RP-HPLC Method Development and Validation for The Analysis of Pharmaceutical Drug - AXITINIB
Keywords:
AXITINIB, Method Development, Validation, 254nm, 3.0min.Abstract
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of AXITINIB. Isocratic elution at a flow rate of 1.2 ml /min was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Acetonitrile : 0.1M Acetic Acid 50:50 (v/v). The UV detection wavelength was at 254 nm. Linearity was observed in concentration range of 10-50 ppm. The retention time for AXITINIB was 3.0 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of AXITINIB.
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2019-07-30
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[1]
Ami R Patel, Dr. V G Patel "RP-HPLC Method Development and Validation for The Analysis of Pharmaceutical Drug - AXITINIB" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 6, Issue 4, pp.367-371, July-August-2019.