Assessment of Anti-inflammatory Activity of Methanolic Extract of Ficus Bengalensis and Its Fractions in Rats

Authors

  • Jagdish R. Baheti  Kamla Nehru College of Pharmacy, Butibori, Nagpur, Maharashtra, India

Keywords:

F. bengalensis, anti-inflammatory, carrageenan.

Abstract

The present study was aimed to evaluate anti-inflammatory activity of methanolic extract of Ficus bengalensis and its fractions against carrageenan and formalin induced inflammation and oedema models in rats. Methanolic extract was prepared and it was fractionated using different solvent in increasing order of polarity such as toluene, ethyl acetate, chloroform and butanol and subjected to anti-inflammatory study. Degree of inflammation was measured plethysmometrically and compared with control and standard drug, diclofenac10mg/kg. All the drugs were administered orally. Treatment of rats with carrageenan and/ or formalin produced a significant inflammation and oedema. Rats pretreated with methanolic extract of bark of Ficus bengalensis 100-200 mg/kg body weight its ethyl acetate fraction exhibited significant reduction in inflammation in both models studied. The results obtained with methanolic extract of bark of F. bengalensis and its ethyl acetate fraction [FB1] was comparable with the standard anti-inflammatory agent diclofenac sodium. Maximum anti-inflammatory effect was found to be at the dose of 150mg/kg in case of methanolic extract and 50mg/kg in case of FB1 in both the models studied in rats. Our data suggest that F. bengalensis bark possesses a potential anti-inflammatory activity.

References

  1. The Wealth of India. Raw materials, Vol IV, F-G CSIR, Ministry of Natural Resources and Scientific Research: New Delhi 1987; 24-25.
  2. Augasti KT. Hypoglycemic action of bengalenoside, a glucoside isolated from Ficus bengalensis Linn. in normal and alloxan diabetic rabbits. Indian. J. Physiol. Pharmacol. 1975; 4: 218-220.
  3. Augusti KT, Daniel RS, Cherian S, Sheela CG, and Nair CR. Effect of leucopelargonin derivative from Ficus bengalensis Linn. on diabetic dogs,. Indian J. Med. Res. 1994; 99: 82-86.
  4. Kumar KR and Augasti KT. Antidiabetic effect of leucocyanidin derivative isolated from bark of Ficus bengalensis Linn. Indian. J. Biochem. Biophys. 1989:6 400-404.
  5. Daniel RS, Mathew BC, Devi KS. and Augasti KT. Antioxidant effect of two flavonoids from the bark of Ficus bengalensis Linn. in hyperlipidemic rats. Indian J. Expt. Biol. 1998; 36: 902-906.
  6. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C and Altinier G. A Screening of the topical Anti-inflammatory activity of some Central American plants. J Ethanopharmacol 2002; 8:211-215.
  7. Karl F Swingle: Evaluation for anti-inflammatory activity. In anti-inflammatory agents, chemistry and pharmacology, Vol.13-II, edited by Robert A. Scherrer and Michael W. White House, Academic Press, New York, San Francisco, London 1974: 34.
  8. Kundakovic T, Fokialakis N, Dobric S, Pratsinis H, Kletsas D, Kovacevic N and Chinou I. Evaluation of the anti-inflammatory and cytotoxic activities of naphthazarine derivatives from Onosma leptantha. Phytomedicine. 2006; 13: 290–294.
  9. Harborne JB. In: Phytochemical methods. Chapman and Hall Ltd: London; 1973; 52-105.
  10. Winter CA, Risley EA and Huss GW. Carrageenin induced oedema in hind paw of the rats as an assay for antiinflammatory drugs. 1962; Proc. Soc. Exp. Biol. Med. 111: 544-545.
  11. Parmar NS, Ghosh MN. Antiiflammatory drugs : mechanistic approach Indian J. Pharmacol. 1978 ; 10: 277-278. 
  12. Chau TT. Analgesic testing in animal models. In: Pharmacologicalmethods in the control of inflammation. Alan R Liss Inc. 1989 195-212.
  13. Baheti JR, Awati ST. et al,. Anti-Inflammatory Herbs: A Review. Deccan J. Natural Products 2011; 2:1-15.
  14. Larsen GL and Henson PM. Mediators of inflammation. Annu. Rev. Immuno. 1983 ; 1: 335-346.
  15. Broooks PM and Day RO. Nonsteriodal anti-inflammatory drugs : differences and similarities. N. Engl. J. Med. 1991 324: 1716-1723.
  16. Vane J and Booting R. Mode of action of antiinflammatory drugs. FASEB J. 1990 ; 1: 89-95.
  17. Giulia Di Carlo, Nicola M, Angelo A Izzo and Francesco Capasso. Life Sci. 1979 ; 65: 337-345.
  18. Conner EM and Grisham MB. Inflammation, free radicals and antioxidants. Nutrition. 1996 ; 12: 274-287.
  19. Winrow VR, Winyard PG, Morris CJ, Blake DR. Free radicals in inflammation : second messangers and mediators in tissue destruction. Br. Med. Bull. 1993 ; 49: 506-511.
  20. Greenwald RA. Animal models for evaluation of arthritic drugs. Method Find Clin Pharmacol 1991; 13:75-83.
  21. Banerjee S, Sur TK, Mandal S, Chandra Das P, Sikdar S. Assessment of the anti-inflammatory effects of Swertia chirata in acute and chronic experimental models in male albino rats. Indian J Pharmacol 2000; 32:21-24.
  22. Larson R. A, ‘Antioxidants from higher plants’, Phytochemistry, Vol.27, 1988; 969-978.

International Journal of Scientific Research in Science and Technology

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Published

2018-01-30

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Volume 4, Issue 2, January-February-2018

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Research Articles

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How to Cite

[1]
Jagdish R. Baheti, " Assessment of Anti-inflammatory Activity of Methanolic Extract of Ficus Bengalensis and Its Fractions in Rats, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 2, pp.2099-2103, January-February-2018.