Sero-Molecular Detection of Toxoplasma Gondii Infection among HIV-Positive Patients in Khartoum : A Cross Sectional Study

Authors

  • Elghazali Mohammed  Parasitology and Medical Entomology department, Medical Laboratory Sciences, El-Neelain University, Sudan
  • Mustafa Yassin  Parasitology and Medical Entomology department, Medical Laboratory Sciences, El-Neelain University, Sudan
  • Khalid Anan  Department of Virology, Central Laboratory, the Ministry of Higher Education and Scientific Research, Sudan
  • Awadalkareem Omer  Department of Virology, Central Laboratory, the Ministry of Higher Education and Scientific Research, Sudan
  • Mutaz A. Elsir  Department of Virology, Central Laboratory, the Ministry of Higher Education and Scientific Research, Sudan
  • Abdelrahim M. ElHussein  Department of Virology, Central Laboratory, the Ministry of Higher Education and Scientific Research, Sudan
  • Alamin Abdalkareem  Parasitology and Medical Entomology department, Medical Laboratory Sciences, El-Neelain University, Sudan
  • Osman Mansour  Parasitology and Medical Entomology department, Medical Laboratory Sciences, El-Neelain University, Sudan

DOI:

https://doi.org/10.32628/IJSRST218524

Keywords:

Abstract

Background and Aim : Toxoplasmosis is one of the most common comorbidities in HIV-positive patients with CD4+ T lymphocytes below 200 cells/μl. Toxoplasmosis with encephalitis may affect HIV infection, in particular in patients with developing AIDS. Early diagnosis and treatment of toxoplasmosis reduces the mortality rate in HIV-positive people. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection in HIV-positive patients in Khartoum, Sudan using serological and molecular methods. Methods : This was a descriptive cross sectional, hospital based study, blood sample were collected from 100 participants; out of them were 50 HIV/AIDS patients and 50 were healthy Blood donors attending HIV Center -Omdurman Hospital, and Sudan Heart Center blood bank respectively. Socio- demographic data were collected by structured questionnaire. Of the 50 HIV/AIDS patients, 25 (50%) were from each gender, their age ranged between 22 and 62 with mean of 39.5+10.69. They were classified into two age groups; from 22 to 42 years, and from 43 to 62 years, and their distribution was 29 (58%), and 21 (42%) respectively. According to the clinical stage they were classified into the four clinical-stage groups and their distribution was as following: 06 (12.0%) in stage 1, 02 (04%) in stage 2, 40 (80.0%) in stage 3, and 02 (04.0%) in stage 4. Twelve of them (22%) were under ART treatments. The 50 blood donors on their hand were all males, their age was ranged between 18 and 42 years old. Regarding their educatio, 15 (30%) were educated, while the remaining 35 (70%) were none educated. Mentioning their marital status 10 (20%) were married, while 40 (80%) were single. Regarding the contact with cats 25 (50%) has contact with cats. All samples collected were tested for anti-Toxoplasma IgG and IgM antibodies by ELISA, and by PCR for detection of Toxoplasma DNA.. Results: Out of the 50 HIV/AIDS patients; Anti-Toxoplasma IgM was detected in two patients (04%), while IgG was detected in 08 (16%) patients, the Toxoplasma DNA was detected in three (06%) patients.Regarding the blood donors group; all studied blood donors showed negative results for anti-toxoplasma IgM, while 16 (32%) showed positive anti-Toxoplasma IgG.No blood donors sample was detected positive for Toxoplasma DNA with PCR. There were no significant differences in comparison between the blood donors and the HIV/AIDS patients regarding serological and molecular toxoplasma test results. Conclusion : The current study showed a relatively high seroprevalance of anti-T.gondii IgG and low IgM antibodies in HIV-positive patients in comparison with previous studies in Sudan. Considering the relatively high seroprevalance rate of toxoplasma infection in blood donors reported in this study, toxoplasmosis should be considered as a significant transfusion risk.

