A study on the anti-oxidant and anti-cancer activity of Pleiogynium solandri (Benth.) Engl. against ovarian cancer cells (SK-OV-3)

Authors

  • Shirisha. A  Department of Post-graduate Studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • Mohan Kumar S. B  Department of Post-graduate Studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • Getteboina Prasad  Department of Post-graduate Studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • Kiran Kumar D. J  Associate Professor, Department of post-graduate Studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India

Keywords:

Pleiogynium solandri (Benth.) Engl, GC-MS Analysis, SKOV3, Flow cytometry, cytotoxic assay

Abstract

The Present study was focused on evaluating the Antioxidant and cytotoxic potentials of methanolic extract of different parts of Pleiogynium solandri (Benth.) Engl. The GC/MS analysis of the Pleiogynium solandri leaves revealed the presence of seven Bioactive compounds proven to have significant therapeutic effects including Antioxidant, Antimicrobial, Anti-inflammatory and anti-tumorigenic effects inducing apoptosis in ovarian and Breast cancer cells. The Pleiogynium solandri leaf was found to be more potent than bark and fruit regarding its cytotoxic effectiveness against Ovarian cancer cells (SK-OV-3). The Bark extract was found to have a significant antioxidant effect as compared to Leaf and Fruit. The FACS (Fluorescence-activated cell sorting) studies using a Flow cytometer gave cell populations at different stages of the cell cycle affected by test concentrations with propidium iodide as a dye. The FACS analysis showed cell cycle arrest at the 'S' phase of the cell cycle due to the influence of crude drugs.

