Exploring Anti-Inflammatory Potential in Leaves of Jamun (Syzygium Cumini)

Authors

  • Pooja Patil  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India
  • Kanchan Magar  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India, Pravara Institute of Medical Sciences (DU), Loni (Bk), Tal. Rahata, Dist. Ahmednagar, Maharashtra, India
  • Twinkle Bansode  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India
  • Amit Gupta  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India
  • Sushma Chaphalkar  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India
  • Ashok Patil  Vidya Prathishtan’s School of Biotechnology (VSBT), Baramati, Maharashtra, India
  • Anant Sherkhane  

Keywords:

Herbal preparation, Bioactive compounds, S. Cumini, TLC, Aspirin, Diclofenac sodium

Abstract

Inflammation is initiated as healing process by the tissue in response to injury by pathogens, irritants or cell damage. Anti-inflammatory drugs used today to cure the disease have serious side-effects. The focus of research is to study the bioactive compounds from medicinal plants with anti-inflammatory property. The work is carried out with Syzygium cumini leaves (family-Myrtaceae). Leaves are reported to posses anti-inflammatory activity in crude extract of leaves and bark. In our study, ethanolic extract of leaves was screened for the anti-inflamatory activity and bioactive compound was partially purified by Thin Layer Chromatography (TLC) technique. Partially purified bioactive compounds were further analysed using various in vitro models such as inhibition of albumin denaturation assay and HRBC membrane stabilization assay. Aspirin and diclofenac sodium were used as standard drug. It is cleared from the result that the bioactive compound tannins at concentration of 100?g/ml posses 99.50% inhibition of heat induced protein denaturation as compared with the standard drug Aspirin i.e. 89.26%. In HRBC membrane stabilization activity tannins at concentration of 1mg/ml showed 82.94% protection of HRBC membrane while the standard diclofenac sodium showed 70.41% protection. Overall study claims that ethanol extract of S. cumini leaves posses potential anti-inflamatory activity due. Further study is required to purify and identify the specific bioactive compound.

