Production of Melanin from Bacillus Species Isolated from Rhizosphere Soil
DOI:
https://doi.org/10.32628/IJSRST52411298Keywords:
Melanin, UV, L-tyrosine, L-DOPAAbstract
Melanin is an UV protective pigment which has shown its presence all across the living organisms except for viruses, for various functions such as UV-protection, Anti-microbial activity, free radical scavenging activity etc. The pigment is produced in specialized cells called the Melanocytes by the process of Melanogenesis in humans, while it is produced in cytoplasm in bacteria and other prokaryotes. The main enzyme that is responsible for catalyzing the production of melanin from L-tyrosine is ‘Tyrosinase’. It is this enzyme which enables the bacteria to convert amino acid (L-tyrosine) into melanin. The melanin is a polymer which is made from the L-DOPA, DHI, and DHICA monomers. The current study deals with the production and extraction of melanin from Bacillus species isolated from rhizosphere soil of Neelgiri tree and Sagwan tree. The study further deals with melanin and its characterization using UV- visible spectroscopy, FT-IR spectroscopy and its solubility test in different solvents. Undertaking the industrial applications of bacterial melanin, the current study also deals with the UV-protection activity of the extracted melanin, and the antibacterial activity of the melanin extracted from the bacterial isolated.
Downloads
References
Barate D. L and Sonar N. R (2023). Isolation of tyrosine producing bacteria from rhizosphere soil samples. International Journal for Multidisciplinary Research, 5(3): 1-9 DOI: https://doi.org/10.36948/ijfmr.2023.v05i03.2787
Fava F, Gioia D, Marchetti L (1993). Characterization of pigment produced by Pseudomonas fluorescens during 3-chlorobenzoate co-metabolism, 27(5): 825-835. DOI: https://doi.org/10.1016/0045-6535(93)90014-V
Jigna C, Gordhanbhai PM, Kurian KN (2022). Bacterial melanin with immense cosmetic potential produced by marine bacteria Bacillus pumilius, 1(1): 1-18 DOI: https://doi.org/10.20944/preprints202205.0296.v1
Joshi MH, Patil AA, Adivarekar RV (2021). Characterization of brown pigment isolated from soil bacteria, 1(1): 1-22. DOI: https://doi.org/10.1101/2021.07.21.453201
Korumilli T and Mishra S (2013). Production and characterization of Melanin isolated from Pseudomonas spp, Research Journal of engineering science, 2(5), 40-46.
Pathan AN and Pethe AS (2016). Studies of melanin producing bacteria and extraction of bacterial melanin from sewage samples, International Journal of Applied Research, 2(6): 413-415.
Sajjan SS, Anjaneya O, Kulkarni GB, Nayak AS, Mashetty SB, Karegoudar TB (2013). Properties and functions of melanin pigment extracted from Klebsiella spp, Microbiology and biotechnology letters, 41(1): 60-90. DOI: https://doi.org/10.4014/kjmb.1210.10002
Simon H, Alan C, Nik A, Derek M, Harish B, Smit N (2001). Variations in melanin content and composition in type Ⅴ and type Ⅵ photo exposed and photoprotective human skin: The dominant role of DHI, Pigment cell research, 14(5): 337-347. DOI: https://doi.org/10.1034/j.1600-0749.2001.140505.x
Singh S, Nimse S, Dhimmar A, Sahastrabudhe H, Gajjar A, Kumar P, Shinde P (2021). Microbial melanin and recent advances in biosynthesis, extraction, characterization and application, National library of medicine, Biotechnology and Advances. 1(1): 1-23. DOI: https://doi.org/10.1016/j.biotechadv.2021.107773
Turick CE, Tisa LS, Caccavo Jr (2002) “Production of melanin and uses as soluble electron shutter for Ferrous oxide reduction and as a terminal electron acceptor by Shewanella spp alga, Applied environmental microbiology, 68(5): 2436-2444. DOI: https://doi.org/10.1128/AEM.68.5.2436-2444.2002
Vasanthabhartahi V, Laxminarayan R and Jayalakshmi S (2011). Melanin production from marine Streptomyces, African journal of biotechnology, 10(54): 11220-11234. DOI: https://doi.org/10.5897/AJB11.296
Downloads
Published
Issue
Section
License
Copyright (c) 2024 International Journal of Scientific Research in Science and Technology
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
