria obtained were Bacillus pumilus MT463728, Bacillus megaterium MT540506, Lysinibacillus fusiformis MT540507, Bacillus cereus MT540510, Aneurinibacillus migulanus. From the study, we have molecularly characterized and documented five different endophytic bacteria from the epiphytic orchid, A crispa. This shows the diversity of endophytes in orchids which speaks a lot about the urgent necessity of bioconservation without words.

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International Journal of Scientific Research in Science and Technology

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Published

2021-04-30

Issue

Volume 8, Issue 2, March-April-2021

Section

Research Articles

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How to Cite

[1]
Darsha S, Jayashankar M, " Diversity of Endophtic Bacteria in Aerides crispa, an Epiphytic Orchid, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 8, Issue 2, pp.07-16, March-April-2021. Available at doi : https://doi.org/10.32628/IJSRST21822