Identification of Cytotoxicity of Marine Sponge Dendrilla Membranosa Against L929 Cell Line

Authors

  • Joicy Abraham  Research Scholar, Interdisciplinary Research Centre, Department of Biotechnology, MS University, Tirunelveli, India
  • Mahija S. P  Research Scholar, Interdisciplinary Research Centre, Department of Biotechnology, MS University, Tirunelveli, India
  • Jency George  Research Scholar, Interdisciplinary Research Centre, Department of Biotechnology, MS University, Tirunelveli, India
  • Dr. Manjusha W. A  Head and Assistant Professor of Biotechnology Department, Malankara Catholic College, MS University, Tirunelveli, India

DOI:

https://doi.org//10.32628/IJSRST1841092

Keywords:

Dendrilla Membranosa, GC-MS, Anticancer Activity, Antimicrobial Activity, Antifungal Activity

Abstract

Marine sponges are rich sources of pharmacological active compound. Marine sponge, Dendrilla membranosa was collected from the Vizhingam coast. The sponge extract was tested against eight human bacterial pathogens. The bioactive compounds present in marine sponge were determined by GC-MS analysis. The cytotoxic effect of the sponge was evaluated by using MTT assay. The extracts showed potent anti-bacterial activity against Staphylococcus aureus, Bacillussubtilis, Streptococcus pyogens. The bioactive compound present in marine sponge was identified by GC-MS analysis and the compounds are ethane, butane, formate pentane, Alpha d-galactopyranoside, 2-Ethylhexyl2-ethylhexanoate, Hexahydro hexitol, Styrene, Alpha-d glucopyranoside 1-pentyl-2-proppyl-1-octene, nonadecane, 1,2-benzenedicarboxylic acid, bis-2, Octanoic acid. The test material showed none cytotoxic response to fibroblasts cells. The results of present investigation revealed that, Dendrilla membranosa is a potential source of novel anticancer and antibacterial leads.

References

  1. Meg Dowell (2018). Truly Dreadful Things Cancer Does to Your Body. The cheat sheet. 2018.
  2. Bergquist and Bedford (1978) P.R. The incidence of antibacterial activity in marine Desmospongie, Systemic and Geographic consideration. Mar.Biol. 1978; 46:215-221.
  3. Blunt et al (2005) Marine natural products. Nat. Prod. Rep. 2005; 22:15-61.
  4. Braekman et al (1992) Chemotaxonomy of Agelas porifera: Desmospongiae. Biochem Syst Ecol.1992; 2095: 417-31.
  5. Cappuccino and Sherman (2004) Microbiology, A Laboratory Manual; 2004.
  6. Connor (1982) CRC Hand Book of Chemotherapeutic Agents; 1982: pp(1): 219-259.
  7. Azevedo et al (2008) Investigation of the anti-inflammatory and analgesic effects from an extract of Aplysina caissara, a marine sponge, Fundament. Clinical Pharmaco.2008; 22(5):549-556.
  8. Dai et al (2006) Triterpenes from a South African species of the marine sponge Axinella inhibit hypoxia-inducible factor-1 (HIF-1) activation in both breast and prostate tumor cells. J Nat Prod. 2006; 69:1715-20.
  9. Kobayashi et al (1991) Purealidin a new cytotoxic bromotyrosine-derived alkaloid from the Okinawan marine sponge Psammaplysilla purea. Experientia.1991;47:299-300.
  10. Urban et al (1999) Axinellamines A-D, novel imidazo-azolo-imidazole alkaloids from the Australian marine sponge Axinella sp. J Org Chem.1999;64:731-5.
  11. Bharat et al (2008) Antibacterial activity of marine sponges from rocky reefs of southwest coast of India.Journal of Basic and Applied Biology.2008;2(1), 87-93.
  12. Pettit et al.(2004) Antineoplastic agents 430.Isolation and structure of cribrostatins 3, 4, and 5 from the Republic of Maldives Cribrochalina species. J Nat Prod.2004; 63:93-798

International Journal of Scientific Research in Science and Technology

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Published

2019-01-30

Issue

Volume 6, Issue 1, January-February-2019

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Joicy Abraham, Mahija S. P, Jency George, Dr. Manjusha W. A, " Identification of Cytotoxicity of Marine Sponge Dendrilla Membranosa Against L929 Cell Line, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 6, Issue 1, pp.147-152, January-February-2019. Available at doi : https://doi.org/10.32628/IJSRST1841092