References

  1. Singh, D., Vignat, J., Lorenzoni, V., Eslahi, M., Ginsburg, O., Lauby-Secretan, B., ... & Vaccarella, S. (2023). Global estimates of incidence and mortality of cervical cancer in 2020: a baseline analysis of the WHO Global Cervical Cancer Elimination Initiative. The Lancet Global Health, 11(2), e197-e206.
  2. Jaryum, K. H., Aliyu, R., Deme, A. S., Daniang, I. E., & Binjin, D. N. (2008). Cancer genetics–A review. International Journal of Biological and Chemical Sciences, 2(4), 587-591.
  3. Oikonomou, E., Anastasiou, Μ., Siasos, G., Androulakis, E., Psyrri, A., Toutouzas, K., & Tousoulis, D. (2018). Cancer Therapeutics-Related Cardiovascular Complications. Mechanisms, Diagnosis and Treatment. Current pharmaceutical design, 24(37), 4424–4435. https://doi.org/10.2174/1381612825666190111101459
  4. Luu, A. Z., Chowdhury, B., Al-Omran, M., Teoh, H., Hess, D. A., & Verma, S. (2018). Role of Endothelium in Doxorubicin-Induced Cardiomyopathy. JACC. Basic to translational science, 3(6), 861–870. https://doi.org/10.1016/j.jacbts.2018.06.005.
  5. Surendiran, A., Balamurugan, N., Gunaseelan, K., Akhtar, S., Reddy, K. S., & Adithan, C. (2010). Adverse drug reaction profile of cisplatin-based chemotherapy regimen in a tertiary care hospital in India: An evaluative study. Indian journal of pharmacology, 42(1), 40–43. https://doi.org/10.4103/0253-7613.62412
  6. Pleiogynium solandri (Benth.) Engl. (n.d.). Www.gbif.org. Retrieved July 18, 2023, from https://www.gbif.org/species/3661607.
  7. Said, A., Abuotabl, E. A., Raoof, G. F. A., Huefner, A., & Nada, S. A. (2015). Phenolic contents and bioactivities of pericarp and seeds of Pleiogynium solandri (Benth.) Engl. (Anacardiaceae). Iranian journal of basic medical sciences, 18(2), 164.
  8. Abdel Raoof, G. F., Said, A., Ismail, S., & El-anssary, A. A. (2021). A New Insight into Pleiogynium timorense leaves: A focus on the anticancer and antimicrobial potentials. Egyptian Journal of Chemistry, 64(3), 1541-1551.
  9. Raoof, G. F. A., Said, A. A., Mohamed, K. Y., & Gomaa, H. A. (2019). Phytoconstituents and bioactivities of the bark of Pleiogynium timorense (DC.) Leenh (Anacardiaceae). Journal of Herbmed Pharmacology, 9(1), 20-27.
  10. Said, A., Omer, E., El Gendy, M., Abdel Raoof, G., Abd EL-Kader, A., & Fouad, R. (2018). Volatile constituents and cytotoxic activity of the fruits of Pleiogynium timorense (Dc.) Leenh. J Mater Environ Sci, 9(8), 2274-9.
  11. Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603-608.
  12. Rajakumar, D. V., & Rao, M. N. A. (1994). Antioxidant properties of dehydrozingerone and curcumin in rat brain homogenates. Molecular and cellular biochemistry, 140, 73-79.
  13. Denizot, F., & Lang, R. (1986). Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of immunological methods, 89(2), 271-277.
  14. Jackman, J., & O'Connor, P. M. (1998). Methods for synchronizing cells at specific stages of the cell cycle. Current protocols in cell biology, (1), 8-3.
  15. Toor, R. H., Khan, Z. N., Tariq, M., Tassaduq, R., Gardner, Q. A., Waheed-Uz-Zaman, W., Lian, J. B., Stein, J. L., Stein, G. S., & Shakoori, A. R. (2020). Bioactivity-Guided Isolation and Identification of Anti-adipogenic Constituents from the n-Butanol Fraction of Cissus quadrangularis. Critical reviews in eukaryotic gene expression, 30(6), 519–541. https://doi.org/10.1615/CritRevEukaryotGeneExpr.2020036843.
  16. Owczarczyk-Saczonek, A., Lahuta, L. B., Ligor, M., Placek, W., Górecki, R. J., & Buszewski, B. (2018). The Healing-Promoting Properties of Selected Cyclitols-A Review. Nutrients, 10(12), 1891. https://doi.org/10.3390/nu10121891
  17. Siracusa, L., Napoli, E., & Ruberto, G. (2022). Novel Chemical and Biological Insights of Inositol Derivatives in Mediterranean Plants. Molecules (Basel, Switzerland), 27(5), 1525. https://doi.org/10.3390/molecules27051525
  18. Wang, X., Zhang, C., & Bao, N. (2023). Molecular mechanism of palmitic acid and its derivatives in tumor progression. Frontiers in Oncology, 13, 1224125.
  19. Carta, G., Murru, E., Banni, S., & Manca, C. (2017). Palmitic acid: physiological role, metabolism and nutritional implications. Frontiers in physiology, 8, 902.
  20. Folquitto, D. G., Swiech, J. N., Pereira, C. B., Bobek, V. B., Possagno, G. C. H., Farago, P. V., ... & Miguel, O. G. (2019). Biological activity, phytochemistry and traditional uses of genus Lobelia (Campanulaceae): A systematic review. Fitoterapia, 134, 23-38.
  21. Marchand D, Grossi V, Hirschler-Rea A, Rontani JF. Regiospecific enzymatic oxygenation of cis-vaccenic acid during aerobic senescence of the halophilic purple sulfur bacterium Thiohalocapsa halophila. Lipids. 2002 Jun;37(6):541-8. doi: 10.1007/s11745-002-0930-2. PMID: 12120951.
  22. Lim, J. N., Oh, J. J., Wang, T., Lee, J. S., Kim, S. H., Kim, Y. J., & Lee, H. G. (2014). trans-11 18:1 vaccenic acid (TVA) has a direct anti-carcinogenic effect on MCF-7 human mammary adenocarcinoma cells. Nutrients, 6(2), 627–636. https://doi.org/10.3390/nu6020627.
  23. Marchand D, Grossi V, Hirschler-Rea A, Rontani JF. Regiospecific enzymatic oxygenation of cis-vaccenic acid during aerobic senescence of the halophilic purple sulfur bacterium Thiohalocapsa halophila. Lipids. 2002 Jun;37(6):541-8. doi: 10.1007/s11745-002-0930-2. PMID: 12120951.
  24. Evans, L. M., Cowey, S. L., Siegal, G. P., & Hardy, R. W. (2009). Stearate preferentially induces apoptosis in human breast cancer cells. Nutrition and cancer, 61(5), 746–753. https://doi.org/10.1080/01635580902825597.
  25. Jayalakshmi, M., Vanitha, V., & Sangeetha, R. (2018). Determination Of Phytocomponents In Ethanol Extract Of Brassica Oleracea—Using Gas Chromatography–Mass Spectroscopy Technique. Asian J. Pharm. Clin. Res, 11(10), 133-136.
  26. Djihane, B., Wafa, N., Elkhamssa, S., Pedro, H. J., Maria, A. E., & Mohamed Mihoub, Z. (2017). Chemical constituents of Helichrysum italicum (Roth) G. Don essential oil and their antimicrobial activity against Gram-positive and Gram-negative bacteria, filamentous fungi and Candida albicans. Saudi Pharmaceutical Journal: SPJ: the official publication of the Saudi Pharmaceutical Society, 25(5), 780–787. https://doi.org/10.1016/j.jsps.2016.11.001.
  27. Boukhatem, M. N., Kameli, A., Ferhat, M. A., Saidi, F., & Mekarnia, M. (2013). Rose geranium essential oil as a source of new and safe anti-inflammatory drugs. Libyan Journal of Medicine, 8(1).
  28. Qi, F., Yan, Q., Zheng, Z., Liu, J., Chen, Y., & Zhang, G. J. J. B. (2018). Geraniol and geranyl acetate induce potent anticancer effects in colon cancer Colo-205 cells by inducing apoptosis, DNA damage and cell cycle arrest. J BUON, 23(2), 346-352.

International Journal of Scientific Research in Science and Technology

Downloads

Published

2023-10-30

Issue

Volume 10, Issue 5, September-October-2023

Section

Research Articles

License

Copyright (c) IJSRST

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
Shirisha. A, Mohan Kumar S. B, Getteboina Prasad, Kiran Kumar D. J "A study on the anti-oxidant and anti-cancer activity of Pleiogynium solandri (Benth.) Engl. against ovarian cancer cells (SK-OV-3)" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 10, Issue 5, pp.189-197, September-October-2023.