Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Tizanidine Hydrochloride in Bulk and Tablet Formulation

Authors

  • Sonali D Labhade  SJVPM's RMD Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India
  • Sanjay R Chaudhari  SJVPM's RMD Institute of Pharmaceutical Education and Research, Pune, Maharashtra, India
  • Ravi B Saudagar  KCT's, R G Sapkal College of Pharmacy, Nashik, Maharashtra, India

Keywords:

Diclofenac Sodium, Tizanidine Hydrochloride, High Performance Liquid Chromatography.

Abstract

A reverse phase high performance liquid chromatographic method (RP-HPLC) hasbeen developed for the simultaneous estimation of Diclofenac sodium (DICS), and Tizanidine Hydrochloride (TIZH) in the pharmaceutical formulation. The chromatographic column used was a reverse phase 4.6250 mm Hypersil C18 HPLC column with 5m (particles) packing. The mobile phase was ACN: Phosphate buffer pH 7.0 (50:50 v/v) delivered at a flow rate of 1.0 mL/min. The injection volume was 25 L. Elute was analyzed by a UV detector set at 220 nm .Linearity was obtained inthe concentration range of 2-10 μg/ml for DICS, 2.5- 12.5 μg/ml Tizanidine hydrochloride. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed RP-HPLC method may be used for determining Diclofenac Sodium and Tizanidine Hydrochloride in bulk drug samples.

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International Journal of Scientific Research in Science and Technology

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Published

2018-02-28

Issue

Volume 4, Issue 2, January-February-2018

Section

Research Articles

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How to Cite

[1]
Sonali D Labhade, Sanjay R Chaudhari, Ravi B Saudagar "Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Sodium and Tizanidine Hydrochloride in Bulk and Tablet Formulation" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 4, Issue 2, pp.1413-1417, January-February-2018.