Isolation and Comparative Kinetics Study of Bromelain from Different Parts of Pineapple

Authors

  • Ishita Bhattacharya   Department of Life Science, Binod Bihari Mahto Koyalanchal University, Dhanbad, India

Keywords:

Bromelain, GDU, Gelatin, Kinetics

Abstract

Bromelain is a general name for a family of sulfhydryl l containing Proteolytic enzymes obtained from Ananas comosus, the pineapple plant. It is present in large quantities in fruit, leaves and stems of several varieties of pineapple. Pineapple is a good source for manganese and contains significant amount of vitamins c and B1.it contains the proteolytic enzymes Bromelain which is used as a meat tenderizing agent. Bromelain obtained from the stems of the pineapple plant contains all the soluble components of the pineapple stem in their original properties, which may involve malignant cell growth, thrombus formation, inflammation, control of diarrhea, dermatological and skin debridement. The present research investigation is focused towards the comparative study of Bromelain and its enzymatic activity which are present in fruit, peel and leaf part of pineapple. Bromelain extract was used to determine the rate at which gelatin was degraded. Activity of one-gram Bromelain is equivalent to 1,200 GDU. Gelatin was chosen as the substrate for the analysis of activity of Bromelain. In case of enzymes isolated from fruit of pineapple elute 1 had the highest activity with 560 units/mL (Table 5). Like wise in case of peel and leaf extracts of Bromelain were highest with 560 units/mL and 583 units/mL respectively.

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International Journal of Scientific Research in Science and Technology

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Published

2023-11-24

Issue

Volume 10, Issue 6, November-December-2023

Section

Research Articles

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How to Cite

[1]
Ishita Bhattacharya , " Isolation and Comparative Kinetics Study of Bromelain from Different Parts of Pineapple , International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011, Volume 10, Issue 6, pp.556-567, November-December-2023.