Effect of Laser and UV Radiations on Germination, Growth and Chlorohyll Content of Brassica juncea

Authors

  • Pranoti R. Khawane Department of Physics, Mahatma Phule Arts and Science College, Patur Dist. Akola, Maharashtra, India Author
  • Dr. Sanjay K. Devade Department of Physics, Shankarlal Khandelwal Arts Commerce and Science College, Akola, Maharashtra, India Author

DOI:

https://doi.org/10.32628/IJSRST2513107

Keywords:

LD 50 value, Glycine max AMS-100-39, Brassica juncea TAM-108, red laser, green laser, UV-A, UV-B, UV-C

Abstract

The present study was conducted to determine the dose of red laser, green laser, UV-A, UV-B and UV-C at which the lethality of the seedling is 50%. For this purpose, the uniform seeds of Brassica juncea (mustard) variety TAM 108 were irradiated with laser and UV radiation at different time intervals. Treated and untreated seeds are kept for germination in two replicates. Accordingly, the LD 50 value of Brassica juncea is determined for the red laser, green laser, UV-A, UV-B and UV-C. The seeds treated with higher LD 50 value were analyzed together with the untreated control seeds for their degree of germination. Afterwads the time for irradiation was chosen and presowing irradiation treatment was given to the seeds and their germination, growth and chlorophyll content was studied for laser and UV radiations. The Red laser shows significant effect on germination growth as well as chlorophyll content follwed by UV A radiations. While, the UV C shows negative effect on growth, germination and chlorophyll content of Brassica juncea.

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References

Harpreet Singh and Amanpreet Singh, “Dynamics of Oilseeds in India: An Overview”, Journal of Pharmacognosy and Phytochemistry,2021

N N Sevostyanova, “Effect of Laser Irradiation on the Process Involved in Growth of Mustard and Radish seeds”, IOP Conf. Series: Earth and Environmental Science, 613, 2020 DOI: https://doi.org/10.1088/1755-1315/613/1/012136

D. Rajarajan “Determination of Lethal Dose and Effect of Gamma Rays on Germination Percentage and Seedling Parameters in ATD (R) 47 Rice”, International Journal of Advanced Biological Research,6(2),328-332, 2016

N.Gupta, S. Sood, Y. Singh and D. Sood , “Determination of lethal dose of gamma rays and ethyl methane sulphonate induced mutagenesis in Cassava”, International Journal of Scientific Research, 2016

N. Khalifa, H. El. Ghandoor, “Investigate the Effect of Nd-Yag Laser Beam on Soybean (Glycin max) Leaves at the Protein Level”, International Journal of Biology,3 (2), 2011. DOI: https://doi.org/10.5539/ijb.v3n2p135

M. Krawiec, A. Dziwulska-Hunek, S. Palonka, M. Kaplan, P. Baryla, “Effect of Laser Irradiation on Seed Germination and Root Yield of Scorzonera”, Acta. Agrophysica, 23(4), 621-631, 201

J. Schmitt and R. Wulff, “Light spectral quality phytochrome and plant competition” Trends in Ecology and Evolution, 8 (2),47-51,1993. DOI: https://doi.org/10.1016/0169-5347(93)90157-K

P. Devlin, Photocontrol of flowering, Blackwell Publication, Oxford UK, 2008

Yunmein Wei et al. “The Role of Light Quality in Regulating Early Seedling Development” Plants 2023,12, 2746 DOI: https://doi.org/10.3390/plants12142746

Jiang Z et al. “Mediated Signaling Controls Seed Dormancy and Germination in Arabidpsis” Nature Communication, 2016,7,12377. DOI: https://doi.org/10.1038/ncomms12377

Park, Y. G., and Jeong, B. R. “Shift in the light quality of night interruption affects flowering and morphogenesis of Petunia hybrida” Plants-Basel 2023, 12, 12. DOI: https://doi.org/10.3390/plants12102049

P. M. C Bueno and W. A. Vendrame, “Wavelength and Light Intensity Affect Macro- and Micronutrient Uptake, Stomata Number, and Plant Morphology of Common Bean (Phaseolus vulgaris L.)” Plants-Basel, 13, 14, 2024. DOI: https://doi.org/10.3390/plants13030441

C. Vatistas, D. Avgoustaki, G. Monedas and T. Bartzanas, “The Effect of Different Light Wavelengths on the Germination of Lettuce, Cabbage, Spinach and Arugula Seeds in a Controlled Environment Chamber” Scientia Horticulturae, 331, 11, 2024. DOI: https://doi.org/10.1016/j.scienta.2024.113118

T. Q. Wang, Q. Y. Sun, Y. J. Zheng, Y. L. Xu, B.B. Liu and Q. M. Li, “Effects of Red and Blue Light on the Growth, Photosynthesis, and Subsequent Growth Under Fluctuating Light of Cucumber Seedlings” Plants-Basel, 13, 19,2024. DOI: https://doi.org/10.3390/plants13121668

Shemah Ali Al—Jehani, Dina Zein El Abdin Abdel Kader and Mohsen Kamal Hassan Ebrahim, “Photobiostimulation of Sesamum Indicumc L. Seeds Using Low Intensity He- Ne Laser Radiation: Seed Germination and Plant Growth” International Research Journal of Natural Sciences 4,35-58, 2016.

