In silico Analysis and Molecular Modelling of NADH-cytochrome b5 Reductase 3 involved in Methemoglobinemia

Authors

  • Someshwar C. M. Moholkar  P.G. Research Center in Bioinformatics, Walchand Center for Biotechnology, Walchand college of Arts and Science, Solapur, Maharashtra, India
  • Mamta S. Kongari  P.G. Research Center in Bioinformatics, Walchand Center for Biotechnology, Walchand college of Arts and Science, Solapur, Maharashtra, India
  • Shubhangi S. Sakhare  P.G. Research Center in Bioinformatics, Walchand Center for Biotechnology, Walchand college of Arts and Science, Solapur, Maharashtra, India

Keywords:

Methemoglobinemia, Insilico, reductase, Nitrate, Random Coil, secondary structure.

Abstract

Methemoglobinemia is type of disease caused due to methemoglobin. Methemoglobin is type of hemoglobin in which the iron heme3+ is present instead of fe2+. Normally methemoglobin is present in every living cell, but if it is present in large amount then it will responsible for causing disease. Acquired methemoglobinemia known as acute methemoglobinemia caused by exposure of medicines, chemicals, foods. People suffering from genetic form have higher chances of developing acquired type. Most cases of acquired methemoglobinemia result from exposure to certain drugs or toxins. In the present study we have used different Insilico tools and techniques which are includes retrieval of NADH-cytochrome b5 reductase 3protein sequence from the UniProt KB database and physicochemical parameter analyzed by using Protoparam tool. In that Leucine had a maximum amino acid composition. The structure of a protein has a very important role in its function. The secondary structure was predicted by using SOPMA tool which indicated that the percentage of Random coils was higher than the percentage of alpha helix and extended strand. Then the 3D structure of b5 reductase 3 was predicted by using SWISS MODEL server and the model was validated by using PROCHECK analysis after validation of the model, the validation score was 94.7%.

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International Journal of Scientific Research in Science and Technology

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Published

2020-02-29

Issue

Volume 7, Issue 1, January-February-2020

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Someshwar C. M. Moholkar, Mamta S. Kongari, Shubhangi S. Sakhare, " In silico Analysis and Molecular Modelling of NADH-cytochrome b5 Reductase 3 involved in Methemoglobinemia, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 7, Issue 1, pp.229-233, January-February-2020.