Development and Validation of UV-VIS Spectrophotometric Method for Estimation of Methylprednisolone from Dissolution Media

Mhetre M. N.; Kalshetti M. S.

doi:10.32628/IJSRST24113116

Authors

Mhetre M. N. D. S. T. S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India Author
Kalshetti M. S. D. S. T. S. Mandal’s College of Pharmacy, Solapur, Maharashtra, India Author

DOI:

https://doi.org/10.32628/IJSRST24113116

Keywords:

Methylprednisolone, UV-Spectrophotometer, Dissolution, Injectable Suspension

Abstract

A simple, accurate UV-Spectrophotometric method has been developed using a dissolution media, 0.1% Tween 20 as a solvent to determine the methylprednisolone. At a predetermined λmax of 245 nm, method was proved linear in the range of 5-40 μg/ml with good correlation coefficient (R²=0.998) and excellent recovery (between 98 and 101%). This method was successfully applied to the determination of methylprednisolone from dissolution media, 0.1% Tween 20 aqueous solution. This method can be employed for the dissolution study of methylprednisolone in injectable suspension.

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References

Indian Pharmacopeia. Indian Pharmacopeia commission, Ghaziabad. 1996; 1: 482-3.

Laurenc L. Brunton, John S. Lazo, Keith L. Parker; Goodman and Gilman’s the pharmacological basis of Therapeutics; McGraw Hill, Newyork, U.S.A; 2001; 10: 1594, 1603.

Mostafa F. Al-Hakkani, A new validated facile HPLC analysis method to determine Methylprednisolone including its derivatives and practical application. NIH 2023;13:11548. DOI: https://doi.org/10.1038/s41598-023-38539-2

Vijaya Krishna C. Aradhya, Greeshma S. E., A. Satish Kumar Shetty, Anil Kumar. Development and Validation of UV Spectrophotometric Method for the Estimation of Methylprednisolone in Bulk and Pharmaceutical Formulation. WJPPS 2018;7(5):498-503.

ICH-Guidelines Q2 (R1), Validation of Analytical Procedures: Text and Methodology. 2005

Beckett, A. H. Stenlake, J. B., Practical Pharmaceutical Chemistry, 4th edition, CBS Publishers and Distributors, New Delhi, 2002; 2: 275-295.

Connors K. A., A textbook of Pharmaceutical Analysis, 3rd edition, John Wiley and sons, 1982; 221-224.

Kim H, Yoo J Y, Han S B, Lee H J. Determination of ambroxol in human plasma using LC-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 32: 209. DOI: https://doi.org/10.1016/S0731-7085(03)00059-1

ICH, Q2A, Text on Validation of Analytical Procedure: Methodology, International Conference on Harmonization, Geneva, November, 1996; 1-8.

Chan C C, Lee Y C, Lam H, Zhang X M Analytical Method Validation and Instrument Performance Verification, John Wiley and Sons, INC., 2004; 11. DOI: https://doi.org/10.1002/0471463728.ch2