Extraction and Detection of Zeatin from Moringa Oleifera Leaves and to Check its Crude Extract Effect on Plant Growth

Authors

  • Jyoti Raju Kadam  Department of Biotechnology, Mahatma Education Society’s, Pillai College of Arts, Commerce & Science, New Panvel, Navi Mumbai, Maharashtra, India
  • Tanmay D. Joshi  Department of Biotechnology, Mahatma Education Society’s, Pillai College of Arts, Commerce & Science, New Panvel, Navi Mumbai, Maharashtra, India
  • Anushree L. Shinde  Department of Biotechnology, Mahatma Education Society’s, Pillai College of Arts, Commerce & Science, New Panvel, Navi Mumbai, Maharashtra, India
  • Raksheeta V. Shetty  Department of Biotechnology, Mahatma Education Society’s, Pillai College of Arts, Commerce & Science, New Panvel, Navi Mumbai, Maharashtra, India
  • Tanushri B. Rakshaskar  Department of Biotechnology, Mahatma Education Society’s, Pillai College of Arts, Commerce & Science, New Panvel, Navi Mumbai, Maharashtra, India

Keywords:

Zeatin, Moringa oleifera, methanolic extract, Retardation factor, Thin Layer Chromatography (TLC), Brassica nigra

Abstract

In this study, plant growth hormone zeatin (cytokinin) in Moringa oleifera leaves was extracted and its detection was done. 80% methanolic extract was prepared from leaves of Moringa oleifera (drumstick plant) for the extraction and detection of zeatin (cytokinin). The methanolic extract was further partitioned into Zeatin (cytokinin) fraction using methyl acetate and water saturated n-butanol. The presence of plant growth hormone zeatin in the extracted sample from Moringa oleifera leaves was identified and detected by calculating the Retardation factor (Rf) value using the Thin layer chromatography (TLC) technique. The effect of crude extract of Moringa oleifera leaves was checked on Brassica nigra (black mustard) growth.

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International Journal of Scientific Research in Science and Technology

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Published

2018-07-30

Issue

Volume 4, Issue 9, July-August-2018

Section

Research Articles

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How to Cite

[1]
Jyoti Raju Kadam, Tanmay D. Joshi, Anushree L. Shinde, Raksheeta V. Shetty, Tanushri B. Rakshaskar, " Extraction and Detection of Zeatin from Moringa Oleifera Leaves and to Check its Crude Extract Effect on Plant Growth, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 9, pp.76-79, July-August-2018.