Isolation and Application of Diazotrophic Bacteria as Microbial Inoculants for Sugarcane Crops for Drought Tolerance Nature

Abstract

Sugarcane, a critical commodity in India, necessitates technical advances in production efficiency to enhance the country's agricultural energy balance. This research included four replications and a total of 24 plots.In this experiment, analytical-grade chemicals and double-distilled water were used throughout the procedure. Each soil sample was tested for its texture, pH, EC, organic carbon content, and phosphorus availability, among other characteristics, before being analysed. Each site had 20-30 cores of 2.5 cm diameter x 15-20 cm length extracted from the sugarcane root rhizosphere soil, with the diameter and length varying from site to site. For the roots and the surrounding earth, we dug 15-20 cm deep into the ground. After being transferred to polythene bags and kept at 37°C overnight, the samples were used to isolate AM fungal spores. In order to statistically analyse the data obtained from diverse features, the RBD design was used.