Insilico Search for Potential DNA Binding Domain of Human Zinc Finger Protein Sp1

Authors

  • Sirisha Kaniganti  International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, India and Department of Biotechnology, Osmania university, Hyderabad, India

DOI:

https://doi.org/10.32628/IJSRST218615

Keywords:

GC-box, Zinc finger motifs, SP1, Transcriptional regulation.

Abstract

Specificity protein 1 (Sp1) belongs to a family of ubiquitously expressed, C2H2-type zinc finger-containing DNA binding proteins that activate or repress transcription of many genes in response to physiological and pathological stimuli. Specificity protein 1 is considered to be a constitutively expressed transcription factor and has been implicated in the regulation of a wide variety of housekeeping genes, tissue-specific genes, and genes involved in the regulation of growth. In order to determine the binding affinity of Sp1 zinc finger domains, the total energy for each and every possible combination of GC box and Zn finger motifs using Hex server, Model IT software’s is calculated. According to the findings of this study, the design of multi-zinc finger proteins with a variety of sequence specificities will be easier to accomplish. Among the three motifs present in Specificity protein 1, motifs 1 and 2 have higher binding affinity than motif 3.

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International Journal of Scientific Research in Science and Technology

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Published

2021-12-30

Issue

Volume 8, Issue 6, November-December-2021

Section

Research Articles

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How to Cite

[1]
Sirisha Kaniganti "Insilico Search for Potential DNA Binding Domain of Human Zinc Finger Protein Sp1" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 8, Issue 6, pp.217-225, November-December-2021. Available at doi : https://doi.org/10.32628/IJSRST218615