Effect of Semen Freezing and Thawing on Sperm Survival And Motility Rate : A Comparative Analysis

Authors

  • Ram Dayal  Department of Biotechnology, Singhania University, Pacheri Bari, Jhunjhunu, Rajasthan, India
  • Sumer Singh  School of Life Sciences, Singhania University,Pacheri Bari, Jhunjhunu, Rajasthan, India
  • Sunil K Thakur  Origin LIFE Healthcare Solutions and Research Centre LLP, Chandigarh, India
  • Prabhat Kumar  Institute of Reproduction and Child Cares and IVF Centre, Panchkula, Haryana, India
  • Kamla Singh  
  • Pulakes Purkait  

DOI:

https://doi.org//10.32628/IJSRST218483

Keywords:

Semen Analysis, Semen Freezing, Freezing Media, Semen Thawing, Sperm Motility and Storage in Liquid Nitrogen.

Abstract

Objective : To compare the rate of sperm survival and motility through semen freezing and thawing during infertility treatment. Methodology: In this bidirectional observational study, we enrolled 31 patients who underwent semen analysis for infertility treatment at the Institute of Reproduction and Child Cares & IRCC IVF Centre, Panchkula, Haryana, from June 2020 to December 2020. Out of these patients, 21 (67.74 %) were considered for semen freezing and thawing. For the rest of the ten patients (32.25 %), sperm count and motility were not good, and we excluded them from this study. Semen freezing based upon sperm count and motility were done. We did semen thawing after two weeks of semen freezing and recorded the sperm survival and motility. Results: Post thaw sperm survival rate and motility was 37.66% compare to pre-cryopreservation (61.82%). The observed rate of sperm motility declining was 24.16 % after cryopreservation/freezing. Conclusion: The present study results concluded that sperm's cryopreservation results in a decrease in sperm motility. There is a need of finding more accurate and reliable methods to freeze and thaw semen.

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International Journal of Scientific Research in Science and Technology

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Published

2021-08-30

Issue

Volume 8, Issue 4, July-August-2021

Section

Research Articles

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How to Cite

[1]
Ram Dayal, Sumer Singh, Sunil K Thakur, Prabhat Kumar, Kamla Singh, Pulakes Purkait, " Effect of Semen Freezing and Thawing on Sperm Survival And Motility Rate : A Comparative Analysis, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 8, Issue 4, pp.511-517, July-August-2021. Available at doi : https://doi.org/10.32628/IJSRST218483