Effect of Salinity on Catalase and Peroxidase Activity of Chlorella vulgaris Beijerink

Authors

  • Shaila Hiremath  Department of Botany, Sharnbasveshwar College of Science, Kalaburagi, Karnataka, India
  • Pratima Mathad  Department of P.G. Studies and Research in Botany, Gulbarga University, Kalaburagi, Karnataka, India

DOI:

https://doi.org//10.32628/IJSRST229613

Keywords:

Chlorella, Catalase, Peroxidase, NaCl

Abstract

In the present work, the effect of NaCl on catalase and peroxidase activity of Chlorella vulgaris Beijerink was investigated The C. vulgaris was treated with different concentrations of NaCl viz., 0.1, 0.2, 0.3 and 0.4M besides control over 10, 20 and 30 days. The results exhibited increase in the catalase and peroxidase activity up to 0.3 M, whereas it was decreased at 0.4M for all the cultures over all the durations. The study revealed that, increased activity of catalase and peroxidase was an adaptive mechanism to reduce the H2O2 and offer protection against oxidative damage and tolerance against salt.

References

  1. Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Murata, N., 2000. Inactivation of photosystems I and II in response to osmotic stress in Synechococcus: contribution of water channels. Plant Physiol., 122:1201–1208
  2. Ashraf, M., 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol. Adv., 27: 84–93.
  3. Bohnert, H.J. and Jensen, R.G. 1996. Metabolic engineering for increased salt tolerance the next step. Aust. J. Plant Physiol. 23: 661-66.
  4. Bohnert, H.J. and Sheveleva, E. 1998. Plant stress adaptations-making metabolism move. Curr. Opin. Plant. Biol. 1: 267-274.
  5. Fogg, G. E., 2001. Algal adaptation to stress - some general remarks, in Rai, L. C., Gaur, J. P. (Eds.), Algal adaptation to environmental stresses, Springer, Berlin, 1 – 19.
  6. Sairam, R.K., & Tyagi.A., Physiological and molecular biology of salinity stress tolerance in plants, Curr., Sci.,86(2004) 407-420.
  7. Cavalcanti, F. R., Lima, J., Ferreira-Silva, S. L., Viégas, R. A. and Silveira, J. (2007) Roots and leaves display contrasting oxidative response during salt stress and recovery in cow pea. . Journal of Plant Physiology, 164, 591-600.
  8. Hasegawa, P.M., Bressan, R.A., Zhu, J.K., and Bohnert, H.J. (2000) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology., 51, 463-499.
  9. Hoque, M.A., Okuma, E., Banu, M.N.A., Nakamura, Y., Shimoishi, Y., and Murata, Y. (2007) Exogenous proline m es the detrimental effects of salt stress more than the betaine by increasing antioxidant enzyme activities. J. Plant Physiol., 164, 553- 561.
  10. Agarwal, S. and Pandey, V., 2004. Antioxidant enzyme response to NaCl stress in Cassia angustifolia. Biol. Plantarum., 48: 555- 560.
  11. Chakraborthy S, Santra S C, Bhattacharya T,(2010): Seasonal variation of enzyme activity and stress metabolites in eight benthic macro algae with fluctuations in salinity of sunderban estuary, India. Indian Journal of Marne Sciences, Vol. 39(3), September 2010, pp 429-433.
  12. Teranishi, Y., Tanaka, A., Osumi, M., Fukui, S., 1974. Catalase activity of hydrocarbon utilizing Candida yeast. Agric. Biol. Chem., 38: 1213-1216.
  13. Castillo, F. I., Penel, I. and Greppin, H., 1984. Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol., 74:846–851.
  14. Jaleel, C. A., Sankar, B., Sriaharan, R. and Panneerselvam, R., 2008. Soil salinity alter growth, chlorophyll content and secondary metabolite accumulation in Catharanthu roseus, Turk. J. Biol., 32: 79-83.
  15. Fridivich, I., 1989. Superoxide dismutases: An adaptation to a paramagnetic gas, The Journal of Biological Chemistry, 264:7761-7764.
  16. McCord, J. M., Fridovich, I., 1969. Superoxide dismutase: An enzymic function for reythrocuprein (Hemocuprein), The Journal of Biological Chemistry,244: 6049- 6055.
  17. Yamazaki, J., Ohashi, A., Hashimoto, Y., Negishi, E., Kumagai, S., Kubo, T., Oikawa, T., Maruta, E., Kamimura, Y., 2003. Effects of high light and low temperature during harsh winter on needle photodamage of Abiesmariesii growing at the forest limit on Mt. Norikura in Central Japan. Plant Science, 165: 257-264.
  18. Jahnke, L. S. and White, A. L., 2003. Long term hyposaline and hypersaline stresses produce distinct antioxidant response in the Dunaliella tertiolecta, Pl. Physiol., 160:1193- 1202.
  19. Chakraborthy S, Santra S C, Bhattacharya T,(2010): Seasonal variation of enzyme activity and stress metabolites in eight benthic macro algae with fluctuations in salinity of sunderban estuary, India. Indian Journal of Marne Sciences, Vol. 39(3), September 2010, pp 429-433.
  20. Wai, S. N., Mizunoe, Y., Takade, A. and Yoshida, S., 2000. A comparison of solid and liquid media for resuscitation of starvation- and low temperature-induced non culturable cells of Aeromonas hydrophila. Arch Microbiol., 173: 307–310.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2022-12-30

Issue

Volume 9, Issue 6, November-December-2022

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Shaila Hiremath, Pratima Mathad, " Effect of Salinity on Catalase and Peroxidase Activity of Chlorella vulgaris Beijerink, International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 9, Issue 6, pp.189-193, November-December-2022. Available at doi : https://doi.org/10.32628/IJSRST229613