References

  1. Wang ZD, Wang SC, Liu HH, Ma HY, Li ZY, Wei F, et al. Prevalence and burden of Toxoplasma gondii infection in HIV-infected people: a systematic review and meta-analysis. Lancet HIV. 2017;4(4):e177-e88.
  2. Fayer R, Dubey JP, Lindsay DS. Zoonotic protozoa: from land to sea. Trends Parasitol. 2004;20(11):531-6.
  3. Elsheikha HM, Azab MS, Abousamra NK, Rahbar MH, Elghannam DM, Raafat D. Seroprevalence of and risk factors for Toxoplasma gondii antibodies among asymptomatic blood donors in Egypt. Parasitol Res. 2009;104(6):1471-6.
  4. Studenicova C, Bencaiova G, Holkova R. Seroprevalence of Toxoplasma gondii antibodies in a healthy population from Slovakia. Eur J Intern Med. 2006;17(7):470-3.
  5. Malla N, Sengupta C, Dubey ML, Sud A, Dutta U. Antigenaemia and antibody response to Toxoplasma gondii in human immunodeficiency virus-infected patients. Br J Biomed Sci. 2005;62(1):19-23.
  6. Chemoh W, Sawangjaroen N, Siripaitoon P, Andiappan H, Hortiwakul T, Sermwittayawong N, et al. Toxoplasma gondii - Prevalence and Risk Factors in HIV-infected Patients from Songklanagarind Hospital, Southern Thailand. Front Microbiol. 2015;6:1304.
  7. Colombo FA, Vidal JE, Penalva de Oliveira AC, Hernandez AV, Bonasser-Filho F, Nogueira RS, et al. Diagnosis of cerebral toxoplasmosis in AIDS patients in Brazil: importance of molecular and immunological methods using peripheral blood samples. J Clin Microbiol. 2005;43(10):5044-7.
  8. Daryani A, Sharif M, Meigouni M. Seroprevalence of IgG and IgM anti-Toxoplasma antibodies in HIV/AIDS patients, northern Iran. Asian Pac J Trop Med. 2011;4(4):271-4.
  9. Okubo Y, Shinozaki M, Yoshizawa S, Nakayama H, Wakayama M, Hatori T, et al. Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffin-embedded tissue for polymerase chain reaction: a case report. J Med Case Rep. 2010;4:265.
  10. Julander I, Martin C, Lappalainen M, Guy E, Isberg B, Evengard B. Polymerase chain reaction for diagnosis of cerebral toxoplasmosis in cerebrospinal fluid in HIV-positive patients. Scand J Infect Dis. 2001;33(7):538-41.
  11. Siegel SE, Lunde MN, Gelderman AH, Halterman RH, Brown JA, Levine AS, et al. Transmission of toxoplasmosis by leukocyte transfusion. Blood. 1971;37(4):388-94.
  12. Addebbous A, Adarmouch L, TaliA et al. IgG anti-toxoplasma antibodies among asymptomatic HIV-infected patients in Marrakesh- Morocco. Acta Trop. 2012;123(1):49–52.
  13. Stuart E Siegel, Milford N. Lunde et al. Transmission of toxoplasmosis by leukocyte transfusion. Blood. 1971; 37(4) : 388- 394.
  14. Yazar S, Eser B & Yay M. Prevalence of anti- Toxoplasma gondii antibodies in Turkish blood donors. Ethiop Med J 2006; 44: 257–261.
  15. Ahmadpour E, DaryaniA, Sharif M et al. Toxoplasmosis in immuno-compromised patients in Iran: A systematic review and meta-analysis. J Infect Dev Ctries. 2014;8(12): 1503-1510.
  16. Walle F, Kebede N, Tsegaye A et al. seroprevalence and risk factors for toxoplasmosis in HIV infected and non-infected individuals in Bahir Dar, northwest Ethiopia. Parasite vector.2013 ;(1):15.
  17. Svobodová V, Literák I. Prevalence of IgM and IgG antibodies to Toxoplasma gondii in blood donors at the Czech republic. Eur J Epidemiol. 1998;14(8):803–805. doi: 10.1023/A:1007589422080.
  18. Alvarado-Esquivel C, Mercado-Suarez FM, Rodringuez-Briones A, Fallad-Torres L, Ayala-Ayala OJ, Nevarez-Piedra JL, Duran-Morales E, Estrada-Martinez S, Liessenfeld O, Marquez AJ. Seroepidemiology of infection with Toxoplasma gondii in health blood donors of Durango, Mexico. BMC Infect Dis. 2007; 7:75. doi: 10.1186/1471-2334-7-75.
  19. Góngora-Biachi RA, Gonzalez Martinez P, Castro-Sansores C, Alvarez-Moguel R, Pavía-Ruz N, Lara-Perera D, Alonzo-Salomón G, Palacios-Pérez E. Antibodies against Toxoplasma gondii in patients with HIV in Yucatan. Rev Invest Clin. 1998; 50(5):419–22.
  20. Coelho RA, Kobayashi M, Carvalho J. Prevalence of IgG antibodies specific to Toxoplasma gondii among blood donors in Recife northeast brazil. Rev Inst Med Trop Sao Paulo. 2003;45(4):229–231.
  21. Montoya JG, Remington JS. In: Principles and practice of infectious diseases. 5. Mandell GE, Beneth JE, Dolin R, editor. Odon: Churchill Livingstone; 2000. Toxoplasma gondii; pp. 2858–2888.
  22. Jayawardena S, Singh S, Burzyantseva O, Clarke H. Cerebral toxoplasmosis in adult patients with HIV infection. Clin Med J ResidHosp Physician. 2008; 44(7):17–24.
  23. Fazacli A, Carter PE, Darde ML, Pennington TH. Molecular typing of toxoplasma gondii strains by GRA 6 gene sequence analysis. Int J parasitol. 2000; 30 (5): 637-42.

International Journal of Scientific Research in Science and Technology

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Published

2021-10-30

Issue

Volume 8, Issue 5, September-October-2021

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Research Articles

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[1]
Elghazali Mohammed, Mustafa Yassin, Khalid Anan, Awadalkareem Omer, Mutaz A. Elsir, Abdelrahim M. ElHussein, Alamin Abdalkareem, Osman Mansour "Sero-Molecular Detection of Toxoplasma Gondii Infection among HIV-Positive Patients in Khartoum : A Cross Sectional Study" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 8, Issue 5, pp.125-131, September-October-2021. Available at doi : https://doi.org/10.32628/IJSRST218524