In Silico Analysis and Molecular Modelling of Neutrophil cytosol factor 4/p40phox involved in Chronic Granulomatous Disease

Abstract

This disease first described in the year 1950s.Immune deficiencies are the condition which the body’s immune cells are unable to kill any foreign body. In this disease the collection of granulomas/small nodules occurs granulomas (collection of immune cells) i.e. phagocytes when they cluster these phagocytes are unable to kill any pathogen.CGD patients, with a phagocyte NADPH oxidase that is not properly functioning, suffer from recurrent, life-threatening infections with certain bacteria, fungi, and yeasts. Defects occurs in one of the five subunits of NADPH Oxidase. It is caused by mutations in genes encoding protein subunits of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. CGD is characterized by a defective intracellular killing of phagocytosed organisms due to a defective oxidative burst in the neutrophils and macrophages. Includes following proteins gp91phox(CYBB gene),p22phox (CYBA gene), p47phox (NCF1), p67phox (NCF2), p40phox(NCF4). So now in present studies we have used different Insilicotools and techniques which includes retrieval of Neutrophil cytosol factor 4 sequence from UniProt database and physiochemical parameters analysed by using Protparam tool. In that leucine had maximum amino acid composition. The structure of protein has very important role in its function.The secondary structure was predicted by using SOPMA tool which indicated that the percentage of Random coils was higher than the percentage of alpha helix and extended strand. Then the 3D structure of Neutrophil cytosol factor 4 was predicted by using SWISS MODEL server and the model was validated by using PROCHECK analysis after validation of the model, the validation score was 93.6%.