Development of an Effective Microbial Consortium for Greywater Treatment

Authors

  • Singh. A  VES College of Arts, Science and Commerce, Chembur, Mumbai, Maharashtra, India
  • Nair. S. S  VES College of Arts, Science and Commerce, Chembur, Mumbai, Maharashtra, India
  • Mehetre. S. T.  NABTD, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India

Keywords:

CETP, STP, BOD, TSS, Bacterial Seed

Abstract

Greywater consists of used water from showers, baths, wash basins, washing machines and kitchen sinks and excludes black water (urine and sewage flush water). About 60-70% of the total domestic wastewater generated comprises greywater. In modern times with an exploding population and limited water resources, effective water management is a challenge for civic authorities and greywater recycling can substitute drinking water usage for purposes like toilet flush, gardening etc. Microorganisms have the potential to degrade organic and inorganic pollutants and are used for such purposes in STP’s and CETP’s globally. The present study deals with the development of a microbial consortium capable of treating kitchen sink greywater collected from institutional canteen with the objective of development of a novel and economic system for its treatment. For this purpose 13 isolates were tested for their potential of treating such water and 6 isolates were developed into a consortium referred to as Bacterial Seed (BS). The treatment process consisted of 50 mg/L BS and 1% KMnO4 with aeration. These levels were subsequently brought down to 5mg/L BS and 0.01% KMnO4. Treated water was tested for BOD and total suspended solids (TSS) reduction at ……. days and achieved 92.5% and 75% reduction in these values which met the national and international guidelines for reuse. Further bacteria were characterized and 16S rRNA sequencing for one isolate was done. The developed consortium can be implemented for large scale greywater treatment with the huge potentia for non-potable reuse within communities.

References

  1. Abdel-Shafy, Hussein & Makki, Ahmed & Mohamed-Mansour, Mona. (2014). Greywater treatment via hybrid integrated systems for unrestricted reuse in Egypt. Journal of Water Process Engineering. 1. 101-107. 10.1016/j.jwpe.2014.04.001.
  2. Abhishek Waghmare,? 2016, On World Water Day, Worst Water Crisis In Decade, Indiaspend
  3. Ajay Jindal & Shama Kamat (2017), Water Recycling and Reuse for Domestic and Industrial Sectors, Chemtech Foundation India
  4. Amrita Saha, Subhas Chandra Santra, (2014), 'Isolation and Characterization of Bacteria Isolated from Municipal Solid Waste for Production of Industrial Enzymes and Waste Degradation', Journal of Microbiology & Experimentation, Volume 1 Issue 1.
  5. Anushka Devale, Priyanka Patil, Shreya Sabale, (2015), 'Isolation and Characterization of Protease Producing Bacteria from Rhizosphere Soil and Optimization of Protease Production Parameters', International Journal of Current Microbiology and Applied Science, Special Issue-2: 58-64.?
  6. B. B. Kaliwal, Basavaraj I. Patagundi, C.T. Shivasharan, (2014), 'Isolation and Characterization of Cellulase producing bacteria from Soil', International Journal of Current Microbiology and Applied Science, 3(5): 59-69.
  7. E. Venkata, Naga Raju, G. Divakar, (2013), 'Screening and Isolation of Pectinase producing Bacteria from Various Regions in Bangalore', International Journal of Research in Pharmaceutical and Biomedical Sciences, Vol. 4.
  8. Environment (Protection) Rules (EPA) 1986 Ministry of Environment and Forests (Department of Environment, Forest and Wildlife), New Delhi, India.
  9. Ghaitidak Dilip & Yadav Kunwar. (2013). Characteristics and treatment of greywater-A review. Environmental science and pollution research international. 10.1007/s11356-013-1533-0.
  10. Greywater recycling & Reuse. Egyptian -German Private Sector Development Program (PSDP).
  11. IS 3025 (Part 44):1993 METHODS OF SAMPLING AND TEST (PHYSICAL AND CHEMICAL) FOR WATER AND WASTE WATER. PART 44 FOR BIOCHEMICAL OXYGEN DEMAND
  12. IS 3025 (PART 17): 1984 METHODS OF SAMPLING AND TEST (PHYSICAL AND CHEMICAL) FOR WATER AND WASTE WATER. PART 17 NON-FILTERABLE RESIDUE (TOTAL SUSPENDED SOLIDS).
  13. IS 3025 (PART 38): 1989 METHODS OF SAMPLING AND TEST (PHYSICAL AND? CHEMICAL) FOR WATER AND WASTE WATER. PART 38 DISSOLVED OXYGEN.
  14. John R. Buchanan & Robert W. Seabloom, (2004) Aerobic treatment of wastewater & Aerobic treatment units, University Curriculum Development for Decentralized Wastewater Management.???????????????????
  15. LAMBE, JS, and RS CHOUGULE. Greywater-Treatment and Reuse." IOSR Journal of Mechanical and Civil Engineering (IOSR-JMC1684, PP: 20 26.
  16. L Hernandez Leal, L. H., Zeeman, G., Temmink,(2007). Characterization and biological treatment of greywater. Water Science and Technology.
  17. Mehta, Prashant. (2017). Impending water crisis in India and comparing clean water standards among developing and developed nations,? Scholars Research Library.4 (1):497-507
  18. M. Lakshmi Narasu, E. Sirisha, N. Rajasekar, (2010), 'Isolation and Optimization of Lipase Producing Bacteria from Oil Contaminated Soils', Advances in Biological Research,4 (5): 249-252.
  19. Sasmita Mishra, Niranjan Behera, (2008), ‘Amylase Activity of a Starch Degrading Bacteria Isolated from Soil receiving Kitchen Wastes’, African Journal of Biotechnology Vol. 7 (18), pp. 3326-3331,ISSN 1684-53152008 Academic Journals.
  20. Singh, A.R., Nair, S. S., Mehetre, S. T. and Mukhopadhyaya, R. M. (2017) Bacillus gottheilii strain VESASC1 16S ribosomal RNA gene, partial sequence. GenBank accession No. KY491643

Downloads

Published

2018-04-30

Issue

Section

Research Articles

How to Cite

[1]
Singh. A, Nair. S. S, Mehetre. S. T., " Development of an Effective Microbial Consortium for Greywater Treatment, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 5, pp.96-103, March-April-2018.