Development of a Validated Stability Indicating RP-HPLC Method for Assay of Clopidogrel

Authors

  • Dr. D. S. Ghotekar  Department of Chemistry, N.V.P.Mandal's, Arts, Commerce and Science College Lasalgaon Tal-Niphad, Dist-Nashik, Maharastra, India
  • Vishal N. Kushare  Department of Pharmaceutics, Maratha Vidya Prasarak Samaja's Institute of Pharmaceutical Sciences, Adgaon, Nashik, Maharastra, India

Keywords:

Clopidogrel, Validation, RP-HPLC

Abstract

A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for estimation analysis of DRO. A Brownlee ODS C-18 column (250×4.6 mm i.d) chromatographic column equilibrated with mobile phase methanol-0.02 M KH2PO4 (80:20, v/v) (Final pH adjusted to 4 using Orthophosphoric acid) was used. Mobile phase flow rate was maintained at 1 ml/min and effluents were monitored at 254 nm. The sample was injected using a 20 ?l fixed loop, and the total run time was 9.946 min. Experimental conditions such as pH of mobile phase, column saturation time, selection of wavelength, etc. were critically studied and the optimum conditions were selected. In RP-HPLC linear range was found to be 1-10 ?g/ml and, mean recovery was found to be 99.65 and Rt of Clopidogrel was found to be 9.946 min.

References

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International Journal of Scientific Research in Science and Technology

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Published

2019-04-30

Issue

Volume 6, Issue 2, March-April-2019

Section

Research Articles

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Dr. D. S. Ghotekar, Vishal N. Kushare, " Development of a Validated Stability Indicating RP-HPLC Method for Assay of Clopidogrel, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 6, Issue 2, pp.834-838, March-April-2019.