Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum

Authors

  • Rohini C. Yevale  Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India
  • Sushma G. Sabharwal  Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University Pune, Maharashtra, India

Keywords:

Peroxidase, Macrotyloma Uniflorum, Seedlings, Hydrogen Peroxide.

Abstract

Peroxidases are the heme containing enzymes that catalyse oxidation of substrates like phenolic compounds by reducing hydrogen peroxide concurrently. They play a key role in a number of metabolic processes such as regulation of cell elongation, lignification of cell wall, phenolic oxidation and defence against stress. Peroxidase was isolated from 4 days old seedlings of Macrotyloma uniflorum followed by germination study. The enzyme showed optimum pH 6 and optimum temperature 30°C. Peroxidase showed a stability over a broad pH range (4 to 9). Enzyme also was found to be stable up to 70°C. Kinetic studies were also performed.

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International Journal of Scientific Research in Science and Technology

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Published

2018-01-20

Issue

Volume 5, Issue 1, January-February-2018

Section

Research Articles

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How to Cite

[1]
Rohini C. Yevale, Sushma G. Sabharwal "Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 5, Issue 1, pp.208-213, January-February-2018.