Development of Nano Biosensor for Cholesterol Detection

Authors

  • Abinayashree Nagaraj  Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
  • Harshini Murugesan  Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
  • Lakshmi Subash  Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
  • Libin Johnson  Student Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India
  • Baalakumar  Assistant Professor Department of Biomedical Engineering, Velalar College of Engineering and Technology, Erode, Tamil Nadu, India

Keywords:

Cholesterol, Enzyme, Silver Nanoparticles, rGO.

Abstract

The aim of this study is to develop cholesterol biosensor using synthesized reduced Graphene Oxide (rGO) by modified Hummer’s method and reduced Graphene Oxide-Silver (rGO-Ag) nanocomposite had prepared by a simple chemical method. The rGO-Ag nanocomposite was synthesized in the presence of Silver Nitrate (AgNo3) and Sodium borohydride (NaBH4). The synthesized reduced Graphene Oxide (rGO) and reduced Graphene Oxide–silver rGO-Ag nanocomposite were characterized by Ultraviolet-Visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR) and Dynamic Light Scattering (DLS). Then the Cholesterol Oxidase (ChOx) enzyme is to be immobilized on rGO-Ag poly-pyrrole network. The rGO-Ag poly-pyrrole/ChOx enzyme is coated on electrode and its electrochemical studies are to be accomplished. The bioenzyme integrated nanostructure platform is very sensitive toward cholesterol and it has a fast response time.

References

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International Journal of Scientific Research in Science and Technology

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Published

2020-03-05

Issue

Volume 5, Issue 5, March-April-2020

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Abinayashree Nagaraj, Harshini Murugesan, Lakshmi Subash, Libin Johnson, Baalakumar "Development of Nano Biosensor for Cholesterol Detection" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 5, Issue 5, pp.223-226, March-April-2020.