Effect of Different Phyto-Hormones on Callogenesis in Gymnema sylvestre R. Br. : An Important Anti-Diabetic Plant

Authors

  • Bishnupriya Hansdah  Assistant Professor of Botany, North Orissa University, Takatpur, Baripada, Odisha, India
  • S. L. Sahoo  Professor of Botany, Utkal University, Vani Vihar, Bhubaneswar, India

Keywords:

Gymnema sylvestre R. Br., Diabetes, Callogenesis, Proliferation, Phytohormones.

Abstract

Gymnema sylvestre R. Br., belongs to the family Asclepiadaceae and commonly called as “Gudmar” is one of the popular medicinal plants acknowledged as a potent drug plant in Ayurveda as well as in homeopathic systems. This plant is mostly used medicinally in India and Southeast Asia for treatment of “sweet urine” or what we refer it as diabetes and also employed in the treatment of many other health problems. This study developed a novel cell culture system for in vitro callus induction, proliferation and growth of this species with improving the active principles in the plant. Nodal explants were inoculated in MS basal media, with supplementation of different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/l) of Auxins (2, 4-D and NAA) and Cytokinins (Kinetin and BAP). The explants were allowed to grow and the callus was maintained on the same medium for weekly analysis. The maximum callusing was obtained in the medium supplemented with combinations of Kn (2.5 mg/l) along with 2, 4-D (2.5 mg/l). The maximum fresh weight and dry weight was found when MS medium fortified with 2, 4-D (2.5 mg/l) followed by NAA at 2.5 mg/l. Among cytokinins, the Kinetin supplemented medium yield more callus then that of BAP at 2.0 mg/l. From the above study, it was quite conclusive that, the phytohormones play a significant role in regulation of the growth of callus and proliferation.

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International Journal of Scientific Research in Science and Technology

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Published

2018-03-30

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Volume 4, Issue 5, March-April-2018

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Research Articles

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[1]
Bishnupriya Hansdah, S. L. Sahoo, " Effect of Different Phyto-Hormones on Callogenesis in Gymnema sylvestre R. Br. : An Important Anti-Diabetic Plant, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 4, Issue 5, pp.1966-1972, March-April-2018.