Identification and Anticancer Evaluation of Paliasa Leaves (Kleinhovia hospital Linn) Extracts Obtained by Subcritical Extraction

Authors

  • Yetri Elisya  Department of Pharmacy, Poltekkes Kemenkes Jakarta II, Jakarta, Indonesia
  • Junaedi  Department of Pharmacy, Poltekkes Kemenkes Jakarta II, Jakarta, Indonesia
  • Maratu Saleha   Biomedical and Basic Health Technology, R & D Health, Jakarta, Indonesia
  • Ulya Safrina  Department of Pharmacy, Poltekkes Kemenkes Jakarta II, Jakarta, Indonesia

DOI:

https://doi.org/10.32628/IJSRST52411142

Keywords:

Paliasa leaves, identification, anticancer, subcritical extraction, MTT assay

Abstract

Many plants have been used as co-therapy for cancer drugs to minimize the side effects of existing cancer drugs. Cancer drug research is needed to test the potential properties of plants as cancer drugs. Currently, the use of drugs from natural ingredients has begun to develop. However, it is necessary to have a method of extracting raw materials from nature that is practical, cheap, and high-quality. One method that can be used is the extraction of natural materials by subtraction. This research aimed to see how successful this subcritical extraction method is on Paliasa leaves (Kleinhovia hospital Linn). Secondary metabolites extraction, fractionation, and subcritical extraction were then tested on cancer cell lines and determined metabolite profiling using GC/MS and LC/MS/MS. The fractionation process obtained a yield of 33.6%, while the extraction with ethanol yielded 8.84%, and the subcritical extraction yield value was 0.18%. Based on the GC/MS test, the compounds that are predicted to have anti-cancer properties are Isorhamnetin and Carbamic acid derivates group. Meanwhile, from the results of the LSMS-MS test, the 7-Hydroxy-5-methoxycoumarin compound is a coumarin compound derivative and Biatractylolide that has the potential to be an anti-cancer. Subcritical extract of Paliasa leaves has the best IC50 results among the three kinds of extracts tested by MTT assay on MCF-7 cells.

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International Journal of Scientific Research in Science and Technology

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Published

2024-02-29

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Volume 11, Issue 1, January-February-2024

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Research Articles

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[1]
Yetri Elisya, Junaedi, Maratu Saleha, Ulya Safrina "Identification and Anticancer Evaluation of Paliasa Leaves (Kleinhovia hospital Linn) Extracts Obtained by Subcritical Extraction" International Journal of Scientific Research in Science and Technology(IJSRST), Online ISSN : 2395-602X, Print ISSN : 2395-6011,Volume 11, Issue 1, pp.398-409, January-February-2024. Available at doi : https://doi.org/10.32628/IJSRST52411142