Treatment of Textile Dye Wastewater using Cynobacteria Spirulina Sp
DOI:
https://doi.org/10.32628/IJSRST218529Keywords:
Bioremediation, Real textile dye wastewater, Azo dyes, Cyanobacteria, PhytotoxicityAbstract
Along with toxic dyes textile dye wastewater contains various xenobiotic compounds therefore their treatment is necessary before discharging into the environment. Bioremediation was considered as one of the eco-friendly and self-sustainable treatment systems and amongst them cyanobacteria was considered as one of the best resources for the bioremediation of organic pollutants. Thus, in this present study, Spirulina sp. was evaluated for the treatment of real textile dye wastewater (RTDW) along with the removal of metal ions and other organic pollutants. The metabolic activities to Spirulina sp. leads to complete more than 98% decolorization of Reactive Brown GR11, Reactive Magenta HBB 26, Reactive Red Bs11 and Reactive Yellow 160 dye. However, to enhance the treatment efficiency of Spirulina sp. carbon and nitrogen sources were optimized. In the presence of 1% glucose and yeast extract Spirulina sp. showed 86% American Dye Manufacturers’ Institute (ADMI) removal and 83 % chemical oxygen demand (COD) reduction from the undiluted non-sterilize RTDW. The maximum treatment efficiency of Spirulina sp. was observed at pH-7 and 30 °C under optimized co-substrates. Degradation of RTDW and representative four azo dyes were further confirmed by using ultraviolet–visible spectrophotometry (UV–vis), High Performance Liquid Chromatography (HPLC), and Fourier- transform infrared spectroscopy (FTIR) analysis. Additionally, toxicity analysis was considered as the best method to evaluate the efficiency of treatment process. Phytotoxicity and cytotoxicity assay clearly show that Spirulina sp. treatment significantly reduces the toxic characteristics of RTDW.
References
- APHA, 2005. Standard Methods for the Examination of Water and Wastewater, twentyfirst ed. American Public Health Association, Washington DC.
- Abd Zaid, A. M., Hashim, A. J., & Alkhozai, Z. M. (2015). Dyes decolorization by spore-bound laccase from local isolate of Bacillus subtilis. International Journal, 3(10), 1417-1424.
- Afreen, S., Shamsi, T. N., Baig, M. A., Ahmad, N., Fatima, S., Qureshi, M. I., ... & Fatma, T. (2017). A novel multicopper oxidase (laccase) from cyanobacteria: purification, characterization with potential in the decolorization of anthraquinonic dye. PloS one, 12(4), e0175144.
- Blumel S., Knackmuss HJ. And Solz A. (2002). Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F. Applied and Environmental Microbiology 68: (8): 3948-3955.
- Chen, F., Zhang, Y., & Guo, S. (1996). Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture. Biotechnology letters, 18(5), 603-608.
- Cetin, D., & Dönmez, G. (2006). Decolorization of reactive dyes by mixed cultures isolated from textile effluent under anaerobic conditions. Enzyme and Microbial Technology, 38(7), 926-930.
- Govindwar, S.P., Kurade, M.B., Tamboli, D.P., Kabra, A.N., Joo, P., Waghmode, T.R.,2014. Chemosphere Decolorization and degradation of xenobiotic azo dye ReactiveYellow-84A and textile effluent by Galactomyces geotrichum. Chemosphere 109, 234–238.
- Kabra, A. N., Khandare, R. V., & Govindwar, S. P. (2013). Development of a bioreactor for remediation of textile effluent and dye mixture: a plant– bacterial synergistic strategy. Water research, 47(3), 1035-1048.
- Kalyani, D,C., Telke, A,A., Dhanve, R,S., & Jadhav, J,P. (2009). Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. Journal of Hazardous Materials 163: 735–742.
- Lin, S. H., & Peng, C. F. (1994). Treatment of textile wastewater by electrochemical method. Water research, 28(2), 277-282.
- Moutaouakkil A., Zeroual Y., Dzayri F.Z., Talbi M., Lee K. and Blaghen M. (2003). Purification and partial characterization of azoreductase from Enterobacter agglomerans. Archieves of Biochemistry and Biophysics.413: 139-146.
- N. Azbar, Comparison of various advanced oxidation processes and chemical treatment methods for COD and color removal from a polyester and acetate fiber dyeing effluent, 55 (2004) 35–43. doi:10.1016/j.chemosphere.2003.10.046.
- N. Han, H. Hao, T. Urase, K.Y. Gin, Bioresource Technology A critical review on characterization strategies of organic matter for wastewater and water treatment processes, Bioresour. Technol. 193 (2015) 523–533. doi: 10.1016/j.biortech.2015.06.091.
- Solís, M., Solís, A., Pérez, HI., Manjarrez, N., & Floresa, M. (2012). Microbial decolouration of azo dyes: A review. Process Biochemistry 47 (2012) 1723–1748.
- Sheth, N., & Dave, S. (2010). Enhanced biodegradation of Reactive Violet 5R manufacturing wastewater using down flow fixed film bioreactor. Bioresource technology, 101(22), 8627-8631.
- Saratale, R.G., Saratale, G.D., Chang, J.S., Govindwar, S.P., 2010. Decolorization andbiodegradation of reactive dyes and dye wastewater by a developed bacterial consortium. Biodegradation 21 (6), 999–1015. https://doi.org/10.1007/s10532-010-9360-1.
- Teli, M. D. (2008). Textile coloration industry in India. Coloration Technology, 124(1), 1-13.
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