Screening, Identification and Characterization of Protease-Producing Bacteria
DOI:
https://doi.org/10.32628/IJSRST251249Keywords:
Soil sample, Protease- producing bacteria, aloe vera, Skim milk agarAbstract
The ability of bacteria to release enzymes into the environment is widely recognized. Proteases are among the several extracellular enzymes that Bacillus species. which is industrially significant produce. The aim of this work is to identify, characterize and isolate protease producing bacteria from environmental sample. The experiment's required chemicals, reagents, and culture media include nutrients agar, skimmed milk agar, and gram staining (crystal violet, gram iodine, alcohol, safranin) and biochemical reagent. Samples of soil were taken from the rhizosphere of aloe vera. In sterile containers, 10–15 g of soil was gathered at a depth of 5–10 cm, and they were refrigerated before being brought to the lab. Protease-producing bacteria with a variety of proteolytic activity and biochemical characteristics were found to be abundant in aloe vera soil. It may be possible to future optimize protease activity and clarify their structures, which will aid in the discovery of novel proteases.
📊 Article Downloads
References
Chuang, M. H., Chen, J. Y., Wang, H. Y., Jiang, Z. H., & Wu, V. C. (2024). Clinical Outcomes of Tirzepatide or GLP-1 Receptor Agonists in Individuals with Type 2 Diabetes. JAMA network open, 7(8), e2427258. https://doi.org/10.1001/jamanetworkopen.2024.27258
DeVito, N. J., & Drysdale, H. (2022). Issues with reporting and interpretation of Khan et al. 2021. BMC infectious diseases, 22(1), 567. https://doi.org/10.1186/s12879-022-07551-8
Anders, H. J., Saxena, R., Zhao, M. H., Parodis, I., Salmon, J. E., & Mohan, C. (2020). Lupus nephritis. Nature reviews. Disease primers, 6(1), 7. https://doi.org/10.1038/s41572-019-0141-9
Saxena, A., Garan, A. R., Kapur, N. K., O'Neill, W. W., Lindenfeld, J., Pinney, S. P., Uriel, N., Burkhoff, D., & Kern, M. (2020). Value of Hemodynamic Monitoring in Patients with Cardiogenic Shock Undergoing Mechanical Circulatory Support. Circulation, 141(14),1184- 1197. https://doi.org/10.1161/CIRCULATIONAHA.119.043080
Mushtaq, A., Mohd Wani, S., Malik, A. R., Gull, A., Ramniwas, S., Ahmad Nayik, G., Ercisli, S., Alina Marc, R., Ullah, R., & Bari, A. (2023). Recent insights into Nano emulsions: Their preparation, properties and applications. Food chemistry: X, 18, 100684. https://doi.org/10.1016/j.fochx.2023.100684
Boamah, S. A., Spence Laschinger, H. K., Wong, C., & Clarke, S. (2018). Effect of transformational leadership on job satisfaction and patient safety outcomes. Nursing outlook, 66(2), 180–189. https://doi.org/10.1016/j.outlook.2017.10.004
Masi A, Mendoza Diaz A, Tully L, Azim SI, Woolfenden S, Efron D, Eapen V. Impact of the COVID-19 pandemic on the well-being of children with neurodevelopmental disabilities and their parents. J Paediatr Child Health. 2021 May;57(5):631-636. doi: 10.1111/jpc.15285. Epub 2021 Jan 10. PMID: 33426739; PMCID: PMC8014782.
Sneha S, Das MP, Jeyanthi Rebecca L (2014) Isolation and screening of protease producing bacteria from marine waste. J Chem Pharm Res 6:1157–1159.
Masi C, Chandramohan C, Ahmed MF (2017a) Immobilization of the magnetic nanoparticles with alkaline protease enzyme produced by enterococcus hirae and pseudomonas aeruginosa isolated from dairy effluents. Brazilian Arch Biol Technol 60. https://doi.org/10.1590/1678-4324-2017160572
Ahmad, Z. A., Yeap, S. K., Ali, A. M., Ho, W. Y., Alitheen, N. B., & Hamid, M. (2012). scFv antibody: principles and clinical application. Clinical & developmental immunology, 2012, 980250. https://doi.org/10.1155/2012/980250
Harley JP, Prescott LM (2002) Laboratory exercises in microbiology. 5th Ed. The McGraw- Hill companies.
Bergey DH, Holt JG, Krieg NR, Sneath HA (1994) Bergey’s Manual of determinative bacteriology, 9th edn. Williams Wilkins, Baltimore.
Boominadhan U, Rajakumar R, Sivakumaar PKV, Joe MM (2009) Optimization of protease enzyme production using Bacillus Sp. isolated from different wastes. Bot Res Int 2:83–87
Kiran KSP, Satyavani Y, Chandana Lakshmi MVV, Sridevi V (2002) Production of protease enzyme using various sources: review. Res J Biotechnol 7:250–258.
Pandian S, Sundaram J, Panchatcharam P (2012) Isolation, identification and characterization of feather degrading bacteria. Eur J Exp Biol 2:274–282
Mazotto AM, Lage Cedrola SM, Lins U, Rosado AS, Silva KT, Chaves JQ, Rabinovitch L, Zingali RB, Vermelho AB (2010) Keratinolytic activity of Bacillus subtilis AMR using human hair. Lett Appl Microbiol 50(1):89–96. https://doi. org/10.1111/j.1472-765X.2009. 02760.x
MacFaddin, J. F. (2000). Biochemical Tests for Identification of Medical Bacteria (3rd ed.). Lippincott Williams & Wilkins.
Masi, C., Gemechu, G., & Tafesse, M. (2021). Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent. Annals of Microbiology, 71(1). https://doi.org/10.1186/s13213-021-01631-x
Downloads
Published
Issue
Section
License
Copyright (c) 2025 International Journal of Scientific Research in Science and Technology
This work is licensed under a Creative Commons Attribution 4.0 International License.
https://creativecommons.org/licenses/by/4.0