References

  1. Vashishtha, V., Sharma G. N., Gaur M. and R. Bairwa. 2014. A review on some plants having anti-inflammatory activity. J. Phytopharmacology, 3(3): 214-221
  2. Padmanabhan, P. and S. N. Jangle. 2012. Evaluation of in-vitro anti-inflammatory activity of herbal preparation, a combination of four medicinal plants. Intl. J. Basic and Applied Medil Sci., 2(1):109 -116
  3. Sudevan, S., Sundar, S., Ranganayaki, P., Aswathy, G., Shafina, J., and V. Ramasamy. 2015. Studies on in-vitro Anti-inflammatory activity of Acmella oleracea metabolic compounds. JCPS 8(2): 974-2115
  4. Bhagyasri, Y., Lavakumar, V., Divya Sree. M. and C. Ashok Kumar.2015. An overview on anti-inflammatory activity of indian herbal plants. Intl. J. Res. Pharmaceutical and Nano Sci. 4(1): 1- 9
  5. Leelaprakash, G. and S. Dass. 2011 In-vitro anti-inflammatory activity of methanol extract of enicostemma axillare. Int. J. Drug Dev. & Res. 3(3): 189-196
  6. Maroon, J. C., Bost, J. W., & Maroon, A. 2010. Natural anti-inflammatory agents for pain relief. Surgical Neurology International, 1, 80. http://doi.org/10.4103/2152-7806.73804
  7. Swami, S., Thakor, N., Patil, M. and P. Haldankar . 2012. Jamun (Syzygium cumini (L.): A Review of Its Food and Medicinal Uses. Food and Nutrition Sci. 3: 1100-1117
  8. Bansode, T. S., A. Gupta and B. K. Salalkar. 2016. In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels. Plant Science Today 3(4): 360-367. http://dx.doi.org/10.14719/pst.2016.3.4.26 4
  9. Bansode, T. S., and B. K. Salalkar. 2017. Phytotherapy: Herbal medicine in the management of Diabetes mellitus. Plant Science Today. 4(4):161-165. doi:10.14719/pst.2017.4.4.347
  10. Yadav, D., Lalit, A., Singh, S., Galgut, J. and M. Beg. 2013. Evalution of antidiabetic and phytochemical activity of 50% methanolic extract of jamun seed (syzygium cumini). Search And Research. 4 (3): 13-16
  11. Subramanian, R., Subbramaniyan, P. and V. Raj.2012. Determination of some minerals and trace elements in two tropical medicinal plants. Asian Pacific J. Tropical Biomedicine. 555-558
  12. Bansode, T.S. and B.K Salalkar. 2015.Phytochemical analysis of some selected Indian medicinal plants. Int J Pharm Bio Sci.6(1):550-556.
  13. Biradar, V. 2016. Extraction of Phytochemicals from Local Selected Plants and their Antibacterial Role. Int.J.Curr.Microbiol.App.Sci. 5(11): 707-720
  14. Biradar, S. and B. Rachetti. 2013. Extraction of some secondary metabolites & thin layer chromatography from different parts of Centella asiatica L. (URB). American J. Life Sci. 1(6): 243-247
  15. Saha, R., Zaman, N. and P. Roy. 2013. Comparative evaluation of the medicinal activities of methanolic extract of seeds, fruit pulps and fresh juice of Syzygium cumini in vitro. J. Coastal Life Medicine. 1(4): 300-308
  16. Srividya And Chandra . 2015. In Vitro Anti-Inflammatory Activity Of Some Wild Fruits Of Karnataka. International Conference On Biological, Environment And Food Engineering. May 15-16
  17. Verma, A.,Kumar .A., Kavitha, D. And K.Anurag, Anti Denaturation and Antioxidant Activities of Annona cherimola In-Vitro. Intl. J. Pharma And Bio Sci. 2(2):0975-6299
  18. Chioma, A., Onyechi, O. and L. Ezeanyika. 2012. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J. Pharmaceutical Sci. 20:76
  19. Kambizi. L., Bakare-Odunola, M. And A. Oladiji. 2017. Proteinease Inhibition, Membrane Stabilization,Antioxidant And Phytochemical Evaluations Of Leaves, Seeds And Calyces Of Four Selected Edible Medicinal Plants. Cogent Chemistry. 3: 1314064
  20. Nile, S. H. and N. N.Khobragade. 2009. Determination of Nutritive Value and Mineral Elements of some Important Medicinal Plants from Western Part of India. J. Medicinal Plants. 8(5):80-88
  21. Merchant, S. S. 2010. The Elements of Plant Micronutrients. Plant Physiol . 154: 512–515.
  22. Banu, R. and N. Nagarajan. 2014. TLC and HPTLC fingerprinting of leaf extracts of Wedelia chinensis (Osbeck) Merrill. J. Pharmacognosy and Phytochemistry. 2(6): 29-33
  23. Dolowy, M. and A.Pyka.2014. TLC-Densitometric method for qualitative analysis of betamethasone and its related compounds in pharmacautical preparations. acta poloniae pharmaceutica n drug research.71(6): 922-932
  24. Ullah, H., Zaman, S., Juhara, F., Lucky Akter2, Tareq, S., Masum, E. and R. Bhattacharjee. 2014. Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract ofCurcuma zedoaria rhizome. BMC Complementary and Alternative Medicine. 14:346
  25. Chayen, J. and L. Bitensky. 1971. Lysosomal enzymes and inflammation. Ann. rheum. Dis. 30:522. Downloaded from http://ard.bmj.com/ on January 17, 2018 - Published by group.bmj.com.
  26. Patil, K. and C. Patil. 2017. Anti-inflammatory activity of bartogenic acid containing fraction offruits of Barringtonia racemosa Roxb. in acute and chronic animal models of inflammation. J. Traditional and Complementary Medicine. 7(1): 86-93

International Journal of Scientific Research in Science and Technology

Downloads

Published

2018-02-03

Issue

Volume 5, Issue 2, January-February-2018

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Pooja Patil, Kanchan Magar, Twinkle Bansode, Amit Gupta, Sushma Chaphalkar, Ashok Patil, Anant Sherkhane "Exploring Anti-Inflammatory Potential in Leaves of Jamun (Syzygium Cumini)" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 5, Issue 2, pp.90-96, January-February-2018.