Development and Validation of Single Laboratory Ultra Fast Liquid Chromatography Method for Quantification of       Buprofezin in Suspension Concentrate (SC) Formulation

Shubham Yadav; Samsul Alam; Ajin S. Anil; Lalitesh K. Thakur

doi:10.32628/IJSRST52310377

Authors

Shubham Yadav Analytical Science Division, Institute of Pesticide Formulation Technology, Gurugram, Haryana, India
Samsul Alam Analytical Science Division, Institute of Pesticide Formulation Technology, Gurugram, Haryana, India
Ajin S. Anil Analytical Science Division, Institute of Pesticide Formulation Technology, Gurugram, Haryana, India
Lalitesh K. Thakur Analytical Science Division, Institute of Pesticide Formulation Technology, Gurugram, Haryana, India

DOI:

https://doi.org//10.32628/IJSRST52310377

Keywords:

Buprofezin res; Horwitz equation; Method validation, CIPAC - Collaborative International Pesticides Analytical council, Uncertainty in measurements.

Abstract

A novel ultra fast liquid chromatography (UFLC) method has been developed and validated for quantification of Buprofezin in Suspension Concentrate (SC) formulation, using Shimadzu, packed with C18 (250 mm × 4.6 mm, 5 μm) column. Mixture of acetonitrile:water (80:20 v/v) was used as mobile phase. The flow rate was kept 1.5 ml/min and detection was carried out at 250 nm. The linearity of proposed method was investigated in the range of 200-1002 PPM (r2=0.999). The percentage recovery found to be 99.87%. The % RSD value for precision study was <0.68 as per modified Horwitz equation as requirements by CIPAC. The developed method was found to be specific, linear, precise and accurate. This method is also useful for quantification of Buprofezin in their single or combination formulated products, environmental samples (soil, water), and agricultural products for pesticide residue analysis.

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