Identification and Antimicrobial Susceptibility Testing of Staphylococcus Aureus from Clinical Samples of Tertiary Care Centre, North India
DOI:
https://doi.org/10.32628/IJSRST24114104Keywords:
Green Synthesis, Schiff base, Thermal Analysis, Antibacterial activityAbstract
Staphylococci are contributors of the Micrococcaceae family. Staphylococcus aureus is the most common type of bacteria which causes infections in the humans. They are classically taken into consideration extracellular, pyogenic pathogens due to their capacity to set off abscess formation. The microorganisms are non-motile and generally tend to develop in clusters. They are extraordinarily hardy and may continue to exist for extended durations of time on environmental surfaces. Staphylococci are identified in medical samples with the aid of using Gram stain and their function morphology – Gram positive cocci in grape-like clusters. They shape spherical frequently beta-hemolytic colonies on agar. It is characterized by the presence of the bacteria in different samples collected from different departments and the use of different antibiotic drugs and other medicines have increased the rate of infections. Some of the other risk factors includes the advanced ages and hospitalization. Staphylococcus aureus can be of different types like MRSA, MSSA, VRSA etc. Staphylococcus can cause infections in different ages it can infect child also or adult also. Different types of samples of Staphylococcus aureus can be identified using MALDI-TOF, MICROSCAN and BD PHOENIX and their interpretation of the antimicrobial susceptibility test can be given. Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of harmful diseases to the clinical manifestations. Infections caused by this bacterium are commonboth in community as well as hospital settings and treatment of the diseases caused by this remains challenging to manage. Because due to the emergence of its multi-drug resistant strainssuch as MRSA. Different drugs were used like Amp/Sulbactam, Ampicilin, Ceftriaxone, Ciprofloxacin, Clindamycin, Daptomycin, Erythomycin, Linezolid, Oxacillin, Rifampin, Synercid, Tetracycline, Teicoplanin, Vancomycin, Penicillin. The antimicrobial susceptibility test was done and their resistance percentage is calculated.
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References
Andrewes, F., & Gordan, M. (1907). report of the biological characters of Staphylococci pathogenic for man. 35th ann. rep. loc. gov. bd. rep. med. off, 548. baron, s. (1996). medical microbiology 4th edition, university of texas. isbn- 10;0-9631172-1-1.
Blair , J. (1958). factors determining the pathogenicity of Staphylococci. annu revmicrobial. DOI: https://doi.org/10.1146/annurev.mi.12.100158.002423
Coates R., Morjan J., & Horsburgh M.J. (2014). Staphylococci colonizer andpathogen of human skin. future microbial. DOI: https://doi.org/10.2217/fmb.13.145
foster, T. (1996). chapter 12 Staphylococcus, medical microbiology, 4th edition.
kloos, W., & schleifer, K. (1986). bergeys manual of systematic bacteriology vol 2 .baltimore.
Laupland K.B., Lytikaninon O., & Sgard M. (1998). the changing epidemology ofStaphylococcus aureus blood stream infection. lowy FD, 520-32.
Lowdy, & FD. (1998 aug 20). Staphylococcus aureus infections. N ENGL J. med, 339. DOI: https://doi.org/10.1056/NEJM199808203390806
Newsom, & Ogstons S.W. (n.d.). coccus J. hosp. infect. 369-372.
rheinbaen, V., & RM, h. (1981). antonie lewenhoek J. microbial. serol. 37,41-51.
Schleifer, K.H., kloos, & W.E. (1975). J. clin. microbial. 337-338. DOI: https://doi.org/10.1128/jcm.1.3.337-338.1975
AO Research Institute, Clavadelerstrasse, CH 7270 Davos, Switzerland, Harris, L., Foster, S., & Richards, R. (2002). An introduction to Staphylococcus aureus, and techniques for identifying and quantifying S. aureus adhesins in relation to adhesion to biomaterials: Review. European Cells and Materials, 4, 39–60. https://doi.org/10.22203/eCM.v004a04 DOI: https://doi.org/10.22203/eCM.v004a04
Cheung, G. Y. C., Bae, J. S., & Otto, M. (2021). Pathogenicity and virulence of Staphylococcus aureus. Virulence, 12(1),547–569. https://doi.org/10.1080/21505594.2021.1878688 DOI: https://doi.org/10.1080/21505594.2021.1878688
Community-Associated Methicillin-Resistant Staphylococcus aureus Infection Among Healthy Newborns—Chicago and Los Angeles County, 2004. (n.d.). Retrieved July 21, 2021, from https://www.cdc.gov/mmwr/preview/mmwrhtml/mm5512a1.htm
Cong, Y., Yang, S., & Rao, X. (2020). Vancomycin resistant Staphylococcus aureus infections: A review of case updating and clinical features. Journal of Advanced Research, 21, 169–176. https://doi.org/10.1016/j.jare.2019.10.005Dalman, M., Bhatta, S., Nagajothi, N., Thapaliya, D., Olson, H., Naimi, H. M., & Smith, DOI: https://doi.org/10.1016/j.jare.2019.10.005
T. C. (2019). Characterizing the molecular epidemiology of Staphylococcus aureus across and within fitness facility types. BMC Infectious Diseases, 19(1), 69. https://doi.org/10.1186/s12879-019-3699-7 DOI: https://doi.org/10.1186/s12879-019-3699-7
