Screening of agroresidues for economical and sustainable Phytase Production by Bacteria
DOI:
https://doi.org/10.32628/IJSRST241161100Keywords:
Phytate, Bacterial Phytase, Agroresidues, Cost Effective ProductionAbstract
Phytase, a phytate hydrolysing enzyme has substantial application in food, feed and agriculture sector for improvising the availability of phosphorus and reduces the environmental pollution. The present study emphasizes on cost effective production of bacterial phytase. Total 25 agroresidues were screened for economic production of phytase using bacteria. A total of 30 bacterial isolates were obtained from poultry and goat shed soil and litter sample showing highest phytase activity. The efficient isolates SP-46 and SP-52 were screened for selection of efficient combination bacterial isolate and agroresidues for cost-effective production of phytase. The results showed that utilization of agroresidues not only serve as efficient substrate but also contribute to sustainable agro-waste valorisation and reduces need of expensive synthetic media for phytase production.
Downloads
References
Awad, Ghada E.A., Helal, Mohamed M.I., Danial, Enas N. and Esawy, Mona, A.(2014). Optimization of phytase production by Penicillium purpurogenum GE1 under solid state fermentation by using Box-Behnken design. Saudi Journal of Biological Sci. 21, 81–88., https://doi.org/ 10.1016/j.sjbs.2013.06.004. DOI: https://doi.org/10.1016/j.sjbs.2013.06.004
Bae, H.D., Yanke, L.J., Cheng, K.J. and Selinger, L. B. (1999).A novel staining method for detecting phytase activity. J Microbiol Meth 39, 17-22. DOI: https://doi.org/10.1016/S0167-7012(99)00096-2
Bajaj BK and Wani MA (2011). Enhanced phytase production from Nocardia sp. MB 36 using agro-residues as substrates: Potential application for animal feed production. Eng. Life Sci. 11(6), 620-628 DOI: https://doi.org/10.1002/elsc.201100039
Bakri, Y., Ezzeldeen, H. And Akeed, Y. (2018). Screening of Phytase Producing Fungi Isolated From Syrian Soil. J.Innov. 7(3), 462-474.
Bárbara, E.P. E., Freitas, A. J. De., França, F. S., Salgado, R. L., Guimarães, V. M ., Pereira, F. A. and Eller, M. R. (2019). Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets. Scientific Reports 9, 9256. |https://doi.org/10.1038/s41598-019-45720-z. DOI: https://doi.org/10.1038/s41598-019-45720-z
Bhavsar, K., Shah, P., Soni,S.K. and Khire, J.M. (2008) Influence of pretreatment of agriculture residues on phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions, Afr. J. Biotechnol. 7 8. [100]
Fiske, C.H. And Subbarow, Y. (1925). The colorimetric determination of phosphorus. J Biol. Chem. 66, 375-400. DOI: https://doi.org/10.1016/S0021-9258(18)84756-1
Ho, H.H. Xylanase production by Bacillus subtilis using carbon source of inexpensive agricultural wastes in two different approaches of submerged fermentation (smf) and solid state fermentation. J. Food Process. Technol. 2015, 4, 4–7.
Lee, S.H., Cho, J., Bok, J., Kang, S. Choi, Y. and Lee, P.C.W. (2015) .Characterization, Gene cloning and sequencing of a fungal phytase, PhyA from Penicillium oxalicum PJ3. Prep. Biochem. And Biotech.45, 336-347. DOI: https://doi.org/10.1080/10826068.2014.923446
Mittal, A., Singh, G., Goyal, V., Yadav, A. and Aggarwal, N.(2012). Production of Phytase by Acido-Thermophilic Strain of Klebsiella Sp. DB- 3FJ711774.1 using orange peel flour under submerged fermentation. Innovative Romanian Food Biotechnology 10, 18-27.
Nampoothiri, K. M. Tomes, J., Roopesh, K., Szakacs, G., Nagy, V., Soccol, C.R. and Pandey, A.(2004). Thermostable Phytase Production by Thermoascus aurantiacus in Submerged Fermentation. Applied Biochemistry and Biotechnology 118, 205-214. DOI: https://doi.org/10.1385/ABAB:118:1-3:205
Pandey, A., Soccol, C. R., Nigam, P., & Soccol, V. T. (2000). Biotechnological potential of agro-industrial residues. Bioresource Technology, 74(1), 69–80. DOI: https://doi.org/10.1016/S0960-8524(99)00142-X
Powar, V. K. and Jagannathan, V. (1982). Purification and properties of phytate-specific phosphatase from Bacillus subtilis. J. of Bacteriology 151(3), 1102-1108. DOI: https://doi.org/10.1128/jb.151.3.1102-1108.1982
Selvamohan, T., Ramadas, V. and Rejibeula, M. (2012). Optimization of Phytase Production by Pseudomonas sp. Isolated from Poultry Faces. IJMER. 2(3),1326-1330.
Sheikh, R., kumar, V., Naik, B., Singh, P., Mishra, s., Rustagi, s. and Kumar, V. (2023). Microbial phytase: Their sources, production, and role in the enhancement of nutritional aspects of food and feed additives. Journal of Agriculture and Food Research 12 (2023) 100559. DOI: https://doi.org/10.1016/j.jafr.2023.100559
Shimizu, M. (1992). Purification and Characterization of Phytase from Bacillus subtilis (natto) N–77, Bioscience, Biotechnology, and Biochemistry, 56:8, 1266-1269, DOI: 10.1271/ bbb.56.1266. DOI: https://doi.org/10.1271/bbb.56.1266
Spier, M.R., Greiner,R., Rodriguez-Le´on, J.A., Woiciechowski, A.L., Pandey, A., Soccol, V. T. and Soccol, C.R. (2008). Phytase production using citric pulp and other residues of the agroindustry in SSF by fungal isolates, Food Technol. Biotechnol. 46 : 178–182.
Sreedevi, S. and Reddy, B.N. (2012). Isolation, screening and optimization of phytase production from newly isolated Bacillus sp.C43. IJPBS 2(2), 218-231.
Sumengen, M., Dincer, S. and Kaya, A. (2012). Phytase from Lactobcillus brevis, Turk J Biol 36, 533-541.
Surya, K.K., Vanitha, S., Suresh, S. and Radha, K.V. (2013). Production and optimization of phytase from Rhizopus oligosporus using agro residues by solid state fermentation. Middle-East Journal of Science Research 17(12), 1839-1845.
Tian, M and Yuan, Q. (2016). Optimization of phytase production from potato waste using Aspergillus ficuum, 3 Biotech 6:256. DOI: https://doi.org/10.1007/s13205-016-0573-9
Vohra, A., & Satyanarayana, T. (2003). Phytases: Microbial sources, production, purification, and potential biotechnological applications. Critical Reviews in Biotechnology, 23(1), 29–60. DOI: https://doi.org/10.1080/713609297
Yan, T.Y., Feng R.J., Zhou, D.B., Pan, Y.Y., Chen, Y.F., Wang, F., Yin, L.Y., Zhang, Y.D. and Xie, J.H. (2018). Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere. PLoS ONE 13(11): e0206497. https:// doi.org/10.1371/journal.pone.0206497. DOI: https://doi.org/10.1371/journal.pone.0206497
Downloads
Published
Issue
Section
License
Copyright (c) 2024 International Journal of Scientific Research in Science and Technology
This work is licensed under a Creative Commons Attribution 4.0 International License.