Efficacy of Some Medicinal Plants on MDR Staphylococcus Saprophyticus Isolated from Urine sample

Authors

  • R. N. Jadhav  Department of Microbiology, Shivneri Mahavidyalaya, Shirur(A), Maharashtra, India

Keywords:

Antibacterial activity, Disc diffusion method, MDR, Medicinal plant extracts, Staphylococcus saprophyticus.

Abstract

Staphylococcus saprophyticus is Gram positive spherical shaped bacteria. It is opportunistic pathogen. It is a normal flora of skin and periurethral area. It can cause UTI particularly in sexually active young women.UTI is second most common type of infection in community practice. UTI refers to the presence of microorganism in the urinary tract including urinary bladder, prostate, collecting system or kidney. The syndrome ranges from asymptomatic bacteriuria to perinephrid abscess with sepsis. Due to indiscriminate use of antibiotics there is an increase in resistance among microorganisms. Bacteria become resistant to antibiotic by different ways. Patent suffering from antibiotic resistant strain fail to respond antibiotic treatment. So there is a continuous demand of new drug in the world. This problem of drug resistance among microorganism could overcome by drugs of plant origin. Due to this reason now a day the demand of herbal products as therapeutic agents is increasing all over the world. In the present study antibacterial activity of Aloe vera, Azadirachta indica & Citruslimon was studied against MDR Staphylococcus saprophyticus isolated from urine sample collected from civil hospital. Antibiotic susceptibility test of the clinical isolates of Staphylococcus saprophyticus was done by using modi?ed Kirby-Bauer disc diffusion method in accordance with the guidelines of the clinical & laboratory standards institute. Interpretation of resistance was based on the NCCLS criteria. The most common pattern of multiple drug resistance of isolates of Staphylococcus saprophyticus observed was erythromycin-nalidixic acid-penicillin-sulfamethoxazole-chloramphenicol. The antibacterial activity of solvent extract of plant was tested by agar well diffusion method. Citrus limon and Azadirachta indica showed moderate antibacterial activity while Aloe vera showed least antibacterial activity.

References

  1. Akhilesh K, Raghvendra P, Vikas S and Madhulika G. Antimicrobial property of lemon peel extract. Natl J Universal Pharm Life Sci 2012; 52:382-6
  2. Ananthanarayan and Paniker .Text book of Microbiology 9th edition ,University press (India) pvt Ltd, Hyderabad.2003;25: 199-207.
  3. Anderson J.D. The ecology of transferable drug resistance in Enterobacteria. Ann. Reu. Microbiol. 1968;22:131-180.
  4. Bauer A.W.,Kirby W.M. and Sherris J.C. Am. J. Clin. Pathol. 1966; 45:493.
  5. Biswas K, Chattopadhyay I, Banerjee RK and Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci. 2002; 82: 1336- 45.
  6. Cruickshank R., Duguid, J.P., Marmion B.P. and Swain R.H.A. Medical Microbiology. Chruchill Livingstone, Edinburgh London and New York. 1975.
  7. Dubey R.C & Maheshwari D. K. A Textbook of microbiology. S. Chand & Company Ltd. 2003; 106
  8. Dubey R.C & Maheshwari D.K. Practical Microbiology, S. Chand & company Ltd. 2014;296-309.
  9. Govindachari T R. Chemical and biological investigations on Azadirachta indica Curr. Sci. 1992; 63:117-122.
  10. Grindlay D. and A. Reynolds. J. Ethnopharma. 1986; 16: 7.
  11. Jager A.K., Hutching A.and Van Staden J. Screening Of Zulu Medicinal Plants for Prostaglandin Synthesis Inhibitors. J. Ethnopharmacol. 1996; 52(2): 95-100.
  12. Jain S.K. Medicinal plants, National Book Trust. 2003.
  13. Johnson C C: Defination, classification and clinical presence of urinary Tract infections. Med Clin North Am. 1991;75(2): 241-243.
  14. Kirtikar K.R. & Basu B.D. Indian Medicinal plants. Second edition. 1988.
  15. Kuroda M. Yamoshita A, Hirakawa H. Kumano M, Morikawa k et al. Whole Genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. Proc Natl Acad sci USA .2005; 102:13272-13277.
  16. Latham R H, Running K. Stamm W E. Urinary tract infection in young adult Women caused by Staphylococcus saprophyticus , JAMA.1983; 250:3063- 3066.
  17. Michael J. Pelzar, Jr. ECS Chain and Novel R Krieg. Microbiology- An Application Based Approach. 2010; 335, 682.
  18. Mishra A. et al. Antibacterial Effects of Crude Extract of A. indica against E. coli and S. aureus. Intl. J. of Sc, Envn and Tech. 2013 ; ( 2)5: 989 – 993.
  19. Nair R., Kalariya T. and Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk. J. Biol. 2005; 29: 41- 47.
  20. NCCLS. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. 10th ed. Approved standard M2-A10. Wayne, PA: Clinical and Laboratory Standards Institute. 2009.
  21. Pead L, Maskell R., Morris J. Staphylococcus saprophyticus as a urinary pathogen: 6 years prospective survey. Br Med J (Clin Res Ed) . 1985; 291:1157-1159.
  22. Vogler B K and Ernst E. Aloe vera: A systematic review of its clinical effectiveness. Br J of Gen Pract. 1994; 9:823-28.
  23. Widerstorm M, Wistron j , Sjostedt A , Monsen T . Coagulase negative staphylococci : Update on molecular epidemiology and clinical presentation with a focus on Staphylococcus epidermidis and Staphylococcus saprophyticus. Europian Journal of clinical microbiology and infectious diseases: Official publication of the Europian Society of Clinical Microbiology. 2012; 31(1):7-20.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2019-01-04

Issue

Volume 5, Issue 4, January-2019

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
R. N. Jadhav, " Efficacy of Some Medicinal Plants on MDR Staphylococcus Saprophyticus Isolated from Urine sample, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 5, Issue 4, pp.27-31, January-2019.