Eells, S. J., David, M. Z., Taylor, A., Ortiz, N., Kumar, N., Sieth, J., Boyle-Vavra, S., Daum, R. S., & Miller, L. G. (2014). Persistent environmental contamination with USA300 methicillin-resistant Staphylococcus aureus and other pathogenic strain typesin households with S. aureus skin infections. Infection Control and Hospital Epidemiology, 35(11), 1373–1382. https://doi.org/10.1086/678414 DOI: https://doi.org/10.1086/651678
Experimental treatment approach shows potential against Staphylococcus aureus. (n.d.). ScienceDaily. Retrieved July 22, 2021, from https://www.sciencedaily.com/releases/2019/01/190116140618.htm
Foster, T. J. (1983). Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. Microbiological Reviews, 47(3), 361–409. https://doi.org/10.1128/mr.47.3.361-409.1983 DOI: https://doi.org/10.1128/MMBR.47.3.361-409.1983
Frontiers | Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus | Cellular and Infection Microbiology. (n.d.). Retrieved July 22, 2021, from https://www.frontiersin.org/articles/10.3389/fcimb.2020.00107/full
Identification and treatment of the Staphylococcus aureus reservoir in vivo | Journal ofExperimental Medicine | Rockefeller University Press. (n.d.). Retrieved July 22, 2021, from https://rupress.org/jem/article/213/7/1141/42184/Identification-and- treatment- of-the-Staphylococcus DOI: https://doi.org/10.1084/jem.20160334
Invasive Methicillin-Resistant Staphylococcus aureus Infections in the United States | Infectious Diseases | JAMA | JAMA Network. (n.d.). Retrieved July 22, 2021, from https://jamanetwork.com/journals/jama/fullarticle/209197
Kloos, W. E., & Bannerman, T. L. (1994). Update on clinical significance of coagulase-negative Staphylococci. Clinical Microbiology Reviews, 7(1), 117–140.https://doi.org/10.1128/CMR.7.1.117 DOI: https://doi.org/10.1128/CMR.7.1.117-140.1994
Kot, B., Wierzchowska, K., Piechota, M., & Grużewska, A. (2020). Antimicrobial Resistance Patterns in Methicillin-Resistant Staphylococcus aureus from PatientsHospitalized during 2015–2017 in Hospitals in Poland. Medical Principles and Practice, 29(1), 61–68. https://doi.org/10.1159/000501788 DOI: https://doi.org/10.1159/000501788
Laupland, K. B., Lyytikäinen, O., Sgaard, M., Kennedy, K. J., Knudsen, J. D., Ostergaard, C., Galbraith, J. C., Valiquette, L., Jacobsson, G., Collignon, P., Schnheyder, H. C., & Collaborative, for the I. B. S. (2013). The changing epidemiology of Staphylococcus aureus bloodstream infection: A multinational population-based surveillance study. Clinical Microbiology and Infection, 19(5), 465–471. https://doi.org/10.1111/j.1469-0691.2012.03903.x DOI: https://doi.org/10.1111/j.1469-0691.2012.03903.x
Methicillin resistant Staphylococcus aureus (MRSA) in the intensive care unit | Postgraduate Medical Journal. (n.d.). Retrieved July 22, 2021, from https://pmj.bmj.com/content/78/921/385 DOI: https://doi.org/10.1136/pmj.78.921.385
Methicillin-resistant Staphylococcus aureus. (2021). Wikipedia. https://en.wikipedia.org/w/index.php?title=Methicillin resistant_Staphylococcus_aureus&oldid=1033642099
Methicillin-resistant Staphylococcus aureus: An overview of basic and clinical research | Nature Reviews Microbiology. (n.d.). Retrieved July 21, 2021, from https://www.nature.com/articles/s41579-018-0147-4
Miguel, C. P. V., Mejias, A., Leber, A., & Sanchez, P. J. (2019). A decade of antimicrobial resistance in Staphylococcus aureus: A single center experience. PLOS ONE, 14(2), e0212029. https://doi.org/10.1371/journal.pone.0212029Ondusko, D. S., & Nolt, D. (2018). Staphylococcus aureus. Pediatrics in Review, 39(6),287–298. https://doi.org/10.1542/pir.2017-0224 DOI: https://doi.org/10.1371/journal.pone.0212029
Skin Infections Caused by Staphylococcus aureus | HTML | Acta Dermato- Venereologica. (n.d.)Retrieved July 22, 2021, from https://www.medicaljournals.se/acta/content/html/10.2340/00015555-3466
Skin microbiota: A source of disease or defence? (n.d.). Retrieved July 21, 2021, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746716/
Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management. (n.d.). Retrieved July 22, 2021, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4451395/?report=rea der
Staphylococcus aureus: Overview of Bacteriology, Clinical Diseases, Epidemiology, Antibiotic Resistance and Therapeutic Approach | IntechOpen. (n.d.). Retrieved July 22, 2021, from https://www.intechopen.com/chapters/54154
Stark, L. (n.d.). Staphylococcus aureus—Aspects of pathogenesis and molecular epidemiology. 81.
Taylor, T. A., & Unakal, C. G. (2020). Staphylococcus aureus. In StatPearls [Internet]. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK441868/
Troeman, D. P. R., Van Hout, D., & Kluytmans, J. A. J. W. (2019). Antimicrobial approaches in the prevention of Staphylococcus aureus infections: A review. Journal of Antimicrobial Chemotherapy, 74(2), 281–294. https://doi.org/10.1093/jac/dky421 DOI: https://doi.org/10.1093/jac/dky421
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