Screening, Identification and Characterization of Protease-Producing Bacteria

Authors

  • Rohit Kumar Research Intern, Rapture Biotech Pvt. Ltd, Mohali, Punjab, India Author
  • Shivani Research Intern, Rapture Biotech Pvt. Ltd, Mohali, Punjab, India Author
  • Puneet Senior Scientist, Rapture Biotech Pvt. Ltd, Mohali, Punjab, India Author
  • Gurinder Singh Research Associate, Rapture Biotech Pvt. Ltd, Mohali, Punjab, India Author
  • Harpreet Kaur Centre Director, Rapture Biotech Pvt. Ltd, Mohali, Punjab, India Author

DOI:

https://doi.org/10.32628/IJSRST251249

Keywords:

Soil sample, Protease- producing bacteria, aloe vera, Skim milk agar

Abstract

The ability of bacteria to release enzymes into the environment is widely recognized. Proteases are among the several extracellular enzymes that Bacillus species. which is industrially significant produce. The aim of this work is to identify, characterize and isolate protease producing bacteria from environmental sample. The experiment's required chemicals, reagents, and culture media include nutrients agar, skimmed milk agar, and gram staining (crystal violet, gram iodine, alcohol, safranin) and biochemical reagent. Samples of soil were taken from the rhizosphere of aloe vera. In sterile containers, 10–15 g of soil was gathered at a depth of 5–10 cm, and they were refrigerated before being brought to the lab. Protease-producing bacteria with a variety of proteolytic activity and biochemical characteristics were found to be abundant in aloe vera soil. It may be possible to future optimize protease activity and clarify their structures, which will aid in the discovery of novel proteases.

Downloads

Download data is not yet available.

References

Chuang, M. H., Chen, J. Y., Wang, H. Y., Jiang, Z. H., & Wu, V. C. (2024). Clinical Outcomes of Tirzepatide or GLP-1 Receptor Agonists in Individuals with Type 2 Diabetes. JAMA network open, 7(8), e2427258. https://doi.org/10.1001/jamanetworkopen.2024.27258

DeVito, N. J., & Drysdale, H. (2022). Issues with reporting and interpretation of Khan et al. 2021. BMC infectious diseases, 22(1), 567. https://doi.org/10.1186/s12879-022-07551-8

Anders, H. J., Saxena, R., Zhao, M. H., Parodis, I., Salmon, J. E., & Mohan, C. (2020). Lupus nephritis. Nature reviews. Disease primers, 6(1), 7. https://doi.org/10.1038/s41572-019-0141-9

Saxena, A., Garan, A. R., Kapur, N. K., O'Neill, W. W., Lindenfeld, J., Pinney, S. P., Uriel, N., Burkhoff, D., & Kern, M. (2020). Value of Hemodynamic Monitoring in Patients with Cardiogenic Shock Undergoing Mechanical Circulatory Support. Circulation, 141(14),1184- 1197. https://doi.org/10.1161/CIRCULATIONAHA.119.043080

Mushtaq, A., Mohd Wani, S., Malik, A. R., Gull, A., Ramniwas, S., Ahmad Nayik, G., Ercisli, S., Alina Marc, R., Ullah, R., & Bari, A. (2023). Recent insights into Nano emulsions: Their preparation, properties and applications. Food chemistry: X, 18, 100684. https://doi.org/10.1016/j.fochx.2023.100684

Boamah, S. A., Spence Laschinger, H. K., Wong, C., & Clarke, S. (2018). Effect of transformational leadership on job satisfaction and patient safety outcomes. Nursing outlook, 66(2), 180–189. https://doi.org/10.1016/j.outlook.2017.10.004

Masi A, Mendoza Diaz A, Tully L, Azim SI, Woolfenden S, Efron D, Eapen V. Impact of the COVID-19 pandemic on the well-being of children with neurodevelopmental disabilities and their parents. J Paediatr Child Health. 2021 May;57(5):631-636. doi: 10.1111/jpc.15285. Epub 2021 Jan 10. PMID: 33426739; PMCID: PMC8014782.

Sneha S, Das MP, Jeyanthi Rebecca L (2014) Isolation and screening of protease producing bacteria from marine waste. J Chem Pharm Res 6:1157–1159.

Masi C, Chandramohan C, Ahmed MF (2017a) Immobilization of the magnetic nanoparticles with alkaline protease enzyme produced by enterococcus hirae and pseudomonas aeruginosa isolated from dairy effluents. Brazilian Arch Biol Technol 60. https://doi.org/10.1590/1678-4324-2017160572

Ahmad, Z. A., Yeap, S. K., Ali, A. M., Ho, W. Y., Alitheen, N. B., & Hamid, M. (2012). scFv antibody: principles and clinical application. Clinical & developmental immunology, 2012, 980250. https://doi.org/10.1155/2012/980250

Harley JP, Prescott LM (2002) Laboratory exercises in microbiology. 5th Ed. The McGraw- Hill companies.

Bergey DH, Holt JG, Krieg NR, Sneath HA (1994) Bergey’s Manual of determinative bacteriology, 9th edn. Williams Wilkins, Baltimore.

Boominadhan U, Rajakumar R, Sivakumaar PKV, Joe MM (2009) Optimization of protease enzyme production using Bacillus Sp. isolated from different wastes. Bot Res Int 2:83–87

Kiran KSP, Satyavani Y, Chandana Lakshmi MVV, Sridevi V (2002) Production of protease enzyme using various sources: review. Res J Biotechnol 7:250–258.

Pandian S, Sundaram J, Panchatcharam P (2012) Isolation, identification and characterization of feather degrading bacteria. Eur J Exp Biol 2:274–282

Mazotto AM, Lage Cedrola SM, Lins U, Rosado AS, Silva KT, Chaves JQ, Rabinovitch L, Zingali RB, Vermelho AB (2010) Keratinolytic activity of Bacillus subtilis AMR using human hair. Lett Appl Microbiol 50(1):89–96. https://doi. org/10.1111/j.1472-765X.2009. 02760.x

MacFaddin, J. F. (2000). Biochemical Tests for Identification of Medical Bacteria (3rd ed.). Lippincott Williams & Wilkins.

Masi, C., Gemechu, G., & Tafesse, M. (2021). Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent. Annals of Microbiology, 71(1). https://doi.org/10.1186/s13213-021-01631-x

Downloads

Published

03-07-2025

Issue

Section

Research Articles