Lichtenthaler H. K. and Buschmann, “Extraction of photosynthetic tissues: chlorophylls and carotenoids” Current Protocols in Food Analyical Chemistry,1, 2.1-2.6. DOI: https://doi.org/10.1002/0471142913.faf0402s01

S. Khan and S. Goyal, “Improvement of Mung Bean Varieties Through Induced Mutations” Journal of Plant Science, 3,174-180, 2009.

W. Rybiński, “Influence of laser beams on the variability of traits in spring barley.” International Agrophysics, 14(2), 227-232, 2000.

Yuri Sarreta and Jarbas C de. Castro Neto, “Effects of 660 nm Laser Irradiation of Soybean Seeds On Germination, Emergance and Seedling Growth” Acta. Agrophysica 28, 5-18,2021. DOI: https://doi.org/10.31545/aagr/134841

Bing Liu, Xiao- Bing Liu, Yan Sheng Li and S.J. Herbert, “Effects of enhanced UV-B Radiation on Seeds Growth Characteristics and Yield Components in Soybean”, Field Crop Research 154,158-163, 2013. DOI: https://doi.org/10.1016/j.fcr.2013.08.006

Hussan AraBegum, et al. “Effect of UV Radiation on Germination, Growth, Chlorophyll Content and Fresh and Dry Weights of Brassica rapa. L. and Eureca sativa L.” Sarhad Journal of Agriculture,2021. DOI: https://doi.org/10.17582/journal.sja/2021/37.3.1016.1024

P. Khawane and S. K. Devade, “Determination of LD 50 Value for Red Laser, Green Laser, UV- A, UV-B and UV-C Radiations in Glycine Max, Brassica Nigra and Study of Its Effects on Seed Germination”, IJSRCSEIT,2023.

S. E. Sultan, “Phenotypic plasticity for Plant Development, Function and Life History” Trends in Plant Science, 5: 537–542, 2020. DOI: https://doi.org/10.1016/S1360-1385(00)01797-0

A. Pilura, A. Baliuckiene and V. Baliukas, “Phenogenetic Response of Silver Brich Populations and Half sib families to elevated ozone and ultravoilet-B radiation at juvenile age” Environmental Pollution, 156: 152-161,2008. DOI: https://doi.org/10.1016/j.envpol.2007.12.023

Zongbo Qiu, Mengmeng Yuan, Yanyan He, Yongfang Li and Liang Zhang, “Physiological and Transcriptome analysis of He-Ne Laser Pretreated Wheat Seedlings in Response to Drought Stress” Scientific Reports,7:6108, 2017. DOI: https://doi.org/10.1038/s41598-017-06518-z

R. Neelmegam and T. Sutha, “UV – Cirradiation Effects on Seed Germination, Seedling Growth and Productivity of Groundnut” International Journal of Current Microbilogy and Applied Sciences, 4(8), 430-443, 2015.

K. Ali, N. Khan, I.U. Rahman, W. Khan, M. Ali, N. Uddin and M. Nisar, “The Ethnobotanical Domain of the Swat Valley Pakistan” Journal of Ethnobiology and Ethnomedicine 14(1) 1-15, 2018. DOI: https://doi.org/10.1186/s13002-018-0237-4

F. Kuhulmann and C. Muller, “Development Depended Effects UV Radiation Exposoure on Broccoli Plants and Interaction with Herbivorous Insects” Environmental and Experimetal Botany 66, 61-68, 2009. DOI: https://doi.org/10.1016/j.envexpbot.2009.01.001

Jamal Ahmed Al Zobedy and Jamella Hazza Rasheed, “Enhancement Synthesis of Nuclic Acid Protein and Chlorophyll in Callus of Mustard Brassica” International Journal of Multidisciplinary Research and Growth Evaluation, 2(6),38-43, 2021.

S.S. Shaukat, M. A. Farooq, M.F. Siddiqui and S. A. H. R. Zaidi, “Effect of Enhanced UV B Radiation on Germination, Seedling Growth and Biochemical Process of Vigna Mungo” Pakistan Journal of Botany 45(3), 779-785,2013.

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Published

06-09-2025

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Research Articles

How to Cite

Effect of Laser and UV Radiations on Germination, Growth and Chlorohyll Content of Brassica juncea. (2025). International Journal of Scientific Research in Science and Technology, 12(5), 01-09. https://doi.org/10.32628/IJSRST2513107