Treating the leaves of mulberry, Morus alba (L) with aqueous solution of seed powder of cowpea, Vigna unguiculata (L) and feeding fifth instar larvae of silkworm, Bombyx mori (L) for the fortification of the cocoon and silk filament

Authors

  • Manisha Mahendra Nalwade  Shardabai Pawar Mahila Mahavidyalay, Tal-Baramati, Dist-Pune, Maharastra, India
  • Kajal Appasaheb Pondkule  Shardabai Pawar Mahila Mahavidyalay, Tal-Baramati, Dist-Pune, Maharastra, India
  • Trupti Nandkumar Gaikwad  Shardabai Pawar Mahila Mahavidyalay, Tal-Baramati, Dist-Pune, Maharastra, India
  • Vitthalrao Bhimasha Khyade  Sericulture Unit, Malegaon Sheti Farm, Agricultural Development Trust Baramati, Shardanagar, (Malegaon Khurd) Post Box No.- 35 ,Tal. Baramati, Dist. Pune, Maharashtra, India

Keywords:

Bombyx Mori L., Digestive Enzymes, Midgut, Morus Alba L, Vigna Unguiculata

Abstract

The cowpea (Vigna unguiculata) seed powder was dissolved in distilled water and diluted to 2.5%, 5%, 7.5%, and 10% concentrations. Fresh mulberry leaves were dipped in each concentration of aqueous solution of cowpea seed powder for half an hour. 1000 ml solution was used for 100 grams of mulberry leaves. Treated mulberry leaves were drained off completely and then used for feeding. The mulberry leaves were fed five times per day at the rate of 100 grams per 100 larvae for each time. Untreated group of larvae were feed with untreated mulberry leaves. Water treated group of larvae were feed with water treated mulberry leaves. The experimental groups of larvae were feed with feed separately with 2.5 percent cowpea treated; 5.00 percent cowpea treated; 7.5 percent cowpea treated and 10.00 percent cowpea treated mulberry leaves. Treating the mulberry leaves with various concentrations of aqueous solutions of cowpea seed powder and feeding to the fifth instar larvae of multivoltine cross breed race of silkworm, Bombyx mori (L) was found reflected into significant improvement in the weight of cocoon (31.862 Percentages); cocoon shell weight (52.336 percentages); pupal weight (26.336 percentages) and shell ratio. There was significant improvement in the silk filament length ( 00.323 percentages); silk filament weight ( 21.317 percentages) and denier scale of silk filament. Midgut enzymes (Protease, Amylase, Trehalase, Sucrase and Urease) were found influenced through treating mulberry leaves with cowpea seed powder and feeding to the fifth instar larvae of multivoltine cross breed race of silkworm, Bombyx mori (L) (Race: PM x CSR2). The contents of cowpea seeds may be associated with improvement in the growth and development through increased pattern of protein turn over and overall metabolism.

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International Journal of Scientific Research in Science and Technology

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2018-02-28

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Volume 4, Issue 2, January-February-2018

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[1]
Manisha Mahendra Nalwade, Kajal Appasaheb Pondkule, Trupti Nandkumar Gaikwad, Vitthalrao Bhimasha Khyade, " Treating the leaves of mulberry, Morus alba (L) with aqueous solution of seed powder of cowpea, Vigna unguiculata (L) and feeding fifth instar larvae of silkworm, Bombyx mori (L) for the fortification of the cocoon and silk filament, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.1277-1288, January-February-2018.