Dissociation Constant of Proline in 10, 20 and 30% Dioxane in Dioxane-Water at 288.15K and Related Thermodynamic Quantities by e.m.f. Measurement
DOI:
https://doi.org/10.32628/IJSRST52310478Keywords:
Proline, Thermodynamic Quantities, Biomolecule, Barometric Pressure, Vapour Pressure, Bubbler DepthAbstract
Dissociation constant of Proline was determined by potentiometric method using following type of cell:
H2(Pt) | Proline, HCl, X% Dioxane, Hg2Cl2 | Hg ...(C-1)
m1 m2(100-x)%water
and emf of the cell is given by:
E = E0 − (2.303RT/F) (log mH+ mCl_ + log ƔH+ ƔCl−) ...(1)
where
log ƔH+ ƔCl_ = -(2A`√μ/(1+√μ) )+ β1μ (Modified Davies Equation) ...(2)
The equation (2) is Modified Davies Equation which is used for calculating activity coefficient in a system and which is measure of deviation from ideality of solution. Hence, taking into account activity coefficient, acidic dissociation constant of Proline can be calculated by calculating mH+ given by the formula:
log mH+ = (E0 − E)/K − log mCl_ + (2A’ √μ/(1+ √μ)) − β1μ ...(3)
and hence acidic dissociation constant of Proline is given by:
logK1 − β1μ = logK1(A) − (2A`√μ) / (1 + √μ) and hence by calculating acidic dissociation constant of Proline, thermodynamic quantities ΔG0 , ΔH0 and ΔS0 can be calculated.
References
- H. S. Harned and B. B. Owen, J. Am. Chem. Soc., 1930, 52(12), 5091-5102.
- H. S. Harned and B. B. Owen, Chem. Rev., 1939, 25(1), 31-65.
- B. B. Owen, J. Am. Chem. Soc., 1934, 56(8), 1695-1697.
- L. F. Nims and P. K. Smith, J. Bio. Chem., 1933, 101(2), 401-412.
- P. K. Smith and A. C. Taylor and E. R. B. Smith, J. Bio. Chem., 1937, 122(1), 109-123.
- J. T. Edsall and R. D. Blanchard, J. Am. Chem. Soc., 1933, 55(6), 2337-2353.
- A. K. Chttopadhyay, S. C. Lahri, Indian. J. Chem., 1977, 15A, 930.
- H. S. Harned and C. M., Birdsall, J. Am. Chem. Soc., 1943, 65(1), 54-57.
- A.K. Sinha, J.C. Ghosh and B. Prasad, Indian J. Chem. A, 1981, 20(01), 33-35.
- G. Sahu and Prasad B, Indian J. Chem. Soc, 1969, 46, 233.
- B. Prasad, J. Indian. Chem. Soc., 1977, 54, 588.
- A. Gupta, Ph.D Thesis, Patna University, Patna, 1986.
- S. Ghosh, Ph.D Thesis, Patna University, 1992.
- H. S. Harned and R. A. Robinson, Trans. Fared. Soc., 1940, 36, 973-978.
- B. M. Prasad and J. C. Gosh, Proc. Nat. Acad. Sc. India, 1990, 60A, III, 255.
- C. F. Well, J. Chem. Soc. Faraday Trans.- I, 1976, 72, 601-609.
- C. F. Well, J. Chem. Soc. Faraday Trans.-I, 1973, 69, 984-992.
- D. Feakins, Physico-chemical process and mixed aqueous solvents, Edited by F. Franks (Hienemann Educational Books Ltd., London), Jan. 1967, 7190.
- Born.M. ; Z Physik, 1920, 1, 45
- Sager, E.E.Robinson, R.A. and Bates, R.G. ; J. Res.Nat.Bur.Stand, 1965, A69, 263
- Parson,G.H. and Rochester;C.H. ; J.Chem.Soc.; Farad I; 1975, 71, 1068
- Chattopadhyay, A.K, and Lahiri, S.C.; Electrothermica Acta, 1982, 27, 269
- H. S. Frank and M. W. Evans, J. Chem. Phys., 1945, 13(11), 507-532.
- D. Feakins and P. Watson., J. Chem. Soc. (Resumed), 1963, 4734-4741.
- F. Frank, “Water, A Comprehensive Treatise” Vol. II, Edited by F. Frank, Plenum Press, New York, London, 1973, 1430.
- H. S. Frank and W. Y. Wen, Disc. Farad. Soc., 1967, 24, 133-1
Downloads
Published
Issue
Section
License
Copyright (c) IJSRST
This work is licensed under a Creative Commons Attribution 4.0 International License.