Biochemical Studies on Peroxidase from the Seeds of Macrotyloma uniflorum
Keywords:
Peroxidase, Macrotyloma Uniflorum, Seedlings, Hydrogen Peroxide.Abstract
Peroxidases are the heme containing enzymes that catalyse oxidation of substrates like phenolic compounds by reducing hydrogen peroxide concurrently. They play a key role in a number of metabolic processes such as regulation of cell elongation, lignification of cell wall, phenolic oxidation and defence against stress. Peroxidase was isolated from 4 days old seedlings of Macrotyloma uniflorum followed by germination study. The enzyme showed optimum pH 6 and optimum temperature 30°C. Peroxidase showed a stability over a broad pH range (4 to 9). Enzyme also was found to be stable up to 70°C. Kinetic studies were also performed.
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